Anti-UQCRC2 antibody [13G12AF12BB11] (Alexa Fluor® 488) (ab197953)

Overview

  • Product name

    Anti-UQCRC2 antibody [13G12AF12BB11] (Alexa Fluor® 488)
    See all UQCRC2 primary antibodies
  • Description

    Mouse monoclonal [13G12AF12BB11] to UQCRC2 (Alexa Fluor® 488)
  • Host species

    Mouse
  • Conjugation

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm
  • Tested applications

    Suitable for: Flow Cyt, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Tissue, cells or virus corresponding to Cow UQCRC2.

  • Positive control

    • ICC/IF: MEF1 cells. Flow Cyt: HepG2 cells.
  • General notes

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties

Applications

Our Abpromise guarantee covers the use of ab197953 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/500.
ICC/IF 1/100.

This product gave a positive signal in MEF1 cells fixed with 100% methanol (5 min).

Target

  • Function

    This is a component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is part of the mitochondrial respiratory chain. The core protein 2 is required for the assembly of the complex.
  • Sequence similarities

    Belongs to the peptidase M16 family. UQCRC2/QCR2 subfamily.
  • Cellular localization

    Mitochondrion inner membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Complex III subunit 2 antibody
    • Core protein II antibody
    • Cytochrome b c1 complex subunit 2 mitochondrial antibody
    • Cytochrome b-c1 complex subunit 2 antibody
    • EC 1.10.2.2 antibody
    • MC3DN5 antibody
    • mitochondrial antibody
    • QCR2 antibody
    • QCR2_HUMAN antibody
    • Ubiquinol cytochrome c reductase complex core protein 2 mitochondrial antibody
    • Ubiquinol cytochrome c reductase core protein II antibody
    • Ubiquinol-cytochrome-c reductase complex core protein 2 antibody
    • UQCR2 antibody
    • Uqcrc2 antibody
    see all

Images

  • ab197953 staining UQCRC2 in MEF1 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab197953 at a 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at a 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

References

ab197953 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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