Overview

  • Product name
    Anti-UPB1 antibody [EPR9132]
    See all UPB1 primary antibodies
  • Description
    Rabbit monoclonal [EPR9132] to UPB1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, IPmore details
    Unsuitable for: ICC
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to residues in Human UPB1 (UniProt: Q9UBR1).

  • Positive control
    • Human fetal liver lysate; Human kidney and liver tissues.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab157195 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 43 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for ICC.
  • Target

    • Relevance
      UPB1, also known as beta ureidopropionase, belongs to the CN hydrolase family. It catalyzes the last step in the pyrimidine degradation pathway in which the pyrimidine bases uracil and thymine are degraded via the consecutive action of dihydropyrimidine dehydrogenase (DHPDH), dihydropyrimidinase (DHP) and beta-ureidopropionase (UP) to beta-alanine and beta-aminoisobutyric acid, respectively.
    • Cellular localization
      Cytoplasmic
    • Database links
    • Alternative names
      • AI195023 antibody
      • Beta alanine synthase antibody
      • Beta ureidopropionase antibody
      • BUP1 antibody
      • N carbamoyl beta alanine amidohydrolase antibody
      • Ureidopropionase, beta antibody
      see all

    Images

    • Anti-UPB1 antibody [EPR9132] (ab157195) at 1/1000 dilution + Human fetal liver lysate at 10 µg

      Secondary
      Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 43 kDa

    • Detection of UPB1 by Western Blot of Immunprecipitate. Human fetal liver lysate immunoprecipitated using ab157195 at 1/10 dilution.
    • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling UPB1 with ab157195 at 1/100 dilution.
    • Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling UPB1 with ab157195 at 1/100 dilution.

    References

    This product has been referenced in:
    • Li B  et al. Development and Validation of a Three-gene Prognostic Signature for Patients with Hepatocellular Carcinoma. Sci Rep 7:5517 (2017). WB, IHC . Read more (PubMed: 28717245) »
    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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