Overview

  • Product name

    Anti-UQCRFS1/RISP antibody [EPR16288]
    See all UQCRFS1/RISP primary antibodies
  • Description

    Rabbit monoclonal [EPR16288] to UQCRFS1/RISP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human UQCRFS1/RISP aa 50-150. The exact sequence is proprietary.
    Database link: Q69BK6

  • Positive control

    • HeLa, Raji, 293, Human fetal heart, mouse brain, mouse kidney, mouse heart, rat brain, rat heart, rat kidney, RAW264.7 and NIH3T3 lysates; mouse stomach, Human ovarian adenocarcinoma and Human kidney tissues; HeLa cells.
  • General notes

    Previously labelled as UQCRFS1.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Applications

Our Abpromise guarantee covers the use of ab191078 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 25 kDa (predicted molecular weight: 30 kDa).
IP 1/60.
ICC/IF 1/50.
IHC-P 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis.
    The transit peptide of the Rieske protein seems to form part of the bc1 complex and is considered to be the subunit 11/IX of that complex.
  • Sequence similarities

    Contains 1 Rieske domain.
  • Cellular localization

    Mitochondrion inner membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Complex III subunit 5 antibody
    • Complex III subunit IX antibody
    • Cytochrome b c1 complex subunit Rieske mitochondrial antibody
    • Cytochrome b-c1 complex subunit 11 antibody
    • Cytochrome b-c1 complex subunit 5 antibody
    • Cytochrome b6f complex iron sulfur subunit, chloroplastic antibody
    • petC antibody
    • PGR1 antibody
    • Plastohydroquinone:plastocyanin oxidoreductase iron sulfur protein antibody
    • Proton gradient regulation protein 1 antibody
    • Rieske iron sulfur protein antibody
    • Rieske iron-sulfur protein antibody
    • RIP1 antibody
    • RIS1 antibody
    • RISP antibody
    • Ubiquinol cytochrome C reductase rieske iron sulphur antibody
    • Ubiquinol cytochrome c reductase Rieske iron-sulfur polypeptide 1 antibody
    • Ubiquinol-cytochrome c reductase 8 kDa protein antibody
    • Ubiquinol-cytochrome c reductase iron-sulfur subunit antibody
    • UCRI_HUMAN antibody
    • UQCR5 antibody
    • UQCRFS1 antibody
    see all

Images

  • All lanes : Anti-UQCRFS1/RISP antibody [EPR16288] (ab191078) at 1/10000 dilution

    Lane 1 : HeLa lysate
    Lane 2 : Raji lysates
    Lane 3 : 293 lysates
    Lane 4 : Human fetal heart lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 30 kDa
    Observed band size: 25 kDa
    why is the actual band size different from the predicted?

  • All lanes : Anti-UQCRFS1/RISP antibody [EPR16288] (ab191078) at 1/1000 dilution

    Lane 1 : mouse brain lysate
    Lane 2 : mouse heart lysate
    Lane 3 : mouse kidney lysate
    Lane 4 : rat brain lysate
    Lane 5 : rat heart lysate
    Lane 6 : rat kidney lysate
    Lane 7 : RAW264.7 lysate
    Lane 8 : NIH3T3 lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 30 kDa
    Observed band size: 25 kDa why is the actual band size different from the predicted?

  • Western blot analysis of UQCRFS1/RISP in immunoprecipitation pellets from Human fetal lysate. ab191078 used at 1/60 dilution (Lane 1) or PBS (Lane 2). Secondary antibody Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at a1/1500 dilution.

  • Immunofluorescent analysis of methanol-fixed HeLa cells labeling UQCRFS1/RISP with ab191078 at 1/50, Goat anti rabbit IgG (Alexa Fluor® 488) at 1/200 and DAPI staining (blue). Negative controls also shown.

  • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling UQCRFS1/RISP with ab191078 at 1/500. Secondary antibody Goat anti rabbit IgG (HRP) prediluted. Counter stain Hematoxylin. Negative control also shown.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded Human ovarian adenocarcinoma tissue labeling UQCRFS1/RISP with ab191078 at 1/500. Secondary antibody Goat anti rabbit IgG (HRP) prediluted. Counter stain Hematoxylin. Negative control also shown.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded Mouse stomach tissue labeling UQCRFS1/RISP with ab191078 at 1/500. Secondary antibody Goat anti rabbit IgG (HRP) prediluted. Counter stain Hematoxylin. Negative control also shown.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

References

This product has been referenced in:

  • Maio N  et al. A Single Adaptable Cochaperone-Scaffold Complex Delivers Nascent Iron-Sulfur Clusters to Mammalian Respiratory Chain Complexes I-III. Cell Metab 25:945-953.e6 (2017). Read more (PubMed: 28380382) »
See 1 Publication for this product

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