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    uqcrq-antibody-ab241983.pdf

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Signal Transduction Metabolism Mitochondrial
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Anti-UQCRQ antibody (ab241983)

  • Datasheet
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Western blot - Anti-UQCRQ antibody (ab241983)
  • Immunoprecipitation - Anti-UQCRQ antibody (ab241983)

Key features and details

  • Rabbit polyclonal to UQCRQ
  • Suitable for: WB, IP
  • Reacts with: Mouse, Human
  • Isotype: IgG

You may also be interested in

Protein
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Recombinant Human UQCRQ protein (denatured) (ab171691)
Secondary
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-UQCRQ antibody
    See all UQCRQ primary antibodies
  • Description

    Rabbit polyclonal to UQCRQ
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment within Human UQCRQ aa 32-82. The exact sequence is proprietary.
    Database link: O14949

  • Positive control

    • WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysates. IP: HEK-293T whole cell lysate.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 6.8
    Preservative: 0.09% Sodium azide
    Constituents: Tris buffered saline, 0.1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Antibody was affinity purified using an epitope specific to UQCRQ immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Cell Biology
    • Other Antibodies
    • Oxidative Stress
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Oxidative stress
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Antioxidants
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Oxidative phosphorylation
    • Complex III

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
    • Jurkat whole cell lysate (ab30128)
    • NIH 3T3 whole cell lysate (ab7179)
    • Jurkat whole cell lysate (ab7899)
  • Recombinant Protein

    • Recombinant Human UQCRQ protein (denatured) (ab171691)
  • Related Products

    • Recombinant Human UQCRQ protein (denatured) (ab171691)

Applications

Our Abpromise guarantee covers the use of ab241983 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 10 kDa.
IP Use at an assay dependent concentration.

20 μl/mg lysate

 

Target

  • Function

    This is a component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is part of the mitochondrial respiratory chain. This subunit, together with cytochrome b, binds to ubiquinone.
  • Involvement in disease

    Defects in UQCRQ are a cause of mitochondrial complex III deficiency (MT-C3D) [MIM:124000]. A disorder of the mitochondrial respiratory chain resulting in a highly variable phenotype depending on which tissues are affected. Clinical features include mitochondrial encephalopathy, psychomotor retardation, ataxia, severe failure to thrive, liver dysfunction, renal tubulopathy, muscle weakness and exercise intolerance.
  • Sequence similarities

    Belongs to the UQCRQ/QCR8 family.
  • Cellular localization

    Mitochondrion inner membrane.
  • Target information above from: UniProt accession O14949 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 27089 Human
    • Entrez Gene: 22272 Mouse
    • Omim: 612080 Human
    • SwissProt: O14949 Human
    • SwissProt: Q9CQ69 Mouse
    • Unigene: 146602 Human
    • Alternative names

      • Complex III subunit 8 antibody
      • Complex III subunit VIII antibody
      • Cytochrome b-c1 complex subunit 8 antibody
      • QCR8 antibody
      • QCR8_HUMAN antibody
      • QP-C antibody
      • QPC antibody
      • Ubiquinol-cytochrome c reductase complex 9.5 kDa protein antibody
      • Ubiquinol-cytochrome c reductase complex ubiquinone-binding protein QP-C antibody
      • Uqcrq antibody
      see all

    Images

    • Western blot - Anti-UQCRQ antibody (ab241983)
      Western blot - Anti-UQCRQ antibody (ab241983)
      All lanes : Anti-UQCRQ antibody (ab241983) at 1/1000 dilution

      Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
      Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
      Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
      Lane 4 : TCMK-1 (Mouse kidney epithelial cell line) whole cell lysate
      Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate

      Lysates/proteins at 50 µg per lane.

      Developed using the ECL technique.

      Predicted band size: 10 kDa


      Exposure time: 10 seconds


      Lysates prepared using NETN lysis buffer.

    • Immunoprecipitation - Anti-UQCRQ antibody (ab241983)
      Immunoprecipitation - Anti-UQCRQ antibody (ab241983)

      UQCRQ was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) using ab241983 at 20 µL per reaction. Western blot was performed from the immunoprecipitate using ab241983 at 1/100 dilution.

      Lane 1: ab241983 IP in HEK-293T whole cell lysate.
      Lane 2: Control IgG IP in HEK-293T whole cell lysate.

      Detection: Chemiluminescence with an exposure time of 10 seconds

      Lysates prepared in NETN buffer.

       

       

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab241983? Please let us know so that we can cite the reference in this datasheet.

    ab241983 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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