Anti-UQCRB antibody (ab122827)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-UQCRB antibody
See all UQCRB primary antibodies -
Description
Rabbit polyclonal to UQCRB -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
antigen sequence: LPENLYNDRM FRIKRALDLN LKHQILPKEQ WTKYEEENFY LEPYLKEVIR ERKEREEWAK K, corresponding to internal sequence amino acids 51-111 of Human UQCRB.
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Positive control
- Human colon tissue; RT 4, U 251 MG and Liver lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store undiluted. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab122827 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | 1/250 - 1/500. |
Target
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Function
This is a component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is part of the mitochondrial respiratory chain. This component is involved in redox-linked proton pumping. -
Involvement in disease
Defects in UQCRB are a cause of mitochondrial complex III deficiency (MT-C3D) [MIM:124000]. A disorder of the mitochondrial respiratory chain resulting in a highly variable phenotype depending on which tissues are affected. Clinical features include mitochondrial encephalopathy, psychomotor retardation, ataxia, severe failure to thrive, liver dysfunction, renal tubulopathy, muscle weakness and exercise intolerance. -
Sequence similarities
Belongs to the UQCRB/QCR7 family. -
Cellular localization
Mitochondrion inner membrane. - Information by UniProt
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Database links
- Entrez Gene: 7381 Human
- Entrez Gene: 67530 Mouse
- Entrez Gene: 362897 Rat
- Omim: 191330 Human
- SwissProt: P14927 Human
- SwissProt: Q9D855 Mouse
- Unigene: 131255 Human
- Unigene: 379136 Mouse
see all -
Alternative names
- Complex III subunit 7 antibody
- Complex III subunit VII antibody
- Cytochrome b-c1 complex subunit 7 antibody
see all
Images
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Western blot - Anti-UQCRB antibody (ab122827)All lanes : Anti-UQCRB antibody (ab122827) at 1/250 dilution
Lane 1 : RT 4 lysate
Lane 2 : U 251 MG lysate
Lane 3 : Human plasma lysate
Lane 4 : Human liver lysate
Lane 5 : Human tonsil lysate
Developed using the ECL technique. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRB antibody (ab122827)ab122827 at 1/200 dilution staining UQCRB in Paraffin-embedded Human colon tissue by Immunohistochemistry tissue.
Datasheets and documents
References
This product has been referenced in:
- Sun Y et al. miR-142 controls metabolic reprogramming that regulates dendritic cell activation. J Clin Invest 129:2029-2042 (2019). Read more (PubMed: 30958798) »
