RecombinantRabMAb

Recombinant Anti-UQCRH antibody [EPR9038(B)] (ab154803)

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Western blot - Anti-UQCRH antibody [EPR9038(B)] (ab154803)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9038(B)] (ab154803)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9038(B)] (ab154803)
  • Flow Cytometry - Anti-UQCRH antibody [EPR9038(B)] (ab154803)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9038(B)] (ab154803)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9038(B)] (ab154803)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9038(B)] (ab154803)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9038(B)] (ab154803)
  • Anti-UQCRH antibody [EPR9038(B)] (ab154803)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9038(B)] to UQCRH
  • Suitable for: Flow Cyt, WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-UQCRH antibody [EPR9038(B)]
    See all UQCRH primary antibodies

  • Description

    Rabbit monoclonal [EPR9038(B)] to UQCRH

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, WB, IHC-Pmore details
    Unsuitable for: ICC/IF

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human UQCRH. The exact sequence is proprietary.

  • Positive control

    • Fetal liver, PC12, C6, fetal brain, Human spleen and Saos 2 lysates; Human colon and liver tissues; Saos 2 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab154803 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
WB 1/1000 - 1/10000. Predicted molecular weight: 11 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for ICC/IF.

    • Target

    • Relevance

      UQRCH is a component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is part of the mitochondrial respiratory chain. It may play a role in electron transfer between cytochromes c1 and c.

    • Cellular localization

      Mitochondrial. Mitochondrion inner membrane.

    • Database links

      see all

    • Alternative names

      • Complex III subunit 6 antibody
      • Complex III subunit VIII antibody
      • Cytochrome b c1 complex subunit 6 antibody
      • Cytochrome b c1 complex subunit 6 mitochondrial antibody
      • Cytochrome c1 non heme 11 kDa protein antibody
      • High protein , mitochondrial antibody
      • MGC111572 antibody
      • Mitochondrial hinge protein antibody
      • QCR 6 antibody
      • QCR6 antibody
      • Ubiquinol cytochrome c reductase complex 11 kDa protein antibody
      • Ubiquinol cytochrome c reductase complex 11 kDa protein mitochondrial antibody
      • Ubiquinol cytochrome c reductase complex III subunit VIII antibody
      • Ubiquinol cytochrome c reductase hinge protein antibody
      • ubiquinol-cytochrome c reductase hinge protein antibody
      • UQCR8 antibody
      see all

    Images

    • Western blot - Anti-UQCRH antibody [EPR9038(B)] (ab154803)
      Western blot - Anti-UQCRH antibody [EPR9038(B)] (ab154803)
      All lanes : Anti-UQCRH antibody [EPR9038(B)] (ab154803) at 1/1000 dilution

      Lane 1 : Fetal liver lysate
      Lane 2 : PC12 lysate
      Lane 3 : C6 lysate
      Lane 4 : Fetal brain lysate
      Lane 5 : Human spleen lysate
      Lane 6 : Saos 2 lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 11 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9038(B)] (ab154803)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9038(B)] (ab154803)

      Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling UQCRH with ab154803 at 1/50 dilution.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9038(B)] (ab154803)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9038(B)] (ab154803)

      Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling UQCRH with ab154803 at 1/50 dilution.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Flow Cytometry - Anti-UQCRH antibody [EPR9038(B)] (ab154803)
      Flow Cytometry - Anti-UQCRH antibody [EPR9038(B)] (ab154803)
      Flow cytometric analysis of permeabilized Saos 2 cells labeling UQCRH with ab154803 at 1/10 dilution (red) or a rabbit IgG (green- negative control).
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9038(B)] (ab154803)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9038(B)] (ab154803)

      Immunohistochemical analysis of paraffin embedded Human normal brain tissue using ab154803 showing +ve staining.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9038(B)] (ab154803)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9038(B)] (ab154803)

      Immunohistochemical analysis of paraffin embedded Human normal kidney tissue using ab154803 showing +ve staining.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9038(B)] (ab154803)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9038(B)] (ab154803)

      Immunohistochemical analysis of paraffin embedded Human skeletal muscle tissue using ab154803 showing +ve staining.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9038(B)] (ab154803)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9038(B)] (ab154803)

      Immunohistochemical analysis of paraffin embedded Human thyroid gland carcinoma tissue using ab154803 showing +ve staining.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Anti-UQCRH antibody [EPR9038(B)] (ab154803)
      Anti-UQCRH antibody [EPR9038(B)] (ab154803)

    References (1)

    Publishing research using ab154803? Please let us know so that we can cite the reference in this datasheet.

    ab154803 has been referenced in 1 publication.

    • Meng S  et al. The interactome and proteomic responses of ALKBH7 in cell lines by in-depth proteomics analysis. Proteome Sci 17:8 (2019). PubMed: 31889914

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