Overview

  • Product name

    Urea Assay Kit II
    See all Urea kits
  • Detection method

    Colorimetric
  • Sample type

    Cell culture supernatant, Milk, Urine, Serum, Plasma, Tissue Extracts, Cell Lysate
  • Species reactivity

    Reacts with: Other species, Mammals
  • Product overview

    Urea Assay Kit II (ab234052) is a fast, sensitive and easy to use kit that measures urea level in various tissues/cells. It is based on Jung’s method with a modification that delivers more robust and sensitive data. The urea is condensed with o-phthalaldehyde (OPA), followed by a reaction to form a colored product with strong absorbance at 505 nm. It can measure less than 10 μM urea in 96-well plate assay.


    This urea assay kit provides an alternative to the most popular urea assay kit ab83362, and uses a different assay method.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 100 tests
    Urea Reagent A 1 x 12ml
    Urea Reagent B 1 x 12ml
    Urea Standard (100 mM) 1 x 100µl
  • Research areas

  • Relevance

    Urea is a waste product which is produced in the liver, dissolved in blood (in a concentration of 2.5 - 7.5 mM), and secreted by the kidneys. Urea also plays a very important role in protein catabolism, removal of toxic ammonia from the body, and the countercurrent system which allows for reabsorption of water and critical ions in the nephrons.
  • Alternative names

    • Carbamide
    • Carbonyl diamide
    • Carbonyldiamine

Images

Protocols

References

ab234052 has not yet been referenced specifically in any publications.

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