Product nameAnti-Uric acid antibody
DescriptionRabbit polyclonal to Uric acid
Tested applicationsSuitable for: WB, ELISA, ICC/IFmore details
Synthetic Uric acid conjugated to BSA (Bovine Serum Albumin).
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferConstituent: Whole serum
Concentration information loading...
PurityAmmonium Sulphate Precipitation
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Our Abpromise guarantee covers the use of ab53000 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/2000.|
|ELISA||1/5000 - 1/10000.|
|ICC/IF||1/1000 - 1/5000.|
RelevanceUric acid is a major endogenous antioxidant and peroxynitrite scavenger. Xanthine oxidase oxidizes oxypurines such as xanthine and hypoxanthine to uric acid. In humans and higher primates, uric acid is the final oxidation product of purine catabolism. In most other mammals, the enzyme uricase further oxidizes uric acid to allantoin. The loss of uricase in higher primates parallels the similar loss of the ability to synthesize ascorbic acid. This may be because in higher primates uric acid (urate) partially replaces ascorbic acid. Both urate and ascorbate are strong reducing substances (electron donors) and potent antioxidants. In humans, about half the antioxidant capacity of plasma comes from uric acid.
Lane 2 : Anti-Uric acid antibody (ab53000) at 1/1000 dilution
Lane 1 : Marker
Lane 2 : Human LNCaP whole cell lysate at 60 µg
Lane 2 : IRDye 800CW conjugated goat anti-rabbit IgG polyclonal at 1/15000 dilution
Performed under reducing conditions.
Exposure time: 4 minutes
This product has been referenced in:
- Gold MJ et al. Mucosal production of uric acid by airway epithelial cells contributes to particulate matter-induced allergic sensitization. Mucosal Immunol 9:809-20 (2016). Read more (PubMed: 26509876) »
- van de Hoef DL et al. Plasmodium falciparum-derived uric acid precipitates induce maturation of dendritic cells. PLoS One 8:e55584 (2013). ICC/IF . Read more (PubMed: 23405174) »