Overview

  • Product name
    Anti-Uric acid antibody
  • Description
    Rabbit polyclonal to Uric acid
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ELISA, ICC/IFmore details
  • Immunogen

    Synthetic Uric acid conjugated to BSA (Bovine Serum Albumin).

Properties

Applications

Our Abpromise guarantee covers the use of ab53000 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000.
ELISA 1/5000 - 1/10000.
ICC/IF 1/1000 - 1/5000.

Target

  • Relevance
    Uric acid is a major endogenous antioxidant and peroxynitrite scavenger. Xanthine oxidase oxidizes oxypurines such as xanthine and hypoxanthine to uric acid. In humans and higher primates, uric acid is the final oxidation product of purine catabolism. In most other mammals, the enzyme uricase further oxidizes uric acid to allantoin. The loss of uricase in higher primates parallels the similar loss of the ability to synthesize ascorbic acid. This may be because in higher primates uric acid (urate) partially replaces ascorbic acid. Both urate and ascorbate are strong reducing substances (electron donors) and potent antioxidants. In humans, about half the antioxidant capacity of plasma comes from uric acid.

Images

  • Lane 2 : Anti-Uric acid antibody (ab53000) at 1/1000 dilution

    Lane 1 : Marker
    Lane 2 : Human LNCaP whole cell lysate at 60 µg

    Secondary
    Lane 2 : IRDye 800CW conjugated goat anti-rabbit IgG polyclonal at 1/15000 dilution

    Performed under reducing conditions.

    Exposure time: 4 minutes

    See Abreview

References

This product has been referenced in:
  • Gold MJ  et al. Mucosal production of uric acid by airway epithelial cells contributes to particulate matter-induced allergic sensitization. Mucosal Immunol 9:809-20 (2016). Read more (PubMed: 26509876) »
  • van de Hoef DL  et al. Plasmodium falciparum-derived uric acid precipitates induce maturation of dendritic cells. PLoS One 8:e55584 (2013). ICC/IF . Read more (PubMed: 23405174) »
See all 3 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Application
Western blot
Loading amount
60 µg
Gel Running Conditions
Reduced Non-Denaturing (Native) (12)
Sample
Human Cell lysate - whole cell (LNCaP cell line)
Specification
LNCaP cell line
Blocking step
Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 26°C

Dr. Francesco Elia Marino

Verified customer

Submitted May 15 2014

Answer

Thank you very much for contacting us with your enquiry.
To our knowledge, ab53000 has not been tested in IHC-P. I can offer a free primary antibody on a future purchase if you'd like to purchase ab53000 now, test it in IHC-P, and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback.
If you are interested in this offer, please follow these steps:
1. Reply to this e-mail to let me know that you would like to proceed. I will then send a discount code.
2. Purchase ab53000 either by phone, fax, or online (www.abcam.com).
3. Test it in IHC-P.
4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.
5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue. Please remember that submission of the Abreview is sufficient for the discount code to become active.
We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab53000 turns out to be unsuitable for IHC-P, you will still receive the discount on your next purchase after your Abreview has been submitted.
Please let me know if you have any questions about this offer and I would be happy to help you further.
The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

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Answer

Thank you for contacting Abcam regarding ab53000.


We have not specifically tested this antibody on tissue sections, so we do not have any information about how to use the antibody in this context.


In terms of a positive control, we have specifically tested against the molecule itself in ELISA, but I have attached the following reference in which the antibody was used in neuron cultures.


I hope this information is helpful. Please do not hesitate to contact me if you have any additional questions.

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Question
Answer

DISCOUNT CODE: *
Expiration date: *

I am very pleased to hear you would like to accept our offer and test ab53000 in IHC-P. This code will give you: 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview forIHC-P and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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