Recombinant
RabMAb

Recombinant Anti-UQCRH antibody [EPR9038(B)] (ab154803)

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Overview

  • Product name

    Anti-UQCRH antibody [EPR9038(B)]
    See all UQCRH primary antibodies

  • Description

    Rabbit monoclonal [EPR9038(B)] to UQCRH

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, Flow Cyt, WB, IHC-Pmore details
    Unsuitable for: ICC/IF

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human UQCRH. The exact sequence is proprietary.

  • Positive control

    • Fetal liver, PC12, C6, fetal brain, Human spleen and Saos 2 lysates; Human colon and liver tissues; Saos 2 cells.

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab154803 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/10 - 1/100.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
WB 1/1000 - 1/10000. Predicted molecular weight: 11 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for ICC/IF.

    • Target

    • Relevance

      UQRCH is a component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is part of the mitochondrial respiratory chain. It may play a role in electron transfer between cytochromes c1 and c.

    • Cellular localization

      Mitochondrial. Mitochondrion inner membrane.

    • Database links

      see all

    • Alternative names

      • Complex III subunit 6 antibody
      • Complex III subunit VIII antibody
      • Cytochrome b c1 complex subunit 6 antibody
      • Cytochrome b c1 complex subunit 6 mitochondrial antibody
      • Cytochrome c1 non heme 11 kDa protein antibody
      • High protein , mitochondrial antibody
      • MGC111572 antibody
      • Mitochondrial hinge protein antibody
      • QCR 6 antibody
      • QCR6 antibody
      • Ubiquinol cytochrome c reductase complex 11 kDa protein antibody
      • Ubiquinol cytochrome c reductase complex 11 kDa protein mitochondrial antibody
      • Ubiquinol cytochrome c reductase complex III subunit VIII antibody
      • Ubiquinol cytochrome c reductase hinge protein antibody
      • ubiquinol-cytochrome c reductase hinge protein antibody
      • UQCR8 antibody
      see all

    Images

    • Western blot - Anti-UQCRH antibody [EPR9038(B)] (ab154803)
      Western blot - Anti-UQCRH antibody [EPR9038(B)] (ab154803)
      All lanes : Anti-UQCRH antibody [EPR9038(B)] (ab154803) at 1/1000 dilution

      Lane 1 : Fetal liver lysate
      Lane 2 : PC12 lysate
      Lane 3 : C6 lysate
      Lane 4 : Fetal brain lysate
      Lane 5 : Human spleen lysate
      Lane 6 : Saos 2 lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 11 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9038(B)] (ab154803)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9038(B)] (ab154803)

      Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling UQCRH with ab154803 at 1/50 dilution.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9038(B)] (ab154803)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9038(B)] (ab154803)

      Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling UQCRH with ab154803 at 1/50 dilution.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Flow Cytometry - Anti-UQCRH antibody [EPR9038(B)] (ab154803)
      Flow Cytometry - Anti-UQCRH antibody [EPR9038(B)] (ab154803)
      Flow cytometric analysis of permeabilized Saos 2 cells labeling UQCRH with ab154803 at 1/10 dilution (red) or a rabbit IgG (green- negative control).
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9038(B)] (ab154803)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9038(B)] (ab154803)

      Immunohistochemical analysis of paraffin embedded Human normal brain tissue using ab154803 showing +ve staining.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9038(B)] (ab154803)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9038(B)] (ab154803)

      Immunohistochemical analysis of paraffin embedded Human normal kidney tissue using ab154803 showing +ve staining.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9038(B)] (ab154803)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9038(B)] (ab154803)

      Immunohistochemical analysis of paraffin embedded Human skeletal muscle tissue using ab154803 showing +ve staining.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9038(B)] (ab154803)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9038(B)] (ab154803)

      Immunohistochemical analysis of paraffin embedded Human thyroid gland carcinoma tissue using ab154803 showing +ve staining.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    References

    ab154803 has not yet been referenced specifically in any publications.

    Publishing research using ab154803? Please let us know so that we can cite the reference in this datasheet.

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