RecombinantRabMAb

Recombinant Anti-UQCRH antibody [EPR9039(B)] (ab134949)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9039(B)] (ab134949)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9039(B)] (ab134949)
  • Western blot - Anti-UQCRH antibody [EPR9039(B)] (ab134949)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9039(B)] (ab134949)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9039(B)] (ab134949)
  • Anti-UQCRH antibody [EPR9039(B)] (ab134949)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9039(B)] to UQCRH
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-UQCRH antibody [EPR9039(B)]
    See all UQCRH primary antibodies

  • Description

    Rabbit monoclonal [EPR9039(B)] to UQCRH

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC/IF

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide within Human UQCRH aa 50-150. The exact sequence is proprietary.

  • Positive control

    • Human colon and thyroid carcinoma tissues; HL60 cells; HL60, fetal muscle, fetal liver, fetal brain, fetal heart, fetal kidney, and Human spleen lysates.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab134949 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 11 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 11 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt or ICC/IF.

    • Target

    • Relevance

      UQRCH is a component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is part of the mitochondrial respiratory chain. It may play a role in electron transfer between cytochromes c1 and c.

    • Cellular localization

      Mitochondrial. Mitochondrion inner membrane.

    • Database links

      see all

    • Alternative names

      • Complex III subunit 6 antibody
      • Complex III subunit VIII antibody
      • Cytochrome b c1 complex subunit 6 antibody
      • Cytochrome b c1 complex subunit 6 mitochondrial antibody
      • Cytochrome c1 non heme 11 kDa protein antibody
      • High protein , mitochondrial antibody
      • MGC111572 antibody
      • Mitochondrial hinge protein antibody
      • QCR 6 antibody
      • QCR6 antibody
      • Ubiquinol cytochrome c reductase complex 11 kDa protein antibody
      • Ubiquinol cytochrome c reductase complex 11 kDa protein mitochondrial antibody
      • Ubiquinol cytochrome c reductase complex III subunit VIII antibody
      • Ubiquinol cytochrome c reductase hinge protein antibody
      • ubiquinol-cytochrome c reductase hinge protein antibody
      • UQCR8 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9039(B)] (ab134949)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9039(B)] (ab134949)

      Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling UQCRH with ab134949 at 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9039(B)] (ab134949)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9039(B)] (ab134949)

      Immunohistochemical analysis of paraffin-embeddedHuman thyroid carcinoma tissue labelling UQCRH with ab134949 at 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Western blot - Anti-UQCRH antibody [EPR9039(B)] (ab134949)
      Western blot - Anti-UQCRH antibody [EPR9039(B)] (ab134949)
      All lanes : Anti-UQCRH antibody [EPR9039(B)] (ab134949) at 1/1000 dilution

      Lane 1 : Fetal muscle lysate
      Lane 2 : Fetal liver lysate
      Lane 3 : HL60 cell lysate
      Lane 4 : Fetal brain lysate
      Lane 5 : Fetal heart lysate
      Lane 6 : Fetal kidney lysate
      Lane 7 : Human spleen lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 11 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9039(B)] (ab134949)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9039(B)] (ab134949)

      Immunohistochemical analysis using ab134949 showing positive staining in Normal kidney tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9039(B)] (ab134949)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRH antibody [EPR9039(B)] (ab134949)

      Immunohistochemical analysis using ab134949 showing positive staining in Normal uterus tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Anti-UQCRH antibody [EPR9039(B)] (ab134949)
      Anti-UQCRH antibody [EPR9039(B)] (ab134949)

    References (4)

    Publishing research using ab134949? Please let us know so that we can cite the reference in this datasheet.

    ab134949 has been referenced in 4 publications.

    • Luo Y  et al. UQCRH downregulation promotes Warburg effect in renal cell carcinoma cells. Sci Rep 10:15021 (2020). PubMed: 32929120
    • Zhang S  et al. Mitochondrial peptide BRAWNIN is essential for vertebrate respiratory complex III assembly. Nat Commun 11:1312 (2020). PubMed: 32161263
    • Song W  et al. Organic cation transporter 3 (Oct3) is a distinct catecholamines clearance route in adipocytes mediating the beiging of white adipose tissue. PLoS Biol 17:e2006571 (2019). PubMed: 30653498
    • Park ER  et al. The mitochondrial hinge protein, UQCRH, is a novel prognostic factor for hepatocellular carcinoma. Cancer Med 6:749-760 (2017). WB . PubMed: 28332314

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