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    uqcrb-antibody-ab122827.pdf

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Cardiovascular Lipids / Lipoproteins Lipid Metabolism Cytochromes
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Anti-UQCRB antibody (ab122827)

  • Datasheet
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Western blot - Anti-UQCRB antibody (ab122827)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRB antibody (ab122827)

Key features and details

  • Rabbit polyclonal to UQCRB
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

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Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-UQCRB antibody
    See all UQCRB primary antibodies
  • Description

    Rabbit polyclonal to UQCRB
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    antigen sequence: LPENLYNDRM FRIKRALDLN LKHQILPKEQ WTKYEEENFY LEPYLKEVIR ERKEREEWAK K, corresponding to internal sequence amino acids 51-111 of Human UQCRB.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • Human colon tissue; RT 4, U 251 MG and Liver lysates.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store undiluted.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Lipids / Lipoproteins
    • Lipid Metabolism
    • Cytochromes
    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Cell Biology
    • Other Antibodies
    • Oxidative Stress
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipases
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Cytochromes

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

Our Abpromise guarantee covers the use of ab122827 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 0.04 - 0.4 µg/ml.

Target

  • Function

    This is a component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is part of the mitochondrial respiratory chain. This component is involved in redox-linked proton pumping.
  • Involvement in disease

    Defects in UQCRB are a cause of mitochondrial complex III deficiency (MT-C3D) [MIM:124000]. A disorder of the mitochondrial respiratory chain resulting in a highly variable phenotype depending on which tissues are affected. Clinical features include mitochondrial encephalopathy, psychomotor retardation, ataxia, severe failure to thrive, liver dysfunction, renal tubulopathy, muscle weakness and exercise intolerance.
  • Sequence similarities

    Belongs to the UQCRB/QCR7 family.
  • Cellular localization

    Mitochondrion inner membrane.
  • Target information above from: UniProt accession P14927 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 7381 Human
    • Entrez Gene: 67530 Mouse
    • Entrez Gene: 362897 Rat
    • Omim: 191330 Human
    • SwissProt: P14927 Human
    • SwissProt: Q9D855 Mouse
    • Unigene: 131255 Human
    • Unigene: 379136 Mouse
    • Unigene: 105517 Rat
    see all
  • Alternative names

    • Complex III subunit 7 antibody
    • Complex III subunit VII antibody
    • Cytochrome b-c1 complex subunit 7 antibody
    • MC3DN3 antibody
    • Mitochondrial ubiquinone-binding protein antibody
    • QCR7 antibody
    • QCR7_HUMAN antibody
    • QP-C antibody
    • QPC antibody
    • Ubiquinol cytochrome c reductase complex III subunit VI antibody
    • Ubiquinol-cytochrome c reductase binding protein antibody
    • Ubiquinol-cytochrome c reductase complex 14 kDa protein antibody
    • Ubiquinone-binding protein antibody
    • UQBC antibody
    • UQBP antibody
    • UQCR6 antibody
    • UQCRB antibody
    • UQPC antibody
    see all

Images

  • Western blot - Anti-UQCRB antibody (ab122827)
    Western blot - Anti-UQCRB antibody (ab122827)
    All lanes : Anti-UQCRB antibody (ab122827) at 1/250 dilution

    Lane 1 : RT 4 lysate
    Lane 2 : U 251 MG lysate
    Lane 3 : Human plasma lysate
    Lane 4 : Human liver lysate
    Lane 5 : Human tonsil lysate

    Developed using the ECL technique.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRB antibody (ab122827)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRB antibody (ab122827)
    ab122827 at 1/200 dilution staining UQCRB in Paraffin-embedded Human colon tissue by Immunohistochemistry tissue.

Protocols

  • Western blot protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (2)

    Publishing research using ab122827? Please let us know so that we can cite the reference in this datasheet.

    ab122827 has been referenced in 2 publications.

    • Timón-Gómez A  et al. Distinct Roles of Mitochondrial HIGD1A and HIGD2A in Respiratory Complex and Supercomplex Biogenesis. Cell Rep 31:107607 (2020). PubMed: 32375044
    • Sun Y  et al. miR-142 controls metabolic reprogramming that regulates dendritic cell activation. J Clin Invest 129:2029-2042 (2019). PubMed: 30958798

    Customer reviews and Q&As

    Show All Reviews Q&A
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