Recombinant Anti-uPA Receptor/U-PAR antibody [EPR22173-257] (ab221680)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22173-257] to uPA Receptor/U-PAR
- Suitable for: ICC/IF, Flow Cyt, IP
- Reacts with: Human
Overview
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Product name
Anti-uPA Receptor/U-PAR antibody [EPR22173-257]
See all uPA Receptor/U-PAR primary antibodies -
Description
Rabbit monoclonal [EPR22173-257] to uPA Receptor/U-PAR -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, Flow Cyt, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human uPA Receptor/U-PAR aa 1 to the C-terminus. The exact sequence is proprietary.
Database link: Q03405 -
Positive control
- ICC/IF: U937 cells treated with 200 nM TPA for 72 hours. Flow Cyt: U937 cells treated with 200 nM TPA for 72 hours. IP: U937 treated with 200 nM TPA for 72 hours whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22173-257 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab221680 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/100. | |
| Flow Cyt | 1/500. | |
| IP | 1/30. |
Target
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Function
Acts as a receptor for urokinase plasminogen activator. Plays a role in localizing and promoting plasmin formation. Mediates the proteolysis-independent signal transduction activation effects of U-PA. It is subject to negative-feedback regulation by U-PA which cleaves it into an inactive form. -
Tissue specificity
Expressed in neurons of the rolandic area of the brain (at protein level). Expressed in the brain. -
Sequence similarities
Contains 3 UPAR/Ly6 domains. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 5329 Human
- Omim: 173391 Human
- SwissProt: Q03405 Human
- Unigene: 466871 Human
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Alternative names
- CD 87 antibody
- CD87 antibody
- CD87 antigen antibody
see all
Images
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![Immunocytochemistry/ Immunofluorescence - Anti-uPA Receptor/U-PAR antibody [EPR22173-257] (ab221680)](https://www.abcam.com/ps/products/221/ab221680/Images/ab221680-319490-anti-upa-receptor-u-par-antibody-epr22173-257-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-uPA Receptor/U-PAR antibody [EPR22173-257] (ab221680)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U937 (human histiocytic lymphoma cell line) cells, untreated or treated with TPA (200 nM, 72 hours), labeling uPA Receptor/U-PAR with ab221680 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing membranous staining in U937 cells treated with TPA (200 nM, 72 hours).
The expression of uPA Receptor/U-PAR is induced in PMA-stimulated U937 cells (PMID:24999729).
The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
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![Flow Cytometry - Anti-uPA Receptor/U-PAR antibody [EPR22173-257] (ab221680)](https://www.abcam.com/ps/products/221/ab221680/Images/ab221680-319491-anti-upa-receptor-u-par-antibody-epr22173-257-flow-cytometry.jpg)
Flow Cytometry - Anti-uPA Receptor/U-PAR antibody [EPR22173-257] (ab221680)Flow cytometric analysis of U937 (human histiocytic lymphoma cell line) cell line treated with 200 nM TPA for 72 hours (red) or Untreated control (green) labeling uPA Receptor/U-PAR with ab221680 at 1/500 dilution compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.
The expression of uPA Receptor/U-PAR is induced in PMA-stimulated U937 cells (PMID:24999729)
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![Immunoprecipitation - Anti-uPA Receptor/U-PAR antibody [EPR22173-257] (ab221680)](https://www.abcam.com/ps/products/221/ab221680/Images/ab221680-319492-anti-upa-receptor-u-par-antibody-epr22173-257-immunoprecipitation.jpg)
Immunoprecipitation - Anti-uPA Receptor/U-PAR antibody [EPR22173-257] (ab221680)uPA Receptor/U-PAR was immunoprecipitated from 0.35 mg of U937 (human histiocytic lymphoma cell line) treated with 200 nM TPA for 72 hours whole cell lysate with ab221680 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab221680 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.
Lane 1: U937 treated with 200 nM TPA for 72 hours whole cell lysate 10 µg (Input).
Lane 2: ab221680 IP in U937 treated with 200 nM TPA for 72 hours whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab221680 in U937 treated with 200 nM TPA for 72 hours whole cell lysate.
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 30 seconds.
The expression of uPA Receptor/U-PAR is induced in PMA-stimulated U937 cells and the molecular weight is consistent with what has been described in the literature (PMID: 24999729).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References (0)
ab221680 has not yet been referenced specifically in any publications.
