Key features and details
- Rabbit polyclonal to UQCRC2
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-UQCRC2 antibody
See all UQCRC2 primary antibodies
DescriptionRabbit polyclonal to UQCRC2
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
- HeLa, 293T, Jurkat, TCMK1 and NIH 3T3 cell lysate.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab217128 was affinity purified using an epitope specific to UQCRC2 immobilized on solid support.
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Integration of energy
Our Abpromise guarantee covers the use of ab217128 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 48 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionThis is a component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is part of the mitochondrial respiratory chain. The core protein 2 is required for the assembly of the complex.
Sequence similaritiesBelongs to the peptidase M16 family. UQCRC2/QCR2 subfamily.
Cellular localizationMitochondrion inner membrane.
- Information by UniProt
- Complex III subunit 2 antibody
- Core protein II antibody
- Cytochrome b c1 complex subunit 2 mitochondrial antibody
All lanes : Anti-DPP3 antibody (ab217127) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : TCMK1 whole cell lysate
Lane 5 : NIH 3T3 whole cell lysate
Lysates/proteins at 15 µg per lane.
Developed using the ECL technique.
Predicted band size: 48 kDa
Exposure time: 3 minutes
NETN lysis buffer was used to prepare the lysates.
Detection of UQCRC2 in Immunoprecipitates of 293T whole cell lysates (1 mg for IP, 20% of IP loaded) using ab217128 at 6 µg/mg lysate for IP (Lane 1). For WB detection ab217128 was used at 1µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
ab217128 has not yet been referenced specifically in any publications.