For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    urease-activity-assay-kit-colorimetric-ab204697.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Cell Biology Other Antibodies Other Antibodies
Share by email

Urease Activity Assay Kit (Colorimetric) (ab204697)

  • Datasheet
  • SDS
  • Protocol Booklet
Submit a review Submit a question References (2)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Urease Activity Assay Kit (Colorimetric) (ab204697)
  • Urease Activity Assay Kit (Colorimetric) (ab204697)
  • Urease Activity Assay Kit (Colorimetric) (ab204697)

Key features and details

  • Assay type: Enzyme activity
  • Detection method: Colorimetric
  • Platform: Microplate reader
  • Assay time: 1 hr 10 min
  • Sample type: Adherent cells, Purified protein, Suspension cells, Urine
  • Sensitivity: 0.001 mU/well

You may also be interested in

Biochemical
Product image
Cholesterol, water-permeable lipid (ab142501)
ELISA
Product image
Rat CKM ELISA Kit (ab187396)
Assay
Product image
Urea Assay Kit (ab83362)

View more associated products

Overview

  • Product name

    Urease Activity Assay Kit (Colorimetric)
  • Detection method

    Colorimetric
  • Sample type

    Urine, Adherent cells, Suspension cells, Purified protein
  • Assay type

    Enzyme activity
  • Sensitivity

    < 0.001 mU/well
  • Assay time

    1h 10m
  • Product overview

    Urease Activity Assay Kit (Colorimetric) (ab204697) provides a quick and easy way to determine urease activity. The kit uses a modified Berthelot method to detect ammonia, which can be measured at 670 nm. The kit is suitable for measuring urease activity of biological and soil samples as well as of purified enzyme. The limit of detection is below 0.001 mU.


    Urease assay protocol summary:
    - add samples and standards to wells
    - add reaction mix and incubate for 30 min
    - add additional reagents and incubate for 30 min
    - analyze with microplate reader

  • Notes

    This product is manufactured by BioVision, an Abcam company and was previously called K378 Urease Activity Assay Kit (Colorimetric). K378-100 is the same size as the 100 test size of ab204697.

    Urease (EC 3.5.1.5) catalyzes the hydrolysis of urea into carbon dioxide and ammonia. Urease is found in certain bacteria, yeast, fungi, and plants. In addition, it is found environmentally in soil samples and fecal matter. Due to ammonia production, urease can play a role in the pathogenesis of certain medical conditions including urinary stones, peptic ulcers, and hepatic encephalopathy. As such, urease can be used as a diagnostic for gastrointestinal and urinary tract pathogens.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 100 tests
    Ammonia Reagent 1 Amber 1 x 8ml
    Ammonia Reagent 2 Clear 1 x 4ml
    Ammonium Chloride Standard (100 mM) 1 x 100µl
    Urea (50x) 1 x 1.5ml
    Urease 1 vial
    Urease Assay Buffer 1 x 15ml
  • Research areas

    • Cell Biology
    • Other Antibodies
    • Other Antibodies
  • Relevance

    The enzyme urease catalyzes the hydrolysis of urea to create carbon dioxide and ammonia. The protein consists of a single kind of polypeptide chain containing 840 amino acid residues. The subunit relative molecular mass calculated from the sequence is 90,770, indicating that urease is composed of six subunits.
  • Cellular localization

    Cytoplasmic
  • Alternative names

    • Urea Amidohydrolase

Images

  • Urease Activity Assay Kit (Colorimetric) (ab204697)
    Urease Activity Assay Kit (Colorimetric) (ab204697)

    Typical ammonia Standard calibration curve using Urease Activity Assay Kit (Colorimetric) (ab204697).

  • Urease Activity Assay Kit (Colorimetric) (ab204697)
    Urease Activity Assay Kit (Colorimetric) (ab204697)

    Measurement of Urease activity in Staphylococcus aureus and Saccharomyces cerevisiae (yeast) cell extracts.

  • Urease Activity Assay Kit (Colorimetric) (ab204697)
    Urease Activity Assay Kit (Colorimetric) (ab204697)

    Measurement of Urease activity in soil.

Protocols

  • Protocol Booklet

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (2)

Publishing research using ab204697? Please let us know so that we can cite the reference in this datasheet.

ab204697 has been referenced in 2 publications.

  • Lu Z  et al. Functional Changes of the Community of Microbes With Ni-Dependent Enzyme Genes Accompany Adaptation of the Ruminal Microbiome to Urea-Supplemented Diets. Front Microbiol 11:596681 (2020). PubMed: 33414773
  • Rizvi A  et al. Rewiring of Metabolic Network in Mycobacterium tuberculosis During Adaptation to Different Stresses. Front Microbiol 10:2417 (2019). PubMed: 31736886

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

There are currently no Customer reviews or Questions for ab204697.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Get resources and offers direct to your inbox Sign up
A-Z by research area
  • Cancer
  • Cardiovascular
  • Cell biology
  • Developmental biology
  • Epigenetics & Nuclear signaling
  • Immunology
  • Metabolism
  • Microbiology
  • Neuroscience
  • Signal transduction
  • Stem cells
A-Z by product type
  • Primary antibodies
  • Secondary antibodies
  • Biochemicals
  • Isotype controls
  • Flow cytometry multi-color selector
  • Kits
  • Loading controls
  • Lysates
  • Peptides
  • Proteins
  • Slides
  • Tags and cell markers
  • Tools & Reagents
Help & support
  • Support
  • Make an Inquiry
  • Protocols & troubleshooting
  • Placing an order
  • RabMAb products
  • Biochemical product FAQs
  • Training
  • Browse by Target
Company
  • Corporate site
  • Investor relations
  • Company news
  • Careers
  • About us
  • Blog
Events
  • Tradeshows
  • Conferences
International websites
  • abcam.cn
  • abcam.co.jp

Join with us

  • LinkedIn
  • facebook
  • Twitter
  • YouTube
  • Terms of sale
  • Website terms of use
  • Cookie policy
  • Privacy policy
  • Legal
  • Modern slavery statement
© 1998-2022 Abcam plc. All rights reserved.