Recombinant
Anti-UQCRH antibody [EPR9038(B)] (ab154803)
- Datasheet
- References
- Protocols
Overview
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Product nameAnti-UQCRH antibody [EPR9038(B)]
See all UQCRH primary antibodies -
DescriptionRabbit monoclonal [EPR9038(B)] to UQCRH
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Host speciesRabbit
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Tested applicationsSuitable for: IP, Flow Cyt, WB, IHC-Pmore details
Unsuitable for: ICC/IF -
Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide within Human UQCRH. The exact sequence is proprietary.
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Positive control
- Fetal liver, PC12, C6, fetal brain, Human spleen and Saos 2 lysates; Human colon and liver tissues; Saos 2 cells.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at -20ºC.
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Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEPR9038(B)
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IsotypeIgG
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Research areas
Associated products
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab154803 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | 1/10 - 1/100. | |
| Flow Cyt | 1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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| WB | 1/1000 - 1/10000. Predicted molecular weight: 11 kDa. | |
| IHC-P | 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Application notes
Is unsuitable for ICC/IF.
Target
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RelevanceUQRCH is a component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is part of the mitochondrial respiratory chain. It may play a role in electron transfer between cytochromes c1 and c.
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Cellular localizationMitochondrial. Mitochondrion inner membrane.
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Database links
- Entrez Gene: 7388 Human
- Entrez Gene: 66576 Mouse
- Entrez Gene: 366448 Rat
- Omim: 613844 Human
- SwissProt: P07919 Human
- SwissProt: P99028 Mouse
- SwissProt: Q5M9I5 Rat
- Unigene: 481571 Human
see all -
Alternative names
- Complex III subunit 6 antibody
- Complex III subunit VIII antibody
- Cytochrome b c1 complex subunit 6 antibody
see all
Images
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![Western blot - Anti-UQCRH antibody [EPR9038(B)] (ab154803)](https://www.abcam.com/ps/products/154/ab154803/Images/UQCRH-Primary-antibodies-ab154803-1.jpg)
Western blot - Anti-UQCRH antibody [EPR9038(B)] (ab154803)All lanes : Anti-UQCRH antibody [EPR9038(B)] (ab154803) at 1/1000 dilution
Lane 1 : Fetal liver lysate
Lane 2 : PC12 lysate
Lane 3 : C6 lysate
Lane 4 : Fetal brain lysate
Lane 5 : Human spleen lysate
Lane 6 : Saos 2 lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 11 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9038(B)] (ab154803)](https://www.abcam.com/ps/products/154/ab154803/Images/UQCRH-Primary-antibodies-ab154803-2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9038(B)] (ab154803)Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling UQCRH with ab154803 at 1/50 dilution. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9038(B)] (ab154803)](https://www.abcam.com/ps/products/154/ab154803/Images/UQCRH-Primary-antibodies-ab154803-5.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9038(B)] (ab154803)Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling UQCRH with ab154803 at 1/50 dilution. -
![Flow Cytometry - Anti-UQCRH antibody [EPR9038(B)] (ab154803)](https://www.abcam.com/ps/products/154/ab154803/Images/UQCRH-Primary-antibodies-ab154803-4.jpg)
Flow Cytometry - Anti-UQCRH antibody [EPR9038(B)] (ab154803)Flow cytometric analysis of permeabilized Saos 2 cells labeling UQCRH with ab154803 at 1/10 dilution (red) or a rabbit IgG (green- negative control). -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9038(B)] (ab154803)](https://www.abcam.com/ps/products/154/ab154803/Images/UQCRH-Primary-antibodies-ab154803-6.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9038(B)] (ab154803)Immunohistochemical analysis of paraffin embedded Human normal brain tissue using ab154803 showing +ve staining. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9038(B)] (ab154803)](https://www.abcam.com/ps/products/154/ab154803/Images/UQCRH-Primary-antibodies-ab154803-7.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9038(B)] (ab154803)Immunohistochemical analysis of paraffin embedded Human normal kidney tissue using ab154803 showing +ve staining. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9038(B)] (ab154803)](https://www.abcam.com/ps/products/154/ab154803/Images/UQCRH-Primary-antibodies-ab154803-8.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9038(B)] (ab154803)Immunohistochemical analysis of paraffin embedded Human skeletal muscle tissue using ab154803 showing +ve staining. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9038(B)] (ab154803)](https://www.abcam.com/ps/products/154/ab154803/Images/UQCRH-Primary-antibodies-ab154803-9.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9038(B)] (ab154803)Immunohistochemical analysis of paraffin embedded Human thyroid gland carcinoma tissue using ab154803 showing +ve staining.
Datasheets and documents
Protocols
References
ab154803 has not yet been referenced specifically in any publications.
