Anti-UQCRB antibody (ab229465)
Key features and details
- Rabbit polyclonal to UQCRB
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-UQCRB antibody
See all UQCRB primary antibodies -
Description
Rabbit polyclonal to UQCRB -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment within Human UQCRB (internal sequence). The exact sequence is proprietary.
Database link: P14927 -
Positive control
- WB: Human and mouse liver tissue extracts. IHC-P: Mouse liver and rat intestine tissues.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab229465 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/100 - 1/1000. | |
| WB | 1/1000 - 1/10000. Predicted molecular weight: 14 kDa. |
Target
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Function
This is a component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is part of the mitochondrial respiratory chain. This component is involved in redox-linked proton pumping. -
Involvement in disease
Defects in UQCRB are a cause of mitochondrial complex III deficiency (MT-C3D) [MIM:124000]. A disorder of the mitochondrial respiratory chain resulting in a highly variable phenotype depending on which tissues are affected. Clinical features include mitochondrial encephalopathy, psychomotor retardation, ataxia, severe failure to thrive, liver dysfunction, renal tubulopathy, muscle weakness and exercise intolerance. -
Sequence similarities
Belongs to the UQCRB/QCR7 family. -
Cellular localization
Mitochondrion inner membrane. - Information by UniProt
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Database links
- Entrez Gene: 7381 Human
- Entrez Gene: 67530 Mouse
- Entrez Gene: 362897 Rat
- Omim: 191330 Human
- SwissProt: P14927 Human
- SwissProt: Q9D855 Mouse
- Unigene: 131255 Human
- Unigene: 379136 Mouse
see all -
Alternative names
- Complex III subunit 7 antibody
- Complex III subunit VII antibody
- Cytochrome b-c1 complex subunit 7 antibody
see all
Images
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Western blot - Anti-UQCRB antibody (ab229465)All lanes : Anti-UQCRB antibody (ab229465) at 1/5000 dilution
Lane 1 : Mouse liver tissue extracts at 50 µg
Lane 2 : Human liver tissue extracts at 30 µg
Predicted band size: 14 kDa15% SDS-PAGE
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRB antibody (ab229465)Paraffin-embedded rat intestine stained for UQCRB using ab229465 at 1/500 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRB antibody (ab229465)Paraffin-embedded mouse liver tissue stained for UQCRB using ab229465 at 1/500 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (0)
ab229465 has not yet been referenced specifically in any publications.
