Key features and details
- Rabbit polyclonal to UPD
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-UPD antibody
DescriptionRabbit polyclonal to UPD
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
Recombinant full length protein within Human UPD. The exact sequence is proprietary.
Database link: P06132
Previously labelled as UROD.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.88% Sodium chloride
PBS without Mg2+ and Ca2+.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab196562 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Predicted molecular weight: 41 kDa.|
|ICC/IF||1/50 - 1/200.|
FunctionCatalyzes the decarboxylation of four acetate groups of uroporphyrinogen-III to yield coproporphyrinogen-III.
PathwayPorphyrin metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 4/4.
Involvement in diseaseDefects in UROD are the cause of familial porphyria cutanea tarda (FPCT) [MIM:176100]; also known as porphyria cutanea tarda type II. FPCT is an autosomal dominant disorder characterized by light-sensitive dermatitis, with onset in later life. It is associated with the excretion of large amounts of uroporphyrin in the urine. Iron overload is often present in association with varying degrees of liver damage. Besides the familial form of PCT, a relatively common idiosyncratic form is known in which only the liver enzyme is reduced. This form is referred to as porphyria cutanea tarda "sporadic" type or type I [MIM:176090]. PCT type I occurs sporadically as an unusual accompaniment of common hepatic disorders such as alcohol-associated liver disease.
Defects in UROD are the cause of hepatoerythropoietic porphyria (HEP) [MIM:176100]. HEP is a rare autosomal recessive disorder. It is the severe form of cutaneous porphyria, and presents in infancy. The level of UROD is very low in erythrocytes and cultured skin fibroblasts, suggesting that HEP is the homozygous state for porphyria cutanea tarda.
Sequence similaritiesBelongs to the uroporphyrinogen decarboxylase family.
- Information by UniProt
- DCUP_HUMAN antibody
- PCT antibody
- UPD antibody
All lanes : Anti-UPD antibody (ab196562) at 1/500 dilution
Lane 1 : SW620 cell extract
Lane 2 : K562 cell extract
Lane 3 : SW480 cell extract
Lane 4 : Mouse liver extract
Lane 5 : Mouse kidney extract
Lane 6 : Mouse testis extract
Lane 7 : Mouse heart extract
Lane 8 : HepG2 cell extract
Predicted band size: 41 kDa
Immunofluorescent analysis of U20S cells labeling UPD (red) with ab196562 at 1/50 dilution. Nuclei were counterstained with DAPI (blue).
ab196562 has not yet been referenced specifically in any publications.