Overview

  • Product name

    Uric Acid Assay Kit
  • Detection method

    Colorimetric/Fluorometric
  • Sample type

    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
  • Assay type

    Quantitative
  • Assay time

    0h 40m
  • Product overview

    Uric Acid Assay Kit (ab65344) provides a convenient means for detecting uric acid in biological samples such as serum and urine. Pretreatment of samples is not required.


    Uric acid level can be measured using fluorometric (at Ex/Em = 535/587 nm) or colorimetric (at λ= 570 nm) methods.


    Uric acid assay protocol summary:
    - add reaction mix to samples and standards
    - incubate for 30 min
    - analyze on microplate reader

  • Notes

    Uric acid in serum is the end product of purine metabolism, and is cleared through the kidney by glomerular filtration. However, human often lacks the necessary enzyme called urate oxidase (Uricase), and therefore abnormal uric acid may be accumulated in blood. Recent evidences show the close association between serum urate level and cardiovascular morbidity and mortality, especially among persons at high cardiovascular risk, including those with hypertension, diabetes and congestion heart failure.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 100 tests
    Uric Acid Assay Buffer WM 1 x 25ml
    Uric Acid Enzyme Mix Green 1 vial
    Uric Acid Probe (in DMSO, anhydrous) Red 1 x 0.2ml
    Uric Acid Standard (2 nmol/µl) Yellow 1 x 1ml
  • Research areas

  • Relevance

    Uric acid is a heterocyclic purine derivate that is the final oxidation product of purine metabolism. It is produced by xantine oxidase, which oxidizes oxypurines such as xanthine into uric acid. In most mammals, except humans and higher primates, the enzyme uricase further oxidizes uric acid to allantoin. In humans, however, uric acid is the end product of purine metabolism, and is cleared through the kidney by glomerular filtration and excreted in urine. Humans procude only small quantities of uric acid and therefore abnormal uric acid may be accumulated in blood leading to a type of arthritis known as gout.
  • Alternative names

    • Urate

Images

  • Uric acid measured in mouse tissue lysates, showing quantity (nmol) per mg of extracted protein (duplicates; +/- SD).

  • Uric acid measured in biological fluid (duplicates; +/- SD).

  • Uric acid fluorimetric standard curve.

  • Uric acid colorimetric standard curve.

Protocols

References

This product has been referenced in:

  • Lee SH  et al. Dual actions on gout flare and acute kidney injury along with enhanced renal transporter activities by Yokuininto, a Kampo medicine. BMC Complement Altern Med 19:57 (2019). Read more (PubMed: 30871515) »
  • Wozniak P  et al. The lipid peroxidation in patients with nephrolithiasis before and after extracorporeal shock wave lithotripsy. Future Med Chem 10:2685-2693 (2018). Read more (PubMed: 30518231) »
See all 7 Publications for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Answer

My calculations show that the assay will not be sensitive enough to detect your samples, assuming 0.1 mg/dL.

The range of the standard curve is given as nmol per well, 8 to 40 nmol for the colorimetric assay and 0.8 nmol to 4 for the fluorometric assay. This requires a little arithmetic, since the units are amount (nanomoles) per well, rather than concentration. Here is what I did:

Using the MW of uric acid, one mole is 168g, so 1 nmol is 168 ng.

Assume 0.8 nmol per well is taken as the limit of detection. 0.8 x 168 ng = 134 ng

Assume the concentration of uric acid in rhesus samples is 0.1mg/dL, which is equivalent to 1ng/ul. The volume of the experimental sample is 50 ul, so you are trying to detect 50ng per well, which is below the amount in the lowest standard of the fluorometric assay, 134 ng.

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Answer


Yesterday we talked about ab65344, specifically the additional equipment/materials that you will need to use the kit to measure uric acid in your mouse serum samples. Below I've included a list of the additional materials required with links to potential options for purchasing them. Additionally, the 100 tests that the 1 kit is capable of refers to 100 wells in a 96 well plate, not 100 96 well plates.
From the protocol:

Additional Materials Required

 Microcentrifuge
http://www.thermoscientific.com/ecomm/servlet/productscatalog_11152__81866_-1_4

 Pipettes and pipette tips
I'm assuming you already have these

 Fluorescent or colorimetric microplate reader
http://www.thermoscientific.com/ecomm/servlet/productscatalog?storeId=11152&categoryId=82178&ca=platereaders

 96 well plate (type depends on whether you want to perform fluorescence or colorimetric detection)
Fluorescence: Black plates (clear bottoms)
Colorimetry: Clear plates
http://www.bdbiosciences.com/nvCategory.jsp?action=SELECT&form=formTree_catBean&item=776813

 Orbital shaker (for step 2(part 4) of the protocol, 37oC incubation)
http://www.coleparmer.com/Category/Orbital_Shakers/5366

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Answer

Thank you for your enquiry regarding ab65344.

This is to let you know that I have placed a new order for you - for one kit of ab65344 as a free of charge replacement and the new order number is 1170301.

We will dispatch this item next Monday (together with ab136595 - Nucleosome antigen) and will be shipped on dry ice.

I hope this new kit will work as it is expected, and please do let me know how you are getting on with this product.

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Answer

Thank you for contacting us.

This kits hasn't been tested in samples particularly with saliva however as this kit predicted to be used with biological samples so the kit would work.

Please familiarize the customer with 100% Abreview discount codes. If the customers is interested in testing this kit, a 100% Abreview discount code can be provided.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
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Answer

Thank you for your enquiry. I have contacted the originator of the product, and they have confirmed that this product uses Uricase. If there is anything else that I can help you with, please do not hesitate to contact me.

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Answer

Thank you for your enquiry. I can confirm that in the [Instructions for use/protocol] section of the datasheet, it states that these products (ab65329, ab65344, ab65656) can be used with serum samples. Although product ab65354 does not have the same information, it is recommended for use in detecting SOD activity in tissue homogenates, cell lysates, and other biological fluids/extracts where forms of SOD might be present. I hope this information will be helpful. If you should require any other information, please do not hesitate to contact me.

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