Recombinant Anti-uPA Receptor/U-PAR antibody [EPR20191] - BSA and Azide free (ab251557)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20191] to uPA Receptor/U-PAR - BSA and Azide free
- Suitable for: IHC-P, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-uPA Receptor/U-PAR antibody [EPR20191] - BSA and Azide free
See all uPA Receptor/U-PAR primary antibodies -
Description
Rabbit monoclonal [EPR20191] to uPA Receptor/U-PAR - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab251557 is the carrier-free version of ab218106.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20191 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab251557 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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WB |
Use at an assay dependent concentration. Detects a band of approximately 35-65 kDa (predicted molecular weight: 37 kDa).
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Notes |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
Use at an assay dependent concentration. Detects a band of approximately 35-65 kDa (predicted molecular weight: 37 kDa). |
Target
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Function
Acts as a receptor for urokinase plasminogen activator. Plays a role in localizing and promoting plasmin formation. Mediates the proteolysis-independent signal transduction activation effects of U-PA. It is subject to negative-feedback regulation by U-PA which cleaves it into an inactive form. -
Tissue specificity
Expressed in neurons of the rolandic area of the brain (at protein level). Expressed in the brain. -
Sequence similarities
Contains 3 UPAR/Ly6 domains. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 5329 Human
- Omim: 173391 Human
- SwissProt: Q03405 Human
- Unigene: 466871 Human
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Alternative names
- CD 87 antibody
- CD87 antibody
- CD87 antigen antibody
see all
Images
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This data was developed using ab218106, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue labeling uPA Receptor/U-PAR with ab218106 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous staining on dissociative cancer cells in blood vessel of human ovarian cancer is observed [PMID: 25120753]. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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All lanes : Anti-uPA Receptor/U-PAR antibody [EPR20191] (ab218106) at 1/1000 dilution
Lane 1 : Untreated U937 (Human histiocytic lymphoma cell line) whole cell lysate
Lane 2 : U937 whole cell lysate treated with 200nM PMA (Phorbol-12-myristate-13-acetate) for 72 hours
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 37 kDa
Observed band size: 35-65 kDa why is the actual band size different from the predicted?
Exposure time: 15 secondsThis data was developed using ab218106, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
PMA-stimulated U937 cells express uPA Receptor/U-PAR in the range of 35kDa-60kDa. Non-stimulated U937 cells are negative. The deglycosylated form of the protein is at 35 kDa and the highly glycosylated protein is at 60kDa (PMID: 24999729).
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This data was developed using ab218106, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue labeling uPA Receptor/U-PAR with ab218106 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous staining on the macrophages of human ovarian cancer is observed [PMID: 25575713]. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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This data was developed using ab218106, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human lung cancer tissue labeling uPA Receptor/U-PAR with ab218106 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on the stromal cells of human lung cancer is observed [PMID: 25575713]. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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This data was developed using ab218106, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human artery tissue labeling uPA Receptor/U-PAR with ab218106 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on the neutrophils in human artery is observed [PMID: 25575713]. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab251557 has not yet been referenced specifically in any publications.