Key features and details
- Rabbit polyclonal to UPB1
- Suitable for: IHC-P, WB
- Reacts with: Rat, Human, Pig
- Isotype: IgG
Product nameAnti-UPB1 antibody
See all UPB1 primary antibodies
DescriptionRabbit polyclonal to UPB1
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Rat, Human, Pig
Predicted to work with: Mouse, Orangutan
Recombinant fragment (His-T7-tag) corresponding to Human UPB1 aa 121-384. (Expressed in E.coli).
WTMPFAFCTREKLPWTEFAESAEDGPTTRFCQKLAKNHDMVVVSPILERD SEHGDVLWNTAVVISNSGAVLGKTRKNHIPRVGDFNESTYYMEGNLGHPV FQTQFGRIAVNICYGRHHPLNWLMYSINGAEIIFNPSATIGALSESLWPI EARNAAIANHCFTCAINRVGTEHFPNEFTSGDGKKAHQDFGYFYGSSYVA APDSSRTPGLSRSRDGLLVAKLDLNLCQQVNDVWNFKMTGRYEMYARELA EAVKSNYSPTIVKE
Database link: Q9UBR1
- WB: Recombinant human UPB1 protein; Pig and rat liver lysates. IHC-P: Human kidney, liver and liver cancer tissues.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab231329 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
- Pathways and Processes
- Metabolic signaling pathways
- Nucleotide metabolism
- Purine and pyrimidine synthesis
Our Abpromise guarantee covers the use of ab231329 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
|WB||Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 43 kDa.|
RelevanceUPB1, also known as beta ureidopropionase, belongs to the CN hydrolase family. It catalyzes the last step in the pyrimidine degradation pathway in which the pyrimidine bases uracil and thymine are degraded via the consecutive action of dihydropyrimidine dehydrogenase (DHPDH), dihydropyrimidinase (DHP) and beta-ureidopropionase (UP) to beta-alanine and beta-aminoisobutyric acid, respectively.
- AI195023 antibody
- Beta alanine synthase antibody
- Beta ureidopropionase antibody
Anti-UPB1 antibody (ab231329) at 1 µg/ml + Recombinant human UPB1 protein
HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution
Predicted band size: 43 kDa
All lanes : Anti-UPB1 antibody (ab231329) at 1 µg/ml
Lane 1 : Pig liver lysate
Lane 2 : Rat liver lysate
All lanes : HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution
Predicted band size: 43 kDa
Paraffin-embedded human liver tissue stained for UPB1 using ab231329 at 20 μg/ml in immunohistochemical analysis. DAB staining.
Paraffin-embedded human liver cancer tissue stained for UPB1 using ab231329 at 20 μg/ml in immunohistochemical analysis. DAB staining.
Paraffin-embedded human kidney tissue stained for UPB1 using ab231329 at 20 μg/ml in immunohistochemical analysis. DAB staining.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab231329 has not yet been referenced specifically in any publications.