Urease Activity Assay Kit (Colorimetric) (ab204697)
Key features and details
- Assay type: Enzyme activity
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 1 hr 10 min
- Sample type: Adherent cells, Purified protein, Suspension cells, Urine
- Sensitivity: 0.001 mU/well
Overview
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Product name
Urease Activity Assay Kit (Colorimetric) -
Detection method
Colorimetric -
Sample type
Urine, Adherent cells, Suspension cells, Purified protein -
Assay type
Enzyme activity -
Sensitivity
< 0.001 mU/well -
Assay time
1h 10m -
Product overview
Urease Activity Assay Kit (Colorimetric) (ab204697) provides a quick and easy way to determine urease activity. The kit uses a modified Berthelot method to detect ammonia, which can be measured at 670 nm. The kit is suitable for measuring urease activity of biological and soil samples as well as of purified enzyme. The limit of detection is below 0.001 mU.
Urease assay protocol summary:
- add samples and standards to wells
- add reaction mix and incubate for 30 min
- add additional reagents and incubate for 30 min
- analyze with microplate reader -
Notes
This product is manufactured by BioVision, an Abcam company and was previously called K378 Urease Activity Assay Kit (Colorimetric). K378-100 is the same size as the 100 test size of ab204697.
Urease (EC 3.5.1.5) catalyzes the hydrolysis of urea into carbon dioxide and ammonia. Urease is found in certain bacteria, yeast, fungi, and plants. In addition, it is found environmentally in soil samples and fecal matter. Due to ammonia production, urease can play a role in the pathogenesis of certain medical conditions including urinary stones, peptic ulcers, and hepatic encephalopathy. As such, urease can be used as a diagnostic for gastrointestinal and urinary tract pathogens.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests Ammonia Reagent 1 Amber 1 x 8ml Ammonia Reagent 2 Clear 1 x 4ml Ammonium Chloride Standard (100 mM) 1 x 100µl Urea (50x) 1 x 1.5ml Urease 1 vial Urease Assay Buffer 1 x 15ml -
Research areas
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Relevance
The enzyme urease catalyzes the hydrolysis of urea to create carbon dioxide and ammonia. The protein consists of a single kind of polypeptide chain containing 840 amino acid residues. The subunit relative molecular mass calculated from the sequence is 90,770, indicating that urease is composed of six subunits. -
Cellular localization
Cytoplasmic -
Alternative names
- Urea Amidohydrolase
Images
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Urease Activity Assay Kit (Colorimetric) (ab204697)
Typical ammonia Standard calibration curve using Urease Activity Assay Kit (Colorimetric) (ab204697).
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Urease Activity Assay Kit (Colorimetric) (ab204697)Measurement of Urease activity in Staphylococcus aureus and Saccharomyces cerevisiae (yeast) cell extracts.
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Urease Activity Assay Kit (Colorimetric) (ab204697)Measurement of Urease activity in soil.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab204697 has been referenced in 2 publications.
- Lu Z et al. Functional Changes of the Community of Microbes With Ni-Dependent Enzyme Genes Accompany Adaptation of the Ruminal Microbiome to Urea-Supplemented Diets. Front Microbiol 11:596681 (2020). PubMed: 33414773
- Rizvi A et al. Rewiring of Metabolic Network in Mycobacterium tuberculosis During Adaptation to Different Stresses. Front Microbiol 10:2417 (2019). PubMed: 31736886