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    uridine-phosphorylase-1-antibody-ab185680.pdf

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Anti-Uridine Phosphorylase 1 antibody (ab185680)

  • Datasheet
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uridine Phosphorylase 1 antibody (ab185680)
  • Immunocytochemistry/ Immunofluorescence - Anti-Uridine Phosphorylase 1 antibody (ab185680)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uridine Phosphorylase 1 antibody (ab185680)

Key features and details

  • Rabbit polyclonal to Uridine Phosphorylase 1
  • Suitable for: IHC-P, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

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Protein
Recombinant Human Uridine Phosphorylase 1 protein (ab172847)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-Uridine Phosphorylase 1 antibody
    See all Uridine Phosphorylase 1 primary antibodies
  • Description

    Rabbit polyclonal to Uridine Phosphorylase 1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human Uridine Phosphorylase 1 aa 4-47 (N terminal).
    Sequence:

    TGANAEKAESHNDCPVRLLNPNIAKMKEDILYHFNLTTSRHNFP


    Database link: Q16831
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • IHC-P: Human esophagus tissue. ICC/IF: U-2 OS cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA / Nucleotides
    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Cancer
    • Tumor biomarkers
    • Enzymes
    • Other
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Nucleotide metabolism
    • Purine and pyrimidine synthesis
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human Uridine Phosphorylase 1 protein (ab172847)
  • Related Products

    • Recombinant Human Uridine Phosphorylase 1 protein (ab101152)

Applications

Our Abpromise guarantee covers the use of ab185680 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 0.25 - 2 µg/ml.

Target

  • Function

    Catalyzes the reversible phosphorylytic cleavage of uridine and deoxyuridine to uracil and ribose- or deoxyribose-1-phosphate. The produced molecules are then utilized as carbon and energy sources or in the rescue of pyrimidine bases for nucleotide synthesis.
  • Pathway

    Pyrimidine metabolism; UMP biosynthesis via salvage pathway; uracil from uridine (phosphorylase route): step 1/1.
  • Sequence similarities

    Belongs to the PNP/UDP phosphorylase family.
  • Target information above from: UniProt accession Q16831 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 7378 Human
    • Omim: 191730 Human
    • SwissProt: Q16831 Human
    • Unigene: 488240 Human
    • Alternative names

      • UDRPASE antibody
      • UP antibody
      • UPase 1 antibody
      • UPASE antibody
      • UPP antibody
      • Upp1 antibody
      • UPP1_HUMAN antibody
      • UrdPase 1 antibody
      • Uridine phosphorylase 1 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uridine Phosphorylase 1 antibody (ab185680)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uridine Phosphorylase 1 antibody (ab185680)

      Paraffin embedded human esophagus tissue stained for Uridine Phosphorylase 1 using ab185680 at 1/200 dilution in immunohistochemical analysis.

      Heat mediated antigen retrieval was performed with citrate buffer pH 6 before the IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-Uridine Phosphorylase 1 antibody (ab185680)
      Immunocytochemistry/ Immunofluorescence - Anti-Uridine Phosphorylase 1 antibody (ab185680)

      U-2 OS cells stained for Uridine Phosphorylase 1 (green) using ab185680 at 2 µg/ml in ICC/IF. 

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uridine Phosphorylase 1 antibody (ab185680)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uridine Phosphorylase 1 antibody (ab185680)

      Paraffin embedded human stomach tissue stained for Uridine Phosphorylase 1 using ab185680 at 1/200 dilution in immunohistochemical analysis. Shows low expression as expected.

      Heat mediated antigen retrieval was performed with citrate buffer pH 6 before the IHC staining protocol.

    Protocols

    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab185680? Please let us know so that we can cite the reference in this datasheet.

    ab185680 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
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    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Uridine Phosphorylase 1 antibody

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Baboon Tissue sections (Adult testis)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Sodium citrate
    Permeabilization
    No
    Specification
    Adult testis
    Blocking step
    5% serum + 3% BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 31 2017

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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