Overview

  • Product name

  • Description

    Rabbit polyclonal to UQCRC2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human UQCRC2 aa 1-50. The exact sequence is proprietary.
    Sequence:

    MKLLTRAGSF SRFYSLKVAP KVKATAAPAG APPQPQDLEF TKLPNGLVIA


    Database link: P22695

  • Positive control

    • HeLa, 293T, Jurkat, TCMK1 and NIH 3T3 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab217128 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 48 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function

    This is a component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is part of the mitochondrial respiratory chain. The core protein 2 is required for the assembly of the complex.
  • Sequence similarities

    Belongs to the peptidase M16 family. UQCRC2/QCR2 subfamily.
  • Cellular localization

    Mitochondrion inner membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Complex III subunit 2 antibody
    • Core protein II antibody
    • Cytochrome b c1 complex subunit 2 mitochondrial antibody
    • Cytochrome b-c1 complex subunit 2 antibody
    • EC 1.10.2.2 antibody
    • MC3DN5 antibody
    • mitochondrial antibody
    • QCR2 antibody
    • QCR2_HUMAN antibody
    • Ubiquinol cytochrome c reductase complex core protein 2 mitochondrial antibody
    • Ubiquinol cytochrome c reductase core protein II antibody
    • Ubiquinol-cytochrome-c reductase complex core protein 2 antibody
    • UQCR2 antibody
    • Uqcrc2 antibody
    see all

Images

  • All lanes : Anti-DPP3 antibody (ab217127) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate
    Lane 2 : 293T whole cell lysate
    Lane 3 : Jurkat whole cell lysate
    Lane 4 : TCMK1 whole cell lysate
    Lane 5 : NIH 3T3 whole cell lysate

    Lysates/proteins at 15 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 48 kDa


    Exposure time: 3 minutes


    NETN lysis buffer was used to prepare the lysates.

  • Detection of UQCRC2 in Immunoprecipitates of 293T whole cell lysates (1 mg for IP, 20% of IP loaded) using ab217128 at 6 µg/mg lysate for IP (Lane 1). For WB detection ab217128 was used at 1µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

References

ab217128 has not yet been referenced specifically in any publications.

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