Overview

  • Product name

    Urease Activity Assay Kit (Colorimetric)
  • Detection method

    Colorimetric
  • Sample type

    Urine, Adherent cells, Suspension cells, Purified protein
  • Assay type

    Enzyme activity
  • Sensitivity

    < 0.001 mU/well
  • Assay time

    1h 10m
  • Product overview

    Urease Activity Assay Kit (Colorimetric) (ab204697) provides a quick and easy way to determine urease activity. The kit uses a modified Berthelot method to detect ammonia, which can be measured at 670 nm. The kit is suitable for measuring urease activity of biological and soil samples as well as of purified enzyme. The limit of detection is below 0.001 mU.


    Urease assay protocol summary:
    - add samples and standards to wells
    - add reaction mix and incubate for 30 min
    - add additional reagents and incubate for 30 min
    - analyze with microplate reader

  • Notes

    Urease (EC 3.5.1.5) catalyzes the hydrolysis of urea into carbon dioxide and ammonia. Urease is found in certain bacteria, yeast, fungi, and plants. In addition, it is found environmentally in soil samples and fecal matter. Due to ammonia production, urease can play a role in the pathogenesis of certain medical conditions including urinary stones, peptic ulcers, and hepatic encephalopathy. As such, urease can be used as a diagnostic for gastrointestinal and urinary tract pathogens.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 100 tests
    Ammonia Reagent 1 Amber 1 x 8ml
    Ammonia Reagent 2 Clear 1 x 4ml
    Ammonium Chloride Standard (100 mM) 1 x 100µl
    Urea (50x) 1 x 1.5ml
    Urease 1 vial
    Urease Assay Buffer 1 x 15ml
  • Research areas

  • Relevance

    The enzyme urease catalyzes the hydrolysis of urea to create carbon dioxide and ammonia. The protein consists of a single kind of polypeptide chain containing 840 amino acid residues. The subunit relative molecular mass calculated from the sequence is 90,770, indicating that urease is composed of six subunits.
  • Cellular localization

    Cytoplasmic
  • Alternative names

    • Urea Amidohydrolase

Images

  • Typical ammonia Standard calibration curve using Urease Activity Assay Kit (Colorimetric) (ab204697).

  • Measurement of Urease activity in Staphylococcus aureus and Saccharomyces cerevisiae (yeast) cell extracts.

  • Measurement of Urease activity in soil.

Protocols

References

ab204697 has not yet been referenced specifically in any publications.

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