Recombinant Anti-UQCRH antibody [EPR9038(B)] - BSA and Azide free (ab249111)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9038(B)] to UQCRH - BSA and Azide free
- Suitable for: IHC-P, Flow Cyt, WB
- Reacts with: Rat, Human
Overview
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Product name
Anti-UQCRH antibody [EPR9038(B)] - BSA and Azide free
See all UQCRH primary antibodies -
Description
Rabbit monoclonal [EPR9038(B)] to UQCRH - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
Application Species Flow Cyt HumanIHC-P HumanWB Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab249111 is the carrier-free version of ab154803. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab249111 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR9038(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab249111 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
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Flow Cyt |
Human
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IHC-P |
Human
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WB |
Rat
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All applications |
Mouse
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Application | Abreviews | Notes |
---|---|---|
IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
|
Flow Cyt |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 11 kDa.
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Notes |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Flow Cyt
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 11 kDa. |
Target
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Relevance
UQRCH is a component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is part of the mitochondrial respiratory chain. It may play a role in electron transfer between cytochromes c1 and c. -
Cellular localization
Mitochondrial. Mitochondrion inner membrane. -
Database links
- Entrez Gene: 7388 Human
- Entrez Gene: 66576 Mouse
- Entrez Gene: 366448 Rat
- Omim: 613844 Human
- SwissProt: P07919 Human
- SwissProt: P99028 Mouse
- SwissProt: Q5M9I5 Rat
- Unigene: 481571 Human
see all -
Alternative names
- Complex III subunit 6 antibody
- Complex III subunit VIII antibody
- Cytochrome b c1 complex subunit 6 antibody
see all
Images
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All lanes : Anti-UQCRH antibody [EPR9038(B)] (ab154803) at 1/1000 dilution
Lane 1 : Fetal liver lysate
Lane 2 : PC12 lysate
Lane 3 : C6 lysate
Lane 4 : Fetal brain lysate
Lane 5 : Human spleen lysate
Lane 6 : Saos 2 lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 11 kDaThis data was developed using ab154803, the same antibody clone in a different buffer formulation.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab249111 has not yet been referenced specifically in any publications.