Recombinant Anti-UQCRFS1/RISP antibody [EPR16288] (ab191078)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16288] to UQCRFS1/RISP
- Suitable for: WB, IP, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-UQCRFS1/RISP antibody [EPR16288]
See all UQCRFS1/RISP primary antibodies -
Description
Rabbit monoclonal [EPR16288] to UQCRFS1/RISP -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HeLa, Raji, 293, Human fetal heart, mouse brain, mouse kidney, mouse heart, rat brain, rat heart, rat kidney, RAW264.7 and NIH3T3 lysates; mouse stomach, Human ovarian adenocarcinoma and Human kidney tissues; HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16288 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
- HeLa whole cell lysate (ab150035)
- Mouse kidney normal tissue lysate - total protein (ab29305)
- HeLa whole cell lysate (ab29545)
- Raji whole cell lysate (ab30124)
- Mouse brain tissue lysate - total protein (ab30151)
- Mouse heart normal tissue lysate - total protein (ab30291)
- NIH/3T3 whole cell lysate (ab7179)
- RAW 264.7 whole cell lysate (ab7187)
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab191078 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 25 kDa (predicted molecular weight: 30 kDa).
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IP |
1/60.
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ICC/IF |
1/50.
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IHC-P |
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 25 kDa (predicted molecular weight: 30 kDa). |
IP
1/60. |
ICC/IF
1/50. |
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis.
The transit peptide of the Rieske protein seems to form part of the bc1 complex and is considered to be the subunit 11/IX of that complex. -
Sequence similarities
Contains 1 Rieske domain. -
Cellular localization
Mitochondrion inner membrane. - Information by UniProt
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Database links
- Entrez Gene: 7386 Human
- Entrez Gene: 66694 Mouse
- Entrez Gene: 291103 Rat
- Omim: 191327 Human
- SwissProt: P47985 Human
- SwissProt: Q9CR68 Mouse
- SwissProt: P20788 Rat
- Unigene: 726119 Human
see all -
Alternative names
- Complex III subunit 5 antibody
- Complex III subunit IX antibody
- Cytochrome b c1 complex subunit Rieske mitochondrial antibody
see all
Images
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All lanes : Anti-UQCRFS1/RISP antibody [EPR16288] (ab191078) at 1/10000 dilution
Lane 1 : HeLa lysate
Lane 2 : Raji lysates
Lane 3 : 293 lysates
Lane 4 : Human fetal heart lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 30 kDa
Observed band size: 25 kDa why is the actual band size different from the predicted? -
All lanes : Anti-UQCRFS1/RISP antibody [EPR16288] (ab191078) at 1/1000 dilution
Lane 1 : mouse brain lysate
Lane 2 : mouse heart lysate
Lane 3 : mouse kidney lysate
Lane 4 : rat brain lysate
Lane 5 : rat heart lysate
Lane 6 : rat kidney lysate
Lane 7 : RAW264.7 lysate
Lane 8 : NIH3T3 lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 30 kDa
Observed band size: 25 kDa why is the actual band size different from the predicted? -
Western blot analysis of UQCRFS1/RISP in immunoprecipitation pellets from Human fetal lysate. ab191078 used at 1/60 dilution (Lane 1) or PBS (Lane 2). Secondary antibody Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at a1/1500 dilution.
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Immunofluorescent analysis of methanol-fixed HeLa cells labeling UQCRFS1/RISP with ab191078 at 1/50, Goat anti rabbit IgG (Alexa Fluor® 488) at 1/200 and DAPI staining (blue). Negative controls also shown.
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Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling UQCRFS1/RISP with ab191078 at 1/500. Secondary antibody Goat anti rabbit IgG (HRP) prediluted. Counter stain Hematoxylin. Negative control also shown.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human ovarian adenocarcinoma tissue labeling UQCRFS1/RISP with ab191078 at 1/500. Secondary antibody Goat anti rabbit IgG (HRP) prediluted. Counter stain Hematoxylin. Negative control also shown.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Mouse stomach tissue labeling UQCRFS1/RISP with ab191078 at 1/500. Secondary antibody Goat anti rabbit IgG (HRP) prediluted. Counter stain Hematoxylin. Negative control also shown.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (4)
ab191078 has been referenced in 4 publications.
- Maio N et al. Disruption of cellular iron homeostasis by IREB2 missense variants causes severe neurodevelopmental delay, dystonia and seizures. Brain Commun 4:fcac102 (2022). PubMed: 35602653
- Tong WH et al. Hyperactivation of mTOR and AKT in a cardiac hypertrophy animal model of Friedreich ataxia. Heliyon 8:e10371 (2022). PubMed: 36061025
- Li Y et al. CISD3 inhibition drives cystine-deprivation induced ferroptosis. Cell Death Dis 12:839 (2021). PubMed: 34497268
- Maio N et al. A Single Adaptable Cochaperone-Scaffold Complex Delivers Nascent Iron-Sulfur Clusters to Mammalian Respiratory Chain Complexes I-III. Cell Metab 25:945-953.e6 (2017). PubMed: 28380382