Key features and details
- Rabbit polyclonal to UPRT
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-UPRT antibody
See all UPRT primary antibodies
DescriptionRabbit polyclonal to UPRT
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Chicken, Cow, Zebrafish, Cynomolgus monkey
- IHC-P: Human stomach tissue. ICC/IF: A431 cells. WB: HEK-293T transfected with human UPRT with C-terminal myc-DDK tag, whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab251653 in the following tested applications.
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 34 kDa.|
Tissue specificityHighly expressed in leukocytes, liver, spleen and thymus, with lower expression in brain, lung and skeletal muscle.
Sequence similaritiesBelongs to the UPRTase family.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- DKFZp781E1243 antibody
- FUR1 antibody
- Gm774 antibody
All lanes : Anti-UPRT antibody (ab251653) at 0.4 µg/ml
Lane 1 : Vector only transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : HEK-293T transfected with human UPRT with C-terminal myc-DDK tag, whole cell lysate.
Predicted band size: 34 kDa
Paraffin-embedded human stomach tissue stained for UPRT using ab251653 at 1/50 dilution in immunohistochemical analysis.
PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for UPRT (green) using ab251653 at 4 µg/ml in ICC/IF.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab251653 has not yet been referenced specifically in any publications.