Overview

  • Product name

    Uricase Assay Kit (Fluorometric)
  • Detection method

    Fluorescent
  • Sample type

    Tissue Extracts
  • Assay type

    Enzyme activity
  • Sensitivity

    10 µU
  • Assay time

    1h 00m
  • Species reactivity

    Reacts with: Other species, Mammals
  • Product overview

    Uricase Assay Kit (Fluorometric) (ab234042) provides a quick and easy method for the measurement of uricase activity in a wide variety of samples.


    In this assay, uricase oxidizes uric acid forming a product that reacts with the probe, thus generating a fluorescence signal (Ex/Em= 535/587nm). The generated fluorescence signal is directly proportional to the level of uricase activity in the sample. The kit provides a sensitive, high throughput and adaptable assay that can measure uricase activity as low as 10 µU in biological samples.

  • Notes

    Uricase (factor-independent urate hydroxylase, urate oxidase (UO); EC 1.7.3.3) is an enzyme involved in the uric acid metabolism. Uric acid is the end product of purine metabolism, and high levels of uric acid in blood causes gout. Uricase is present in a wide range of mammals but absent from human beings.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 100 tests
    H2O2 Standard (0.88 M) 1 x 100µl
    Uricase Assay Buffer 1 x 50ml
    Uricase Enzyme Mix 1 vial
    Uricase Positive Control 1 vial
    Uricase Probe (in DMSO) 1 x 200µl
    Uricase Substrate 1 x 10ml
  • Research areas

  • Relevance

    Uricase catalyzes the oxidation of uric acid to 5 hydroxyisourate which spontaneously decomposes to form allantoin.
  • Cellular localization

    Peroxisome.
  • Alternative names

    • Factor-independent urate hydroxylase
    • UOX
    • Urate oxidase
    • urate oxidase, pseudogene
    • URICASE
    see all

Images

  • Uricase activity in rat liver (5.57 μg protein), spinach leaves (31.5 μg protein) and Uricase Positive Control.

Protocols

References

ab234042 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab234042.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up