Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric/Fluorometric
- Platform: Microplate reader
- Assay time: 40 min
- Sample type: Cell culture supernatant, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
Product nameUric Acid Assay Kit
Sample typeCell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
Assay time0h 40m
Uric Acid Assay Kit (ab65344) provides a convenient means for detecting uric acid in biological samples such as serum and urine. Pretreatment of samples is not required.
Uric acid level can be measured using fluorometric (at Ex/Em = 535/587 nm) or colorimetric (at λ= 570 nm) methods.
Uric acid assay protocol summary:
- add reaction mix to samples and standards
- incubate for 30 min
- analyze on microplate reader
Uric acid in serum is the end product of purine metabolism, and is cleared through the kidney by glomerular filtration. However, human often lacks the necessary enzyme called urate oxidase (Uricase), and therefore abnormal uric acid may be accumulated in blood. Recent evidences show the close association between serum urate level and cardiovascular morbidity and mortality, especially among persons at high cardiovascular risk, including those with hypertension, diabetes and congestion heart failure.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 100 tests Uric Acid Assay Buffer WM 1 x 25ml Uric Acid Enzyme Mix Green 1 vial Uric Acid Probe (in DMSO, anhydrous) Red 1 x 0.2ml Uric Acid Standard (2 nmol/µl) Yellow 1 x 1ml
RelevanceUric acid is a heterocyclic purine derivate that is the final oxidation product of purine metabolism. It is produced by xantine oxidase, which oxidizes oxypurines such as xanthine into uric acid. In most mammals, except humans and higher primates, the enzyme uricase further oxidizes uric acid to allantoin. In humans, however, uric acid is the end product of purine metabolism, and is cleared through the kidney by glomerular filtration and excreted in urine. Humans procude only small quantities of uric acid and therefore abnormal uric acid may be accumulated in blood leading to a type of arthritis known as gout.
ab65344 has been referenced in 10 publications.
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- Lee SH et al. Dual actions on gout flare and acute kidney injury along with enhanced renal transporter activities by Yokuininto, a Kampo medicine. BMC Complement Altern Med 19:57 (2019). PubMed: 30871515
- Zhang B et al. Urate transport capacity of glucose transporter 9 and urate transporter 1 in cartilage chondrocytes. Mol Med Rep 20:1645-1654 (2019). PubMed: 31257523
- Wozniak P et al. The lipid peroxidation in patients with nephrolithiasis before and after extracorporeal shock wave lithotripsy. Future Med Chem 10:2685-2693 (2018). PubMed: 30518231
- Yoon IS et al. In Vitro and In Vivo Studies on Quercus acuta Thunb. (Fagaceae) Extract: Active Constituents, Serum Uric Acid Suppression, and Xanthine Oxidase Inhibitory Activity. Evid Based Complement Alternat Med 2017:4097195 (2017). PubMed: 28421120
- Gold MJ et al. Mucosal production of uric acid by airway epithelial cells contributes to particulate matter-induced allergic sensitization. Mucosal Immunol 9:809-20 (2016). PubMed: 26509876
- Petrasek J et al. Metabolic danger signals, uric acid and ATP, mediate inflammatory cross-talk between hepatocytes and immune cells in alcoholic liver disease. J Leukoc Biol 98:249-56 (2015). PubMed: 25934928
- Girard S et al. Circulating cytokines and alarmins associated with placental inflammation in high-risk pregnancies. Am J Reprod Immunol 72:422-34 (2014). PubMed: 24867252
- Maslanik T et al. The inflammasome and danger associated molecular patterns (DAMPs) are implicated in cytokine and chemokine responses following stressor exposure. Brain Behav Immun N/A:N/A (2012). PubMed: 23103443