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    urea-assay-kit-ab83362.pdf

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Urea Assay Kit (ab83362)

  • Datasheet
  • SDS
  • Protocol Booklet
Reviews (3)Q&A (22)References (40)

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Functional Studies - Urea Assay Kit (ab83362)
  • Functional Studies - Urea Assay Kit (ab83362)
  • Functional Studies - Urea Assay Kit (ab83362)
  • Functional Studies - Urea Assay Kit (ab83362)
  • Functional Studies - Urea Assay Kit (ab83362)
  • Functional Studies - Urea Assay Kit (ab83362)

Key features and details

  • Assay type: Quantitative
  • Detection method: Colorimetric
  • Platform: Microplate reader
  • Assay time: 1 hr
  • Sample type: Cell culture supernatant, Cell Lysate, Plasma, Serum, Tissue Extracts, Urine
  • Sensitivity: 0.5 nmol/well

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Overview

  • Product name

    Urea Assay Kit
    See all Urea kits
  • Detection method

    Colorimetric
  • Sample type

    Cell culture supernatant, Urine, Serum, Plasma, Tissue Extracts, Cell Lysate
  • Assay type

    Quantitative
  • Sensitivity

    > 0.5 nmol/well
  • Range

    0.5 nmol/well - 5 nmol/well
  • Assay time

    1h 00m
  • Product overview

    Urea Assay Kit ab83362 is a rapid, simple, sensitive, and reliable assay used to quantify urea in a variety of samples such as serum, plasma, and urine, etc. It can be used as a blood urea nitrogen assay kit (BUN assay kit).


    In the urea assay protocol, urea is acted on by enzymes to form a product that reacts with a probe to generate color (ODmax=570nm). The absorbance is directly proportional to the concentration of urea in the solution. The kit can detect as low as 0.5 nmol per well or 10 µM of urea.


    Urea assay protocol summary:
    - add samples and standards to wells
    - add reaction mix and incubate for 60 min at 37ºC
    - analyze with microplate reader

  • Notes

    This product is manufactured by BioVision, an Abcam company and was previously called K375 Urea Colorimetric Assay Kit. K375-100 is the same size as the 100 test size of ab83362.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 100 tests 2000 tests
    Converting Enzyme (Lyophilised) Blue 1 vial 20 vials
    Developer (Lyophilised) Orange 1 vial 20 vials
    Enzyme Mix (lyophilized) Green 1 vial 20 vials
    OxiRed Probe in DMSO Red 1 x 200µl 20 x 200µl
    Urea Assay Buffer WM 1 x 25ml 20 x 25ml
    Urea Standard (100mM) Yellow 1 x 100µl 20 x 100µl
  • Research areas

    • Kits/ Lysates/ Other
    • Kits
    • Cell Metabolism Kits
    • Amino Acid Assays
    • Kits/ Lysates/ Other
    • Kits
    • Cell Metabolism Kits
    • Other Metabolism Assay
  • Relevance

    Urea is a waste product which is produced in the liver, dissolved in blood (in a concentration of 2.5 - 7.5 mM), and secreted by the kidneys. Urea also plays a very important role in protein catabolism, removal of toxic ammonia from the body, and the countercurrent system which allows for reabsorption of water and critical ions in the nephrons.
  • Alternative names

    • Carbamide
    • Carbonyl diamide
    • Carbonyldiamine

Images

  • Functional Studies - Urea Assay Kit (ab83362)
    Functional Studies - Urea Assay Kit (ab83362)Howie, R Nicole et al., PloS one?vol. 10,7 e0132520., Fig 6, doi:10.1371/journal.pone.0132520

    ab83362 was used as a BUN assay (Blood urea nitrogen assay) to measure urea levels in 80 female Sprague-Dawley rats (age 10-12 months-old).

  • Functional Studies - Urea Assay Kit (ab83362)
    Functional Studies - Urea Assay Kit (ab83362)

    Urea measured in cell culture supernatants and control medium (MEM), background signal subtracted (duplicates +/- SD).

    The samples were tested neat; 1:4; 1:16 and 1:64. Only the latter two gave reliable results.

  • Functional Studies - Urea Assay Kit (ab83362)
    Functional Studies - Urea Assay Kit (ab83362)

    Urea measured in mouse tissue lysates (mg of extracted protein), background signal subtracted (duplicates +/- SD).

  • Functional Studies - Urea Assay Kit (ab83362)
    Functional Studies - Urea Assay Kit (ab83362)

    Urea measured in cell lysates, background signal subtracted (duplicates; +/- SD).

  • Functional Studies - Urea Assay Kit (ab83362)
    Functional Studies - Urea Assay Kit (ab83362)

    Urea measured in biological fluids, background signal subtracted (duplicates; +/- SD).

  • Functional Studies - Urea Assay Kit (ab83362)
    Functional Studies - Urea Assay Kit (ab83362)
    Example of Urea standard curve using ab83362

Protocols

  • Protocol Booklet

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (40)

Publishing research using ab83362? Please let us know so that we can cite the reference in this datasheet.

ab83362 has been referenced in 40 publications.

  • Sassi L  et al. A Perfusion Bioreactor for Longitudinal Monitoring of Bioengineered Liver Constructs. Nanomaterials (Basel) 11:N/A (2021). PubMed: 33494337
  • Atallah M  et al. Effect of edaravone on pregnant mice and their developing fetuses subjected to placental ischemia. Reprod Biol Endocrinol 19:19 (2021). PubMed: 33549111
  • Yang WY  et al. Injection of hybrid 3D spheroids composed of podocytes, mesenchymal stem cells, and vascular endothelial cells into the renal cortex improves kidney function and replenishes glomerular podocytes. Bioeng Transl Med 6:e10212 (2021). PubMed: 34027096
  • Jones BE  et al. The neutralizing antibody, LY-CoV555, protects against SARS-CoV-2 infection in nonhuman primates. Sci Transl Med 13:N/A (2021). PubMed: 33820835
  • Zabaleta N  et al. An AAV-based, room-temperature-stable, single-dose COVID-19 vaccine provides durable immunogenicity and protection in non-human primates. Cell Host Microbe 29:1437-1453.e8 (2021). PubMed: 34428428
View all Publications for this product

Customer reviews and Q&As

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Submit a review Submit a question

1-10 of 25 Abreviews or Q&A

Urea Assay

Good Good 4/5 (Ease of Use)
Abreviews
Abreviews
abreview image
The kit is very sensitive for measuring cellular as well as secreted urea content from the cell lysate as well as conditioned media
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Aug 08 2022

Works good

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Abreviews
Easy to follow instruction and good assay results..
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Oct 01 2018

Urea Assay Kit

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Abreviews
perfect regarding the researcher and technician
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jan 24 2018

Question


I want to detect urea that has been reacted with amino acids through both of its amine groups. Will this assay work?

Read More

Abcam community

Verified customer

Asked on Feb 03 2014

Answer

We have not tested urea that has been reacted at both amine groups. However, it will likely not work because the enzyme reaction is very specific for urea.

Read More

Abcam Scientific Support

Answered on Feb 03 2014

Question

Will phenol red from my culture media interfere with the OD measurements?

Read More

Abcam community

Verified customer

Asked on Dec 19 2013

Answer

The phenol red could interfere if the amount of medium used is enough to contribute red color to the sample. Typically when diluted medium is used and the volume is made up to 50ul with buffer, the color does not interfere in the assay. Since the probe emits at 570nm and this is the pink-orange-red zone of the spectrum,red color from the medium can interfere if undiluted medium is used.

Read More

Jared Kaminski

Abcam Scientific Support

Answered on Dec 19 2013

Question

What volume of sample should be added to each well?
What is the total volume in each well after the sample and Reaction Mix has been added?

Read More

Abcam community

Verified customer

Asked on Nov 08 2013

Answer





For all samples, including tissue or cell extracts, add 1-25ul sample to each well and volume up to 50ul with Assay Buffer.

Add 50 ul Urea Reaction Mix to each sample or standard.

Final volume in each well will be 100ul.


Read More

Jeremy Kasanov

Abcam Scientific Support

Answered on Nov 08 2013

Question

Our micro plate reader has 450nm & 620nm, will either of these work for this Elisa? Is everything included in this price or do we need to purchase reagents & diluents separately?

Read More

Abcam community

Verified customer

Asked on May 01 2013

Answer


To answer your questions:

1) The desired wavelength for reading the samples is 570 nm.

If the detection band width on the plate reader is ± 30, then a wavelength of ± 30 of 570 can be used, which is 540-600 nm. Otherwise the efficiency of detection of this assay will decrease.
However, from an earlier customer inquiry, the lab replied that they would not recommend reading this assay at 590 nm.

This would then also apply to reading the assay at 450 or 620 nm. This would not be recommended as you might not get a signal at these wavelengths.


2) The kit datasheet described what the kit contains (https://www.abcam.com/ab83362.html)

The protocol lists the additional materials that is required as, but not provided:
Microcentrifuge, Pipettes and pipette tips, Fluorescent or colorimetric microplate reader,  96 well plate, Orbital shaker

Read More

Abcam Scientific Support

Answered on May 01 2013

Question

Product code: 83362
Lot number: GR62096-1
Inquiry: Dear Sir, Greetings from Jinju, South Korea. I have a question with respect to Urea assay - Urea standard curve preparation. 100mM of Urea stock is supplied with the kit. However, if I dilute this stock to 0.5mM and further If I use this as per recommendation I am afraid, am I getting 1, 2, 3, 4, 5 nanomol/well? I think I am getting about 0, 10, 20, 30, 40, 50 micro mol/well !!! Thanks for the help Kind Regards, Raj

Read More

Abcam community

Verified customer

Asked on Sep 21 2012

Answer

Thank you for contacting us.

The calculation is right. We suggest in the protocol diluting the standard to get 0.5milli molar per 1000ul.

This 0.5 is a molar solution means, it is 0.5 milli mol per litre per 1000ul i.e. 0.5 nano mol per micro litre.

so 2ul of this solution would be 1nmol/well

4ul would be 2nmol/well and so on.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Abcam Scientific Support

Answered on Sep 21 2012

Question

One of our customers is interested in ab83362.
The customer wishes to perform a test for urea quantity in common lab buffers such as TRIS and PBS as part of an experiment in which at some point urea is taken out of the buffer.

Do you think the kit is suitable to test for urea quantity and presence in these and similar buffers?

As always, thank you for your kind help and a good week to you.
Regards,

Read More

Abcam community

Verified customer

Asked on Sep 05 2012

Answer

Thank you for contacting us.

I have double checked your question with the laboratory, and I am happy to confirm, that this kit should work with the buffers your client mentions. It will work with all common lab buffers. The only ones it would not work with are the ones which can inactivate the enzymes in this assay kit. Since we cannot disclose all the components in such mixtures, your client will need to clarify it with you/us before changing the buffers.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Abcam Scientific Support

Answered on Sep 05 2012

Question

Dear Abcam,

I am interested in your product urea assay kit (ab83362), but I have a few technical questions.


Could I read the assay at 540 rather than 570?

Does hemolysis affect the reading?

What volumes would be needed of mouse serum samples?
Thanks

Read More

Abcam community

Verified customer

Asked on Aug 13 2012

Answer

Thank you for your enquiry regarding ab83362.
If the detection band width on the plate reader is ± 30, then a wavelength of ± 30 of 570 can be used, which is 540-600 nm. Otherwise the efficiency of detection of this assay will decrease.
Unfortunately, we not have data showing that hemolysis would affect the reading, but I would think that a cleaner serum sample would give better results.
There is no exact amount of mouse serum which will be right for this assay. For unknown samples, we suggest testing several doses of your sample to ensure the readings are within the standard curve range. I would suggest making a dose curve initially to determine the optimal starting sample volume.
I hope this will be useful for you.

Read More

Abcam Scientific Support

Answered on Aug 13 2012

1-10 of 25 Abreviews or Q&A

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