RecombinantRabMAb

Recombinant Anti-uPA Receptor/U-PAR antibody [EPR20191] - BSA and Azide free (ab251557)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-uPA Receptor/U-PAR antibody [EPR20191] - BSA and Azide free (ab251557)
  • Western blot - Anti-uPA Receptor/U-PAR antibody [EPR20191] - BSA and Azide free (ab251557)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-uPA Receptor/U-PAR antibody [EPR20191] - BSA and Azide free (ab251557)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-uPA Receptor/U-PAR antibody [EPR20191] - BSA and Azide free (ab251557)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-uPA Receptor/U-PAR antibody [EPR20191] - BSA and Azide free (ab251557)
  • Anti-uPA Receptor/U-PAR antibody [EPR20191] - BSA and Azide free (ab251557)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20191] to uPA Receptor/U-PAR - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-uPA Receptor/U-PAR antibody [EPR20191] - BSA and Azide free
    See all uPA Receptor/U-PAR primary antibodies

  • Description

    Rabbit monoclonal [EPR20191] to uPA Receptor/U-PAR - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251557 is the carrier-free version of ab218106. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab251557 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab251557 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
WB
Human
Application Abreviews Notes
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
Use at an assay dependent concentration. Detects a band of approximately 35-65 kDa (predicted molecular weight: 37 kDa).
Notes
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
Use at an assay dependent concentration. Detects a band of approximately 35-65 kDa (predicted molecular weight: 37 kDa).

Target

  • Function

    Acts as a receptor for urokinase plasminogen activator. Plays a role in localizing and promoting plasmin formation. Mediates the proteolysis-independent signal transduction activation effects of U-PA. It is subject to negative-feedback regulation by U-PA which cleaves it into an inactive form.

  • Tissue specificity

    Expressed in neurons of the rolandic area of the brain (at protein level). Expressed in the brain.

  • Sequence similarities

    Contains 3 UPAR/Ly6 domains.

  • Cellular localization

    Secreted and Cell membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • CD 87 antibody
      • CD87 antibody
      • CD87 antigen antibody
      • MO 3 antibody
      • MO3 antibody
      • Monocyte activation antigen Mo3 antibody
      • Plasminogen activator receptor urokinase antibody
      • Plasminogen activator urokinase receptor antibody
      • PLAUR antibody
      • U PAR antibody
      • u plasminogen activator receptor antibody
      • U-PAR antibody
      • u-plasminogen activator receptor form 2 antibody
      • UPA receptor antibody
      • uPAR antibody
      • UPAR_HUMAN antibody
      • Urinary plasminogen activator receptor antibody
      • URKR antibody
      • Urokinase plasminogen activator receptor antibody
      • Urokinase plasminogen activator surface receptor antibody
      • urokinase-type plasminogen activator (uPA) receptor antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-uPA Receptor/U-PAR antibody [EPR20191] - BSA and Azide free (ab251557)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-uPA Receptor/U-PAR antibody [EPR20191] - BSA and Azide free (ab251557)
      This data was developed using ab218106, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue labeling uPA Receptor/U-PAR with ab218106 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous staining on dissociative cancer cells in blood vessel of human ovarian cancer is observed [PMID: 25120753]. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
    • Western blot - Anti-uPA Receptor/U-PAR antibody [EPR20191] - BSA and Azide free (ab251557)
      Western blot - Anti-uPA Receptor/U-PAR antibody [EPR20191] - BSA and Azide free (ab251557)
      All lanes : Anti-uPA Receptor/U-PAR antibody [EPR20191] (ab218106) at 1/1000 dilution

      Lane 1 : Untreated U937 (Human histiocytic lymphoma cell line) whole cell lysate
      Lane 2 : U937 whole cell lysate treated with 200nM PMA (Phorbol-12-myristate-13-acetate) for 72 hours

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 37 kDa
      Observed band size: 35-65 kDa
      why is the actual band size different from the predicted?


      Exposure time: 15 seconds


      This data was developed using ab218106, the same antibody clone in a different buffer formulation.

      Blocking and dilution buffer: 5% NFDM/TBST.

      PMA-stimulated U937 cells express uPA Receptor/U-PAR in the range of 35kDa-60kDa. Non-stimulated U937 cells are negative. The deglycosylated form of the protein is at 35 kDa and the highly glycosylated protein is at 60kDa (PMID: 24999729).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-uPA Receptor/U-PAR antibody [EPR20191] - BSA and Azide free (ab251557)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-uPA Receptor/U-PAR antibody [EPR20191] - BSA and Azide free (ab251557)
      This data was developed using ab218106, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue labeling uPA Receptor/U-PAR with ab218106 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous staining on the macrophages of human ovarian cancer is observed [PMID: 25575713]. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-uPA Receptor/U-PAR antibody [EPR20191] - BSA and Azide free (ab251557)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-uPA Receptor/U-PAR antibody [EPR20191] - BSA and Azide free (ab251557)
      This data was developed using ab218106, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human lung cancer tissue labeling uPA Receptor/U-PAR with ab218106 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on the stromal cells of human lung cancer is observed [PMID: 25575713]. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-uPA Receptor/U-PAR antibody [EPR20191] - BSA and Azide free (ab251557)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-uPA Receptor/U-PAR antibody [EPR20191] - BSA and Azide free (ab251557)
      This data was developed using ab218106, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human artery tissue labeling uPA Receptor/U-PAR with ab218106 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on the neutrophils in human artery is observed [PMID: 25575713]. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
    • Anti-uPA Receptor/U-PAR antibody [EPR20191] - BSA and Azide free (ab251557)
      Anti-uPA Receptor/U-PAR antibody [EPR20191] - BSA and Azide free (ab251557)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab251557? Please let us know so that we can cite the reference in this datasheet.

    ab251557 has not yet been referenced specifically in any publications.

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