Recombinant
RabMAb

Recombinant Anti-Uridine Phosphorylase 1 antibody [EPR7680] (ab128854)

Overview

  • Product name

    Anti-Uridine Phosphorylase 1 antibody [EPR7680]
    See all Uridine Phosphorylase 1 primary antibodies
  • Description

    Rabbit monoclonal [EPR7680] to Uridine Phosphorylase 1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Uridine Phosphorylase 1. The exact sequence is proprietary.

  • Positive control

    • 293T cell lysate; HeLa cell lysate; HCT116 cell lysate; Human fetal kidney tissue lysate
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab128854 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 31 kDa (predicted molecular weight: 34 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • Target

    • Function

      Catalyzes the reversible phosphorylytic cleavage of uridine and deoxyuridine to uracil and ribose- or deoxyribose-1-phosphate. The produced molecules are then utilized as carbon and energy sources or in the rescue of pyrimidine bases for nucleotide synthesis.
    • Pathway

      Pyrimidine metabolism; UMP biosynthesis via salvage pathway; uracil from uridine (phosphorylase route): step 1/1.
    • Sequence similarities

      Belongs to the PNP/UDP phosphorylase family.
    • Information by UniProt
    • Database links

    • Alternative names

      • UDRPASE antibody
      • UP antibody
      • UPase 1 antibody
      • UPASE antibody
      • UPP antibody
      • Upp1 antibody
      • UPP1_HUMAN antibody
      • UrdPase 1 antibody
      • Uridine phosphorylase 1 antibody
      see all

    Images

    • All lanes : Anti-Uridine Phosphorylase 1 antibody [EPR7680] (ab128854) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : HCT116 cell lysate
      Lane 4 : Human fetal kidney tissue lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 34 kDa

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab128854 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Lung cancer)
    Loading amount
    25 µg
    Specification
    Lung cancer
    Treatment
    shUpp1
    Gel Running Conditions
    Reduced Denaturing (12)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

    Abcam user community

    Verified customer

    Submitted May 08 2013

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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