RecombinantRabMAb

Recombinant Anti-uPA Receptor/U-PAR antibody [EPR22173-257] (ab221680)

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Immunocytochemistry/ Immunofluorescence - Anti-uPA Receptor/U-PAR antibody [EPR22173-257] (ab221680)
  • Flow Cytometry - Anti-uPA Receptor/U-PAR antibody [EPR22173-257] (ab221680)
  • Immunoprecipitation - Anti-uPA Receptor/U-PAR antibody [EPR22173-257] (ab221680)
  • Anti-uPA Receptor/U-PAR antibody [EPR22173-257] (ab221680)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22173-257] to uPA Receptor/U-PAR
  • Suitable for: ICC/IF, Flow Cyt, IP
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-uPA Receptor/U-PAR antibody [EPR22173-257]
    See all uPA Receptor/U-PAR primary antibodies

  • Description

    Rabbit monoclonal [EPR22173-257] to uPA Receptor/U-PAR

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, Flow Cyt, IPmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: U937 cells treated with 200 nM TPA for 72 hours. Flow Cyt: U937 cells treated with 200 nM TPA for 72 hours. IP: U937 treated with 200 nM TPA for 72 hours whole cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab221680 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/100.
Flow Cyt
1/500.
IP
1/30.
Notes
ICC/IF
1/100.
Flow Cyt
1/500.
IP
1/30.

Target

  • Function

    Acts as a receptor for urokinase plasminogen activator. Plays a role in localizing and promoting plasmin formation. Mediates the proteolysis-independent signal transduction activation effects of U-PA. It is subject to negative-feedback regulation by U-PA which cleaves it into an inactive form.

  • Tissue specificity

    Expressed in neurons of the rolandic area of the brain (at protein level). Expressed in the brain.

  • Sequence similarities

    Contains 3 UPAR/Ly6 domains.

  • Cellular localization

    Secreted and Cell membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • CD 87 antibody
      • CD87 antibody
      • CD87 antigen antibody
      • MO 3 antibody
      • MO3 antibody
      • Monocyte activation antigen Mo3 antibody
      • Plasminogen activator receptor urokinase antibody
      • Plasminogen activator urokinase receptor antibody
      • PLAUR antibody
      • U PAR antibody
      • u plasminogen activator receptor antibody
      • U-PAR antibody
      • u-plasminogen activator receptor form 2 antibody
      • UPA receptor antibody
      • uPAR antibody
      • UPAR_HUMAN antibody
      • Urinary plasminogen activator receptor antibody
      • URKR antibody
      • Urokinase plasminogen activator receptor antibody
      • Urokinase plasminogen activator surface receptor antibody
      • urokinase-type plasminogen activator (uPA) receptor antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-uPA Receptor/U-PAR antibody [EPR22173-257] (ab221680)
      Immunocytochemistry/ Immunofluorescence - Anti-uPA Receptor/U-PAR antibody [EPR22173-257] (ab221680)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U937 (human histiocytic lymphoma cell line) cells, untreated or treated with TPA (200 nM, 72 hours), labeling uPA Receptor/U-PAR with ab221680 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

      Confocal image showing membranous staining in U937 cells treated with TPA (200 nM, 72 hours).

      The expression of uPA Receptor/U-PAR is induced in PMA-stimulated U937 cells (PMID:24999729).

      The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    • Flow Cytometry - Anti-uPA Receptor/U-PAR antibody [EPR22173-257] (ab221680)
      Flow Cytometry - Anti-uPA Receptor/U-PAR antibody [EPR22173-257] (ab221680)

      Flow cytometric analysis of U937 (human histiocytic lymphoma cell line) cell line treated with 200 nM TPA for 72 hours (red) or Untreated control (green) labeling uPA Receptor/U-PAR with ab221680 at 1/500 dilution compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

      The expression of uPA Receptor/U-PAR is induced in PMA-stimulated U937 cells (PMID:24999729)

    • Immunoprecipitation - Anti-uPA Receptor/U-PAR antibody [EPR22173-257] (ab221680)
      Immunoprecipitation - Anti-uPA Receptor/U-PAR antibody [EPR22173-257] (ab221680)

      uPA Receptor/U-PAR was immunoprecipitated from 0.35 mg of U937 (human histiocytic lymphoma cell line) treated with 200 nM TPA for 72 hours whole cell lysate with ab221680 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab221680 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

      Lane 1: U937 treated with 200 nM TPA for 72 hours whole cell lysate 10 µg (Input). 

      Lane 2: ab221680 IP in U937 treated with 200 nM TPA for 72 hours whole cell lysate. 

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab221680 in U937 treated with 200 nM TPA for 72 hours whole cell lysate.

      Blocking/Dilution buffer: 5% NFDM/TBST.

      Exposure time: 30 seconds.

      The expression of uPA Receptor/U-PAR is induced in PMA-stimulated U937 cells and the molecular weight is consistent with what has been described in the literature (PMID: 24999729).

    • Anti-uPA Receptor/U-PAR antibody [EPR22173-257] (ab221680)
      Anti-uPA Receptor/U-PAR antibody [EPR22173-257] (ab221680)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab221680? Please let us know so that we can cite the reference in this datasheet.

    ab221680 has not yet been referenced specifically in any publications.

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