Product nameAnti-UQCRQ antibody
See all UQCRQ primary antibodies
DescriptionRabbit polyclonal to UQCRQ
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Recombinant fragment within Human UQCRQ aa 32-82. The exact sequence is proprietary.
Database link: O14949
- WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysates. IP: HEK-293T whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntibody was affinity purified using an epitope specific to UQCRQ immobilized on solid support.
Our Abpromise guarantee covers the use of ab241983 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 10 kDa.|
|IP||Use at an assay dependent concentration.
20 μl/mg lysate
FunctionThis is a component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is part of the mitochondrial respiratory chain. This subunit, together with cytochrome b, binds to ubiquinone.
Involvement in diseaseDefects in UQCRQ are a cause of mitochondrial complex III deficiency (MT-C3D) [MIM:124000]. A disorder of the mitochondrial respiratory chain resulting in a highly variable phenotype depending on which tissues are affected. Clinical features include mitochondrial encephalopathy, psychomotor retardation, ataxia, severe failure to thrive, liver dysfunction, renal tubulopathy, muscle weakness and exercise intolerance.
Sequence similaritiesBelongs to the UQCRQ/QCR8 family.
Cellular localizationMitochondrion inner membrane.
- Information by UniProt
- Complex III subunit 8 antibody
- Complex III subunit VIII antibody
- Cytochrome b-c1 complex subunit 8 antibody
All lanes : Anti-UQCRQ antibody (ab241983) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (Mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 10 kDa
Exposure time: 10 seconds
Lysates prepared using NETN lysis buffer.
UQCRQ was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) using ab241983 at 20 µL per reaction. Western blot was performed from the immunoprecipitate using ab241983 at 1/100 dilution.
Lane 1: ab241983 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with an exposure time of 10 seconds
Lysates prepared in NETN buffer.
ab241983 has not yet been referenced specifically in any publications.