Overview

  • Product name

  • Description

    Rabbit polyclonal to ureB
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Recombinant fragment corresponding to ureB aa 341-398.

  • Positive control

    • ureB whole cell lysate; Mice Gastric pylori.
  • General notes

    Keep as concentrated solution.

     This product was previously labelled as Helicobacter pylori urease B, Helicobacter pylori urease

     

Properties

Applications

Our Abpromise guarantee covers the use of ab127916 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50000. Predicted molecular weight: 62 kDa.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. citrate buffer-10mM

Target

  • Function

    Ammonia produced by ureolysis increases the gastric pH thereby providing an environment permissive for colonization of the stomach.
  • Pathway

    Nitrogen metabolism; urea degradation; CO(2) and NH(3) from urea (urease route): step 1/1.
  • Sequence similarities

    Belongs to the urease family.
    Contains 1 urease domain.
  • Post-translational
    modifications

    Carbamylation allows a single lysine to coordinate two nickel ions.
  • Cellular localization

    Cytoplasm. Also associates with the outer membrane upon autolysis of neighboring bacteria.
  • Information by UniProt
  • Alternative names

    • HP_0072 antibody
    • hpuB antibody
    • URE1_HELPY antibody
    • Urea amidohydrolase subunit beta antibody
    • Urease subunit beta antibody
    • ureB antibody
    see all

Images

  • All lanes : Anti-ureB antibody (ab127916) at 1/50000 dilution

    Lane 1 : ureB at 0.5 µg
    Lane 2 : ureB at 1 µg

    Developed using the ECL technique.

    Predicted band size: 62 kDa



    7.5% SDS PAGE
  • ab127916, at 1/500 dilution, staining ureB in paraffin-embedded Mice Gastric pylori, by Immunohistochemistry.

References

This product has been referenced in:

  • Ohishi T  et al. Monotherapy with a novel intervenolin derivative, AS-1934, is an effective treatment for Helicobacter pylori infection. Helicobacter 23:e12470 (2018). WB . Read more (PubMed: 29488678) »
  • Li N  et al. Helicobacter pylori CagA promotes epithelial mesenchymal transition in gastric carcinogenesis via triggering oncogenic YAP pathway. J Exp Clin Cancer Res 37:280 (2018). Read more (PubMed: 30466467) »
See all 2 Publications for this product

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