Anti-Uridine Phosphorylase 1 antibody (ab185680)
Key features and details
- Rabbit polyclonal to Uridine Phosphorylase 1
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-Uridine Phosphorylase 1 antibody
See all Uridine Phosphorylase 1 primary antibodies -
Description
Rabbit polyclonal to Uridine Phosphorylase 1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human Uridine Phosphorylase 1 aa 1-100 (N terminal).
Database link: Q16831 -
Positive control
- IHC-P: Human esophagus tissue. ICC/IF: U-2 OS cells.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab185680 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P | (1) |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
|
Notes |
---|
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
Target
-
Function
Catalyzes the reversible phosphorylytic cleavage of uridine and deoxyuridine to uracil and ribose- or deoxyribose-1-phosphate. The produced molecules are then utilized as carbon and energy sources or in the rescue of pyrimidine bases for nucleotide synthesis. -
Pathway
Pyrimidine metabolism; UMP biosynthesis via salvage pathway; uracil from uridine (phosphorylase route): step 1/1. -
Sequence similarities
Belongs to the PNP/UDP phosphorylase family. - Information by UniProt
-
Database links
- Entrez Gene: 7378 Human
- Omim: 191730 Human
- SwissProt: Q16831 Human
- Unigene: 488240 Human
-
Alternative names
- UDRPASE antibody
- UP antibody
- UPase 1 antibody
see all
Images
-
Paraffin embedded human esophagus tissue stained for Uridine Phosphorylase 1 using ab185680 at 1/200 dilution in immunohistochemical analysis.
Heat mediated antigen retrieval was performed with citrate buffer pH 6 before the IHC staining protocol.
-
U-2 OS cells stained for Uridine Phosphorylase 1 (green) using ab185680 at 2 µg/ml in ICC/IF.
-
Paraffin embedded human stomach tissue stained for Uridine Phosphorylase 1 using ab185680 at 1/200 dilution in immunohistochemical analysis. Shows low expression as expected.
Heat mediated antigen retrieval was performed with citrate buffer pH 6 before the IHC staining protocol.
Datasheets and documents
-
SDS download
-
Datasheet download
References (1)
ab185680 has been referenced in 1 publication.
- Wang X et al. UPP1 Promotes Lung Adenocarcinoma Progression through Epigenetic Regulation of Glycolysis. Aging Dis 13:1488-1503 (2022). PubMed: 36186123