Recombinant
RabMAb

Recombinant Anti-uPA Receptor/U-PAR antibody [EPR22173-257] (ab221680)

Overview

  • Product name

    Anti-uPA Receptor/U-PAR antibody [EPR22173-257]
    See all uPA Receptor/U-PAR primary antibodies
  • Description

    Rabbit monoclonal [EPR22173-257] to uPA Receptor/U-PAR
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, Flow Cyt, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human uPA Receptor/U-PAR aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: Q03405

  • Positive control

    • ICC/IF: U937 cells treated with 200 nM TPA for 72 hours. Flow Cyt: U937 cells treated with 200 nM TPA for 72 hours. IP: U937 treated with 200 nM TPA for 72 hours whole cell lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab221680 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.
Flow Cyt 1/500.
IP 1/30.

Target

  • Function

    Acts as a receptor for urokinase plasminogen activator. Plays a role in localizing and promoting plasmin formation. Mediates the proteolysis-independent signal transduction activation effects of U-PA. It is subject to negative-feedback regulation by U-PA which cleaves it into an inactive form.
  • Tissue specificity

    Expressed in neurons of the rolandic area of the brain (at protein level). Expressed in the brain.
  • Sequence similarities

    Contains 3 UPAR/Ly6 domains.
  • Cellular localization

    Secreted and Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD 87 antibody
    • CD87 antibody
    • CD87 antigen antibody
    • MO 3 antibody
    • MO3 antibody
    • Monocyte activation antigen Mo3 antibody
    • Plasminogen activator receptor urokinase antibody
    • Plasminogen activator urokinase receptor antibody
    • PLAUR antibody
    • U PAR antibody
    • u plasminogen activator receptor antibody
    • U-PAR antibody
    • u-plasminogen activator receptor form 2 antibody
    • UPA receptor antibody
    • uPAR antibody
    • UPAR_HUMAN antibody
    • Urinary plasminogen activator receptor antibody
    • URKR antibody
    • Urokinase plasminogen activator receptor antibody
    • Urokinase plasminogen activator surface receptor antibody
    • urokinase-type plasminogen activator (uPA) receptor antibody
    see all

Images

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U937 (human histiocytic lymphoma cell line) cells, untreated or treated with TPA (200 nM, 72 hours), labeling uPA Receptor/U-PAR with ab221680 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    Confocal image showing membranous staining in U937 cells treated with TPA (200 nM, 72 hours).

    The expression of uPA Receptor/U-PAR is induced in PMA-stimulated U937 cells (PMID:24999729).

    The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

  • Flow cytometric analysis of U937 (human histiocytic lymphoma cell line) cell line treated with 200 nM TPA for 72 hours (red) or Untreated control (green) labeling uPA Receptor/U-PAR with ab221680 at 1/500 dilution compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

    The expression of uPA Receptor/U-PAR is induced in PMA-stimulated U937 cells (PMID:24999729)

  • uPA Receptor/U-PAR was immunoprecipitated from 0.35 mg of U937 (human histiocytic lymphoma cell line) treated with 200 nM TPA for 72 hours whole cell lysate with ab221680 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab221680 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

    Lane 1: U937 treated with 200 nM TPA for 72 hours whole cell lysate 10 µg (Input). 

    Lane 2: ab221680 IP in U937 treated with 200 nM TPA for 72 hours whole cell lysate. 

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab221680 in U937 treated with 200 nM TPA for 72 hours whole cell lysate.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: 30 seconds.

    The expression of uPA Receptor/U-PAR is induced in PMA-stimulated U937 cells and the molecular weight is consistent with what has been described in the literature (PMID: 24999729).

References

ab221680 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab221680.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up