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RecombinantRabMAb

Recombinant Anti-UQCRB antibody [EPR15591] - BSA and Azide free (ab251022)

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Western blot - Anti-UQCRB antibody [EPR15591] - BSA and Azide free (ab251022)
  • Western blot - Anti-UQCRB antibody [EPR15591] - BSA and Azide free (ab251022)
  • Western blot - Anti-UQCRB antibody [EPR15591] - BSA and Azide free (ab251022)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRB antibody [EPR15591] - BSA and Azide free (ab251022)
  • Immunocytochemistry - Anti-UQCRB antibody [EPR15591] - BSA and Azide free (ab251022)
  • Anti-UQCRB antibody [EPR15591] - BSA and Azide free (ab251022)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15591] to UQCRB - BSA and Azide free
  • Suitable for: ICC, WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Unconjugated

Overview

  • Product name

    Anti-UQCRB antibody [EPR15591] - BSA and Azide free
    See all UQCRB primary antibodies

  • Description

    Rabbit monoclonal [EPR15591] to UQCRB - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab251022 is the carrier-free version of ab190360.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab251022 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Notes
ICC
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    This is a component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is part of the mitochondrial respiratory chain. This component is involved in redox-linked proton pumping.

  • Involvement in disease

    Defects in UQCRB are a cause of mitochondrial complex III deficiency (MT-C3D) [MIM:124000]. A disorder of the mitochondrial respiratory chain resulting in a highly variable phenotype depending on which tissues are affected. Clinical features include mitochondrial encephalopathy, psychomotor retardation, ataxia, severe failure to thrive, liver dysfunction, renal tubulopathy, muscle weakness and exercise intolerance.

  • Sequence similarities

    Belongs to the UQCRB/QCR7 family.

  • Cellular localization

    Mitochondrion inner membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • Complex III subunit 7 antibody
      • Complex III subunit VII antibody
      • Cytochrome b-c1 complex subunit 7 antibody
      • MC3DN3 antibody
      • Mitochondrial ubiquinone-binding protein antibody
      • QCR7 antibody
      • QCR7_HUMAN antibody
      • QP-C antibody
      • QPC antibody
      • Ubiquinol cytochrome c reductase complex III subunit VI antibody
      • Ubiquinol-cytochrome c reductase binding protein antibody
      • Ubiquinol-cytochrome c reductase complex 14 kDa protein antibody
      • Ubiquinone-binding protein antibody
      • UQBC antibody
      • UQBP antibody
      • UQCR6 antibody
      • UQCRB antibody
      • UQPC antibody
      see all

    Images

    • Western blot - Anti-UQCRB antibody [EPR15591] - BSA and Azide free (ab251022)
      Western blot - Anti-UQCRB antibody [EPR15591] - BSA and Azide free (ab251022)
      All lanes : Anti-UQCRB antibody [EPR15591] (ab190360) at 1/20000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : Human fetal heart tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 14 kDa
      Observed band size: 14 kDa



      This data was developed using ab190360, the same antibody clone in a different buffer formulation.

    • Western blot - Anti-UQCRB antibody [EPR15591] - BSA and Azide free (ab251022)
      Western blot - Anti-UQCRB antibody [EPR15591] - BSA and Azide free (ab251022)
      Anti-UQCRB antibody [EPR15591] (ab190360) at 1/5000 dilution + 293 cell lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 14 kDa
      Observed band size: 14 kDa



      This data was developed using ab190360, the same antibody clone in a different buffer formulation.

    • Western blot - Anti-UQCRB antibody [EPR15591] - BSA and Azide free (ab251022)
      Western blot - Anti-UQCRB antibody [EPR15591] - BSA and Azide free (ab251022)
      All lanes : Anti-UQCRB antibody [EPR15591] (ab190360) at 1/1000 dilution

      Lane 1 : Mouse heart tissue lysate
      Lane 2 : Rat brain tissue lysate
      Lane 3 : NIH/3T3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 14 kDa



      This data was developed using ab190360, the same antibody clone in a different buffer formulation.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRB antibody [EPR15591] - BSA and Azide free (ab251022)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRB antibody [EPR15591] - BSA and Azide free (ab251022)
      This data was developed using ab190360, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human adenocarcinoma of the colon labeling UQCRB with ab190360 at 1/100 and HRP Polymer for Rabbit IgG. Counterstained with hematoxylin. Negative control also shown. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
    • Immunocytochemistry - Anti-UQCRB antibody [EPR15591] - BSA and Azide free (ab251022)
      Immunocytochemistry - Anti-UQCRB antibody [EPR15591] - BSA and Azide free (ab251022)
      This data was developed using ab190360, the same antibody clone in a different buffer formulation.Immunocytochemical analysis of HeLa cells fixed in 4% paraformaldehyde labeling UQCRB with ab190360 at 1/250 dilution and Goat anti rabbit IgG (Alexa Fluor® 555) at 1/200 dilution. Counterstained with DAPI. Negative controls also shown.
    • Anti-UQCRB antibody [EPR15591] - BSA and Azide free (ab251022)
      Anti-UQCRB antibody [EPR15591] - BSA and Azide free (ab251022)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab251022? Please let us know so that we can cite the reference in this datasheet.

    ab251022 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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