Key features and details
- Rabbit polyclonal to UQCRC1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-UQCRC1 antibody
See all UQCRC1 primary antibodies
DescriptionRabbit polyclonal to UQCRC1
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
- IHC-P: Human small intestine tissue. WB: HEK-293, NIH/3T3, NBT-II and PC-12 cell lysates.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab223746 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 53 kDa.|
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionThis is a component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is part of the mitochondrial respiratory chain. This protein may mediate formation of the complex between cytochromes c and c1.
Sequence similaritiesBelongs to the peptidase M16 family. UQCRC1/QCR1 subfamily.
Cellular localizationMitochondrion inner membrane.
- Information by UniProt
- Complex III subunit 1 antibody
- Core protein I antibody
- Cytochrome b-c1 complex subunit 1 antibody
Paraffin-embedded human heart muscle tissue stained for UQCRC1 using ab223746 at 1/200 dilution in immunohistochemical analysis.
Paraffin-embedded human lymph node tissue stained for UQCRC1 using ab223746 at 1/200 dilution in immunohistochemical analysis.
Paraffin-embedded human small intestine tissue stained for UQCRC1 using ab223746 at 1/200 dilution in immunohistochemical analysis.
Anti-UQCRC1 antibody (ab223746) at 1/100 dilution + HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Predicted band size: 53 kDa
All lanes : Anti-UQCRC1 antibody (ab223746) at 1/100 dilution
Lane 1 : NIH/3T3 (mouse embryo fibroblast cell line) cell lysate
Lane 2 : NBT-II cell lysate
Lane 3 : PC-12 (rat adrenal gland pheochromocytoma cell line) cell lysate
Predicted band size: 53 kDa
ab223746 has not yet been referenced specifically in any publications.