Recombinant Anti-URB2 antibody [EPR13404-35] (ab181177)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13404-35] to URB2
- Suitable for: WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-URB2 antibody [EPR13404-35]
See all URB2 primary antibodies -
Description
Rabbit monoclonal [EPR13404-35] to URB2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- Jurkat, HeLa, Raji and 293 cell lysates; 293 cells.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
-
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR13404-35 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab181177 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/2000. Detects a band of approximately 171 kDa (predicted molecular weight: 171 kDa).
|
|
ICC/IF |
1/50 - 1/100.
|
Notes |
---|
WB
1/1000 - 1/2000. Detects a band of approximately 171 kDa (predicted molecular weight: 171 kDa). |
ICC/IF
1/50 - 1/100. |
Target
-
Cellular localization
Nucleus > nucleolus. - Information by UniProt
-
Database links
- Entrez Gene: 9816 Human
- SwissProt: Q14146 Human
- Unigene: 533628 Human
-
Alternative names
- C77575 antibody
- KIAA0133 antibody
- mKIAA0133 antibody
see all
Images
-
All lanes : Anti-URB2 antibody [EPR13404-35] (ab181177) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Raji cell lysate
Lane 4 : 293 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 171 kDa
Observed band size: 171 kDa -
Immunofluorescent analysis of 293 cells (paraformaldehyde-fixed, 4%) labeling URB2 with ab181177 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
Certificate of Compliance
References (0)
ab181177 has not yet been referenced specifically in any publications.