Overview

  • Product name

    Anti-UPD antibody
  • Description

    Rabbit polyclonal to UPD
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Recombinant full length protein within Human UPD. The exact sequence is proprietary.
    Database link: P06132

  • Positive control

    • SW620, K562, SW480 and HepG2 cell extracts; mouse liver, kidney, testis and heart extracts; U20S cells
  • General notes

    Previously labelled as UROD. 

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 50% Glycerol, 49% PBS, 0.88% Sodium chloride

    PBS without Mg2+ and Ca2+.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab196562 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 41 kDa.
ICC/IF 1/50 - 1/200.
IHC-P 1/50 - 1/200.

Target

  • Function

    Catalyzes the decarboxylation of four acetate groups of uroporphyrinogen-III to yield coproporphyrinogen-III.
  • Pathway

    Porphyrin metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 4/4.
  • Involvement in disease

    Defects in UROD are the cause of familial porphyria cutanea tarda (FPCT) [MIM:176100]; also known as porphyria cutanea tarda type II. FPCT is an autosomal dominant disorder characterized by light-sensitive dermatitis, with onset in later life. It is associated with the excretion of large amounts of uroporphyrin in the urine. Iron overload is often present in association with varying degrees of liver damage. Besides the familial form of PCT, a relatively common idiosyncratic form is known in which only the liver enzyme is reduced. This form is referred to as porphyria cutanea tarda "sporadic" type or type I [MIM:176090]. PCT type I occurs sporadically as an unusual accompaniment of common hepatic disorders such as alcohol-associated liver disease.
    Defects in UROD are the cause of hepatoerythropoietic porphyria (HEP) [MIM:176100]. HEP is a rare autosomal recessive disorder. It is the severe form of cutaneous porphyria, and presents in infancy. The level of UROD is very low in erythrocytes and cultured skin fibroblasts, suggesting that HEP is the homozygous state for porphyria cutanea tarda.
  • Sequence similarities

    Belongs to the uroporphyrinogen decarboxylase family.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • DCUP_HUMAN antibody
    • PCT antibody
    • UPD antibody
    • URO D antibody
    • URO-D antibody
    • urod antibody
    • Uroporphyrinogen decarboxylase antibody
    • Uroporphyrinogen III decarboxylase antibody
    see all

Images

  • All lanes : Anti-UPD antibody (ab196562) at 1/500 dilution

    Lane 1 : SW620 cell extract
    Lane 2 : K562 cell extract
    Lane 3 : SW480 cell extract
    Lane 4 : Mouse liver extract
    Lane 5 : Mouse kidney extract
    Lane 6 : Mouse testis extract
    Lane 7 : Mouse heart extract
    Lane 8 : HepG2 cell extract

    Predicted band size: 41 kDa

  • Immunofluorescent analysis of U20S cells labeling UPD (red) with ab196562 at 1/50 dilution. Nuclei were counterstained with DAPI (blue).

References

ab196562 has not yet been referenced specifically in any publications.

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