Urea Assay Kit (ab83362)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 1 hr
- Sample type: Cell culture supernatant, Cell Lysate, Plasma, Serum, Tissue Extracts, Urine
- Sensitivity: 0.5 nmol/well
Overview
-
Product name
Urea Assay Kit
See all Urea kits -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Urine, Serum, Plasma, Tissue Extracts, Cell Lysate -
Assay type
Quantitative -
Sensitivity
> 0.5 nmol/well -
Range
0.5 nmol/well - 5 nmol/well -
Assay time
1h 00m -
Product overview
Urea Assay Kit ab83362 is a rapid, simple, sensitive, and reliable assay used to quantify urea in a variety of samples such as serum, plasma, and urine, etc. It can be used as a blood urea nitrogen assay kit (BUN assay kit).
In the urea assay protocol, urea is acted on by enzymes to form a product that reacts with a probe to generate color (ODmax=570nm). The absorbance is directly proportional to the concentration of urea in the solution. The kit can detect as low as 0.5 nmol per well or 10 µM of urea.
Urea assay protocol summary:
- add samples and standards to wells
- add reaction mix and incubate for 60 min at 37ºC
- analyze with microplate reader -
Notes
This product is manufactured by BioVision, an Abcam company and was previously called K375 Urea Colorimetric Assay Kit. K375-100 is the same size as the 100 test size of ab83362.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate reader
Properties
-
Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests 2000 tests Assay Buffer XIV 1 x 25ml 20 x 25ml Converter Enzyme V 1 vial 20 vials Developer I 1 vial 20 vials Development Enzyme Mix I 1 vial 20 vials OxiRed Probe 1 x 200µl 20 x 200µl Urea Standard 1 x 100µl 20 x 100µl -
Research areas
-
Relevance
Urea is a waste product which is produced in the liver, dissolved in blood (in a concentration of 2.5 - 7.5 mM), and secreted by the kidneys. Urea also plays a very important role in protein catabolism, removal of toxic ammonia from the body, and the countercurrent system which allows for reabsorption of water and critical ions in the nephrons. -
Alternative names
- Carbamide
- Carbonyl diamide
- Carbonyldiamine
Images
-
ab83362 was used as a BUN assay (Blood urea nitrogen assay) to measure urea levels in 80 female Sprague-Dawley rats (age 10-12 months-old).
-
Urea measured in cell culture supernatants and control medium (MEM), background signal subtracted (duplicates +/- SD).
The samples were tested neat; 1:4; 1:16 and 1:64. Only the latter two gave reliable results.
-
Urea measured in mouse tissue lysates (mg of extracted protein), background signal subtracted (duplicates +/- SD).
-
Urea measured in cell lysates, background signal subtracted (duplicates; +/- SD).
-
Urea measured in biological fluids, background signal subtracted (duplicates; +/- SD).
-
Example of Urea standard curve using ab83362
Datasheets and documents
-
SDS download
-
Datasheet download
References (84)
ab83362 has been referenced in 84 publications.
- Widjaja AA et al. A Neutralizing IL-11 Antibody Improves Renal Function and Increases Lifespan in a Mouse Model of Alport Syndrome. J Am Soc Nephrol 33:718-730 (2022). PubMed: 35140116
- Farooqi HMU et al. Study of the Anticancer Potential of Plant Extracts Using Liver Tumor Microphysiological System. Life (Basel) 12:N/A (2022). PubMed: 35207423
- Tsai SC et al. Anti-Obesity Effect of Nostoc commune Ethanol Extract In Vitro and In Vivo. Nutrients 14:N/A (2022). PubMed: 35267941
- Du Y et al. Intranasal Lentiviral Vector-Mediated Antibody Delivery Confers Reduction of SARS-CoV-2 Infection in Elderly and Immunocompromised Mice. Front Immunol 13:819058 (2022). PubMed: 35529866
- Kühnlenz J et al. A microfluidic thyroid-liver platform to assess chemical safety in humans. ALTEX N/A:N/A (2022). PubMed: 35536601