Overview

  • Product name

    Anti-UPF3B/RENT3B antibody
    See all UPF3B/RENT3B primary antibodies
  • Description

    Rabbit polyclonal to UPF3B/RENT3B
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Bat
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 300-350 (KAKKLDKENL SDERASGQSC TLPKRSDSEL KDEKPKRPED ESGRDYRERE R) of Human UPF3B/RENT3B (NP_542199.1)

  • Positive control

    • HeLa, 293T and Jurkat whole cell lysate (ab7899).

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate

    pH 7 to 8.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab134566 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 58 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Relevance

    UPF3B is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. UPF3B binds to the mRNA and remains bound after nuclear export, acting as a nucleocytoplasmic shuttling protein. It forms with Y14 a complex that binds specifically 20 nt upstream of exon-exon junctions. This gene is located on the long arm of chromosome X (referenced from entrez gene).
  • Cellular localization

    Nucleus. Cytoplasm. Note: Shuttling between the nucleus and the cytoplasm.
  • Database links

  • Alternative names

    • HUPF3B antibody
    • hUpf3p X antibody
    • MRXS14 antibody
    • Nonsense mRNA reducing factor 3B antibody
    • Regulator of nonsense transcripts 3B antibody
    • RENT3B antibody
    • Up-frameshift suppressor 3 homolog B antibody
    • Up-frameshift suppressor 3 homolog on chromosome X antibody
    • UPF3 regulator of nonsense transcripts homolog B (yeast) antibody
    • UPF3 regulator of nonsense transcripts homolog B antibody
    • UPF3B antibody
    • UPF3X antibody
    see all

Images

  • All lanes : Anti-UPF3B/RENT3B antibody (ab134566) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : 293T whole cell lysate at 50 µg
    Lane 4 : Jurkat whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 58 kDa


    Exposure time: 10 seconds
  • Detection of UPF3B/RENT3B by Western Blot of Immunprecipitate.
    Immunoprecipitation of HeLa whole cell lysate using ab134566 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane) followed by detection of UPF3B/RENT3B with ab134566 at 1µg/ml. The lysate in lane 2 is precipitated with an IgG control antibody. Detection: Chemiluminescence with exposure time of 3 seconds.

References

This product has been referenced in:

  • Fontaine KA  et al. The Cellular NMD Pathway Restricts Zika Virus Infection and Is Targeted by the Viral Capsid Protein. MBio 9:N/A (2018). Read more (PubMed: 30401782) »
See 1 Publication for this product

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