RecombinantRabMAb

Recombinant Anti-UQCRFS1/RISP antibody [EPR16288] - BSA and Azide free (ab251053)

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Western blot - Anti-UQCRFS1/RISP antibody [EPR16288] - BSA and Azide free (ab251053)
  • Western blot - Anti-UQCRFS1/RISP antibody [EPR16288] - BSA and Azide free (ab251053)
  • Immunoprecipitation - Anti-UQCRFS1/RISP antibody [EPR16288] - BSA and Azide free (ab251053)
  • Immunocytochemistry - Anti-UQCRFS1/RISP antibody [EPR16288] - BSA and Azide free (ab251053)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRFS1/RISP antibody [EPR16288] - BSA and Azide free (ab251053)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRFS1/RISP antibody [EPR16288] - BSA and Azide free (ab251053)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRFS1/RISP antibody [EPR16288] - BSA and Azide free (ab251053)
  • Anti-UQCRFS1/RISP antibody [EPR16288] - BSA and Azide free (ab251053)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16288] to UQCRFS1/RISP - BSA and Azide free
  • Suitable for: ICC, IP, WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-UQCRFS1/RISP antibody [EPR16288] - BSA and Azide free
    See all UQCRFS1/RISP primary antibodies

  • Description

    Rabbit monoclonal [EPR16288] to UQCRFS1/RISP - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, IP, WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251053 is the carrier-free version of ab191078. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab251053 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab251053 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 25 kDa (predicted molecular weight: 30 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Notes
ICC
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 25 kDa (predicted molecular weight: 30 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis.
    The transit peptide of the Rieske protein seems to form part of the bc1 complex and is considered to be the subunit 11/IX of that complex.

  • Sequence similarities

    Contains 1 Rieske domain.

  • Cellular localization

    Mitochondrion inner membrane.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Complex III subunit 5 antibody
    • Complex III subunit IX antibody
    • Cytochrome b c1 complex subunit Rieske mitochondrial antibody
    • Cytochrome b-c1 complex subunit 11 antibody
    • Cytochrome b-c1 complex subunit 5 antibody
    • Cytochrome b6f complex iron sulfur subunit, chloroplastic antibody
    • petC antibody
    • PGR1 antibody
    • Plastohydroquinone:plastocyanin oxidoreductase iron sulfur protein antibody
    • Proton gradient regulation protein 1 antibody
    • Rieske iron sulfur protein antibody
    • Rieske iron-sulfur protein antibody
    • RIP1 antibody
    • RIS1 antibody
    • RISP antibody
    • Ubiquinol cytochrome C reductase rieske iron sulphur antibody
    • Ubiquinol cytochrome c reductase Rieske iron-sulfur polypeptide 1 antibody
    • Ubiquinol-cytochrome c reductase 8 kDa protein antibody
    • Ubiquinol-cytochrome c reductase iron-sulfur subunit antibody
    • UCRI_HUMAN antibody
    • UQCR5 antibody
    • UQCRFS1 antibody
    see all

Images

  • Western blot - Anti-UQCRFS1/RISP antibody [EPR16288] - BSA and Azide free (ab251053)
    Western blot - Anti-UQCRFS1/RISP antibody [EPR16288] - BSA and Azide free (ab251053)
    All lanes : Anti-UQCRFS1/RISP antibody [EPR16288] (ab191078) at 1/10000 dilution

    Lane 1 : HeLa lysate
    Lane 2 : Raji lysates
    Lane 3 : 293 lysates
    Lane 4 : Human fetal heart lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 30 kDa
    Observed band size: 25 kDa why is the actual band size different from the predicted?



    This data was developed using ab191078, the same antibody clone in a different buffer formulation.

  • Western blot - Anti-UQCRFS1/RISP antibody [EPR16288] - BSA and Azide free (ab251053)
    Western blot - Anti-UQCRFS1/RISP antibody [EPR16288] - BSA and Azide free (ab251053)
    All lanes : Anti-UQCRFS1/RISP antibody [EPR16288] (ab191078) at 1/1000 dilution

    Lane 1 : mouse brain lysate
    Lane 2 : mouse heart lysate
    Lane 5 : rat heart lysate
    Lane 6 : rat kidney lysate
    Lane 7 : RAW264.7 lysate
    Lane 8 : NIH3T3 lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 30 kDa
    Observed band size: 25 kDa why is the actual band size different from the predicted?



    This data was developed using ab191078, the same antibody clone in a different buffer formulation.

  • Immunoprecipitation - Anti-UQCRFS1/RISP antibody [EPR16288] - BSA and Azide free (ab251053)
    Immunoprecipitation - Anti-UQCRFS1/RISP antibody [EPR16288] - BSA and Azide free (ab251053)

    This data was developed using ab191078, the same antibody clone in a different buffer formulation.

    Western blot analysis of UQCRFS1/RISP in immunoprecipitation pellets from Human fetal lysate. ab191078 used at 1/60 dilution (Lane 1) or PBS (Lane 2). Secondary antibody Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at a1/1500 dilution.

  • Immunocytochemistry - Anti-UQCRFS1/RISP antibody [EPR16288] - BSA and Azide free (ab251053)
    Immunocytochemistry - Anti-UQCRFS1/RISP antibody [EPR16288] - BSA and Azide free (ab251053)
    This data was developed using ab191078, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of methanol-fixed HeLa cells labeling UQCRFS1/RISP with ab191078 at 1/50, Goat anti rabbit IgG (Alexa Fluor® 488) at 1/200 and DAPI staining (blue). Negative controls also shown.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRFS1/RISP antibody [EPR16288] - BSA and Azide free (ab251053)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRFS1/RISP antibody [EPR16288] - BSA and Azide free (ab251053)
    This data was developed using ab191078, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling UQCRFS1/RISP with ab191078 at 1/500. Secondary antibody Goat anti rabbit IgG (HRP) prediluted. Counter stain Hematoxylin. Negative control also shown. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRFS1/RISP antibody [EPR16288] - BSA and Azide free (ab251053)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRFS1/RISP antibody [EPR16288] - BSA and Azide free (ab251053)
    This data was developed using ab191078, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human ovarian adenocarcinoma tissue labeling UQCRFS1/RISP with ab191078 at 1/500. Secondary antibody Goat anti rabbit IgG (HRP) prediluted. Counter stain Hematoxylin. Negative control also shown. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRFS1/RISP antibody [EPR16288] - BSA and Azide free (ab251053)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRFS1/RISP antibody [EPR16288] - BSA and Azide free (ab251053)
    This data was developed using ab191078, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Mouse stomach tissue labeling UQCRFS1/RISP with ab191078 at 1/500. Secondary antibody Goat anti rabbit IgG (HRP) prediluted. Counter stain Hematoxylin. Negative control also shown. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Anti-UQCRFS1/RISP antibody [EPR16288] - BSA and Azide free (ab251053)
    Anti-UQCRFS1/RISP antibody [EPR16288] - BSA and Azide free (ab251053)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab251053? Please let us know so that we can cite the reference in this datasheet.

ab251053 has not yet been referenced specifically in any publications.

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