Anti-UQCRC2 antibody (ab103616)

Rabbit polyclonal UQCRC2 antibody. Validated in WB, ICC/IF and tested in Mouse, Human. Cited in 2 publication(s). Immunogen corresponding to full length protein.

Overview

  • Product name

  • Description

    Rabbit polyclonal to UQCRC2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Full length Human UQCRC2 (NP_003357.2, amino acids 1-453).

  • Positive control

    • Human colon and Mouse kidney tissue lysates; A431 cell lysate; UQCRC2 transfected 293T cell lysate This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HeLa

Applications

Our Abpromise guarantee covers the use of ab103616 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 48 kDa.
ICC/IF Use a concentration of 5 µg/ml.

Target

  • Function

    This is a component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is part of the mitochondrial respiratory chain. The core protein 2 is required for the assembly of the complex.
  • Sequence similarities

    Belongs to the peptidase M16 family. UQCRC2/QCR2 subfamily.
  • Cellular localization

    Mitochondrion inner membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Complex III subunit 2 antibody
    • Core protein II antibody
    • Cytochrome b c1 complex subunit 2 mitochondrial antibody
    • Cytochrome b-c1 complex subunit 2 antibody
    • EC 1.10.2.2 antibody
    • MC3DN5 antibody
    • mitochondrial antibody
    • QCR2 antibody
    • QCR2_HUMAN antibody
    • Ubiquinol cytochrome c reductase complex core protein 2 mitochondrial antibody
    • Ubiquinol cytochrome c reductase core protein II antibody
    • Ubiquinol-cytochrome-c reductase complex core protein 2 antibody
    • UQCR2 antibody
    • Uqcrc2 antibody
    see all

Images

  • ICC/IF image of ab103616 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab103616 at 5µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • Anti-UQCRC2 antibody (ab103616) at 5 µg/ml + Human colon tissue lysate at 50 µg

    Predicted band size: 48 kDa

  • Anti-UQCRC2 antibody (ab103616) at 5 µg/ml + Mouse kidney tissue lysate at 50 µg

    Predicted band size: 48 kDa

  • Anti-UQCRC2 antibody (ab103616) at 5 µg/ml + A431 cell lysate at 50 µg

    Predicted band size: 48 kDa

  • All lanes : Anti-UQCRC2 antibody (ab103616) at 5 µg/ml

    Lane 1 : UQCRC2 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Predicted band size: 48 kDa

References

This product has been referenced in:

  • Wang Y  et al. The STAT-ROS cycle extends IFN-induced cancer cell apoptosis. Int J Oncol 52:305-313 (2018). Read more (PubMed: 29115415) »
  • Basisty N  et al. Mitochondrial-targeted catalase is good for the old mouse proteome, but not for the young: 'reverse' antagonistic pleiotropy? Aging Cell 15:634-45 (2016). WB ; Mouse . Read more (PubMed: 27061426) »
See all 3 Publications for this product

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