RecombinantRabMAb

Recombinant Anti-UQCRH antibody [EPR9038(B)] - BSA and Azide free (ab249111)

Submit a review Submit a question
Western blot - Anti-UQCRH antibody [EPR9038(B)] - BSA and Azide free (ab249111)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9038(B)] - BSA and Azide free (ab249111)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9038(B)] - BSA and Azide free (ab249111)
  • Flow Cytometry - Anti-UQCRH antibody [EPR9038(B)] - BSA and Azide free (ab249111)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9038(B)] - BSA and Azide free (ab249111)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9038(B)] - BSA and Azide free (ab249111)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9038(B)] - BSA and Azide free (ab249111)
  • Anti-UQCRH antibody [EPR9038(B)] - BSA and Azide free (ab249111)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9038(B)] to UQCRH - BSA and Azide free
  • Suitable for: IHC-P, Flow Cyt, WB
  • Reacts with: Rat, Human

Overview

  • Product name

    Anti-UQCRH antibody [EPR9038(B)] - BSA and Azide free
    See all UQCRH primary antibodies

  • Description

    Rabbit monoclonal [EPR9038(B)] to UQCRH - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Rat
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab249111 is the carrier-free version of ab154803. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab249111 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab249111 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
IHC-P
Human
WB
Rat
All applications
Mouse
Application Abreviews Notes
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 11 kDa.
Notes
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 11 kDa.
  • Application notes
    Is unsuitable for ICC/IF.

    • Target

    • Relevance

      UQRCH is a component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is part of the mitochondrial respiratory chain. It may play a role in electron transfer between cytochromes c1 and c.

    • Cellular localization

      Mitochondrial. Mitochondrion inner membrane.

    • Database links

      see all

    • Alternative names

      • Complex III subunit 6 antibody
      • Complex III subunit VIII antibody
      • Cytochrome b c1 complex subunit 6 antibody
      • Cytochrome b c1 complex subunit 6 mitochondrial antibody
      • Cytochrome c1 non heme 11 kDa protein antibody
      • High protein , mitochondrial antibody
      • MGC111572 antibody
      • Mitochondrial hinge protein antibody
      • QCR 6 antibody
      • QCR6 antibody
      • Ubiquinol cytochrome c reductase complex 11 kDa protein antibody
      • Ubiquinol cytochrome c reductase complex 11 kDa protein mitochondrial antibody
      • Ubiquinol cytochrome c reductase complex III subunit VIII antibody
      • Ubiquinol cytochrome c reductase hinge protein antibody
      • ubiquinol-cytochrome c reductase hinge protein antibody
      • UQCR8 antibody
      see all

    Images

    • Western blot - Anti-UQCRH antibody [EPR9038(B)] - BSA and Azide free (ab249111)
      Western blot - Anti-UQCRH antibody [EPR9038(B)] - BSA and Azide free (ab249111)
      All lanes : Anti-UQCRH antibody [EPR9038(B)] (ab154803) at 1/1000 dilution

      Lane 1 : Fetal liver lysate
      Lane 2 : PC12 lysate
      Lane 3 : C6 lysate
      Lane 4 : Fetal brain lysate
      Lane 5 : Human spleen lysate
      Lane 6 : Saos 2 lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 11 kDa



      This data was developed using ab154803, the same antibody clone in a different buffer formulation.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9038(B)] - BSA and Azide free (ab249111)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9038(B)] - BSA and Azide free (ab249111)

      This data was developed using ab154803, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling UQCRH with ab154803 at 1/50 dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9038(B)] - BSA and Azide free (ab249111)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9038(B)] - BSA and Azide free (ab249111)

      This data was developed using ab154803, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling UQCRH with ab154803 at 1/50 dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
    • Flow Cytometry - Anti-UQCRH antibody [EPR9038(B)] - BSA and Azide free (ab249111)
      Flow Cytometry - Anti-UQCRH antibody [EPR9038(B)] - BSA and Azide free (ab249111)
      This data was developed using ab154803, the same antibody clone in a different buffer formulation.Flow cytometric analysis of permeabilized Saos 2 cells labeling UQCRH with ab154803 at 1/10 dilution (red) or a rabbit IgG (green- negative control).
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9038(B)] - BSA and Azide free (ab249111)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9038(B)] - BSA and Azide free (ab249111)
      This data was developed using ab154803, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human normal brain tissue using ab154803 showing +ve staining.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9038(B)] - BSA and Azide free (ab249111)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9038(B)] - BSA and Azide free (ab249111)
      This data was developed using ab154803, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human normal kidney tissue using ab154803 showing +ve staining.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9038(B)] - BSA and Azide free (ab249111)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9038(B)] - BSA and Azide free (ab249111)
      This data was developed using ab154803, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human skeletal muscle tissue using ab154803 showing +ve staining.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
    • Anti-UQCRH antibody [EPR9038(B)] - BSA and Azide free (ab249111)
      Anti-UQCRH antibody [EPR9038(B)] - BSA and Azide free (ab249111)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab249111? Please let us know so that we can cite the reference in this datasheet.

    ab249111 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab249111.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com