Recombinant Anti-UQCRFS1/RISP antibody [EPR16289] - BSA and Azide free (ab251054)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16289] to UQCRFS1/RISP - BSA and Azide free
- Suitable for: Flow Cyt (Intra), IP, WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-UQCRFS1/RISP antibody [EPR16289] - BSA and Azide free
See all UQCRFS1/RISP primary antibodies -
Description
Rabbit monoclonal [EPR16289] to UQCRFS1/RISP - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), IP, WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab251054 is the carrier-free version of ab191079.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Clonality
Monoclonal -
Clone number
EPR16289 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab251054 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
Use at an assay dependent concentration.
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IP |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Detects a band of approximately 25 kDa (predicted molecular weight: 30 kDa).
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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ICC/IF |
Use at an assay dependent concentration.
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Notes |
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Flow Cyt (Intra)
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Detects a band of approximately 25 kDa (predicted molecular weight: 30 kDa). |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
Use at an assay dependent concentration. |
Target
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Function
Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis.
The transit peptide of the Rieske protein seems to form part of the bc1 complex and is considered to be the subunit 11/IX of that complex. -
Sequence similarities
Contains 1 Rieske domain. -
Cellular localization
Mitochondrion inner membrane. - Information by UniProt
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Database links
- Entrez Gene: 7386 Human
- Entrez Gene: 66694 Mouse
- Entrez Gene: 291103 Rat
- Omim: 191327 Human
- SwissProt: P47985 Human
- SwissProt: Q9CR68 Mouse
- SwissProt: P20788 Rat
- Unigene: 726119 Human
see all -
Alternative names
- Complex III subunit 5 antibody
- Complex III subunit IX antibody
- Cytochrome b c1 complex subunit Rieske mitochondrial antibody
see all
Images
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All lanes : Anti-UQCRFS1/RISP antibody [EPR16289] - C-terminal (ab191079) at 1/1000 dilution
Lane 1 : PC12 cell lysate
Lanes 2-3 : Mouse brain lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugate at 1/1000 dilution
Predicted band size: 30 kDaThis data was developed using ab191079, the same antibody clone in a different buffer formulation.
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This data was developed using ab191079, the same antibody clone in a different buffer formulation.Western blot analysis of immunoprecipitation pellet from Human fetal heart lysate immunoprecipitated using ab191079 at 1/90 dilution (lane 1) or PBS control (lane 2).
Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution. -
This data was developed using ab191079, the same antibody clone in a different buffer formulation.
Intracellular flow cytometric analysis of HeLa cells (paraformaldehyde-fixed, 2%) labeling UQCRFS1/RISP with ab191079 at 1/280 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
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This data was developed using ab191079, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of HepG2 cells (4% Paraformaldehyde-fixed; 0.1% tritonX-100 permeabilized) labeling UQCRFS1/RISP with ab191079 at 1/100 dilution followed by Goat anti rabbit IgG (AlexaFluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue). Negative controls also shown.
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This data was developed using ab191079, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling UQCRFS1/RISP with ab191079 at 1/100 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin (inset: negative control). Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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All lanes : Anti-UQCRFS1/RISP antibody [EPR16289] - C-terminal (ab191079) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Raji cell lysate
Lane 3 : 293 cell lysate
Lane 4 : Human fetal heart lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugate at 1/1000 dilution
Predicted band size: 30 kDaThis data was developed using ab191079, the same antibody clone in a different buffer formulation.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab251054 has not yet been referenced specifically in any publications.