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We are interested in the Uric Acid assay kit that you have. Can this kit be used for Rhesus Monkey; the issue is that Rhesus Monkey have lower concentrations of uric acid than many other species; concentration can be as low as 0.1 mg/dl. Does your kit have the accuracy to measure Uric acid concentrations at that level?
Asked on Mar 12 2013
My calculations show that the assay will not be sensitive enough to detect your samples, assuming 0.1 mg/dL.
The range of the standard curve is given as nmol per well, 8 to 40 nmol for the colorimetric assay and 0.8 nmol to 4 for the fluorometric assay. This requires a little arithmetic, since the units are amount (nanomoles) per well, rather than concentration. Here is what I did:
Using the MW of uric acid, one mole is 168g, so 1 nmol is 168 ng.
Assume 0.8 nmol per well is taken as the limit of detection. 0.8 x 168 ng = 134 ng
Assume the concentration of uric acid in rhesus samples is 0.1mg/dL, which is equivalent to 1ng/ul. The volume of the experimental sample is 50 ul, so you are trying to detect 50ng per well, which is below the amount in the lowest standard of the fluorometric assay, 134 ng.
Answered on Mar 12 2013