• Product name
    Uric Acid Assay Kit
  • Sample type
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
  • Assay type
  • Assay time
    0h 40m
  • Product overview

    Uric Acid Assay Kit (ab65344) provides a convenient means for detecting uric acid in biological samples such as serum and urine. Pretreatment of samples are not required. Uric acid level can be measured using fluorometric (at Ex/Em = 535/587 nm) or colorimetric (at λ= 570 nm) methods.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Uric acid in serum is the end product of purine metabolism, and is cleared through the kidney by glomerular filtration. However, human often lacks the necessary enzyme called urate oxidase (Uricase), and therefore abnormal uric acid may be accumulated in blood. Recent evidences show the close association between serum urate level and cardiovascular morbidity and mortality, especially among persons at high cardiovascular risk, including those with hypertension, diabetes and congestion heart failure.


  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components Identifier 100 tests
    Uric Acid Assay Buffer WM 1 x 25ml
    Uric Acid Enzyme Mix Green 1 vial
    Uric Acid Probe (in DMSO, anhydrous) Red 1 x 0.2ml
    Uric Acid Standard (2 nmol/µl) Yellow 1 x 1ml
  • Research areas
  • Relevance
    Uric acid is a heterocyclic purine derivate that is the final oxidation product of purine metabolism. It is produced by xantine oxidase, which oxidizes oxypurines such as xanthine into uric acid. In most mammals, except humans and higher primates, the enzyme uricase further oxidizes uric acid to allantoin. In humans, however, uric acid is the end product of purine metabolism, and is cleared through the kidney by glomerular filtration and excreted in urine. Humans procude only small quantities of uric acid and therefore abnormal uric acid may be accumulated in blood leading to a type of arthritis known as gout.
  • Alternative names
    • Urate


  • Uric acid measured in mouse tissue lysates, showing quantity (nmol) per mg of extracted protein (duplicates; +/- SD).

  • Uric acid measured in biological fluid (duplicates; +/- SD).

  • Uric Acid Standard Curve.



This product has been referenced in:
  • Yoon IS  et al. In Vitro and In Vivo Studies on Quercus acuta Thunb. (Fagaceae) Extract: Active Constituents, Serum Uric Acid Suppression, and Xanthine Oxidase Inhibitory Activity. Evid Based Complement Alternat Med 2017:4097195 (2017). Read more (PubMed: 28421120) »
  • Girard S  et al. Circulating cytokines and alarmins associated with placental inflammation in high-risk pregnancies. Am J Reprod Immunol 72:422-34 (2014). Read more (PubMed: 24867252) »

See all 3 Publications for this product

Customer reviews and Q&As

My calculations show that the assay will not be sensitive enough to detect your samples, assuming 0.1 mg/dL.

The range of the standard curve is given as nmol per well, 8 to 40 nmol for the colorimetric assay and 0.8 nmol to 4 for the fluorom...

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Yesterday we talked about ab65344, specifically the additional equipment/materials that you will need to use the kit to measure uric acid in your mouse serum samples. Below I've included a list of the additional materials required with links to po...

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Thank you for your enquiry regarding ab65344.

This is to let you know that I have placed a new order for you - for one kit of ab65344 as a free of charge replacement and the new order number is 1170301.

We will dispatch this item ne...

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Thank you for contacting us.

This kits hasn't been tested in samples particularly with saliva however as this kit predicted to be used with biological samples so the kit would work.

Please familiarize the customer with 100% Abreview d...

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Thank you for your enquiry. I have contacted the originator of the product, and they have confirmed that this product uses Uricase. If there is anything else that I can help you with, please do not hesitate to contact me.

Thank you for your enquiry. I can confirm that in the [Instructions for use/protocol] section of the datasheet, it states that these products (ab65329, ab65344, ab65656) can be used with serum samples. Although product ab65354 does not have the sam...

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