Product nameUrinary Prostacyclin ELISA kit
Intra-assay Sample n Mean SD CV% Low 24 23pg/ml 9.1% Medium 24 66pg/ml 3.4% High 24 200pg/ml 2.9% Inter-assay Sample n Mean SD CV% Low 8 23pg/ml 9.6% Medium 8 72pg/ml 5.4% High 8 223pg/ml 3.4%
Sample typeCell culture supernatant, Urine, Tissue Culture Media
Range7.81 pg/ml - 2000 pg/ml
Sample specific recovery Sample type Average % Range Urine 101.9 % - % Tissue Culture Media 109.2 % - %
Assay time3h 0m
Assay durationMultiple steps standard assay
Species reactivityReacts with: Human
Predicted to work with: a wide range of other species
Abcam’s Human Urinary Prostacyclin in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Prostacyclin breakdown products in urine. The kit measures the two major breakdown products of Prostacyclin in urine, 6-keto-Prostaglandin F1 alpha (6k-PGF1 alpha) and 2,3-dinor-6-keto-Prostaglandin F1 alpha (2,3d-6k-PGF1 alpha).
A donkey anti-sheep IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated- Urinary Prostacyclin antigen and a polyclonal sheep antibody specific to Urinary Prostacyclin. After incubation the excess reagents are washed away. pNpp substrate is added and after a short incubation the enzyme reaction is stopped and the yellow color generated is read at 405 nm. The intensity of the yellow coloration is inversely proportional to the amount of Urinary Prostacyclin captured in the plate.
Prostacyclin (PGI2) is involved in platelet aggregation, vasoconstriction, and reproductive functions. PGI2 has a half life of 60 minutes in plasma but only 2 to 3 minutes in buffer. The production of PGI2 is typically monitored by measurement of 6k-PGF1α and 2,3d-6k- PGF1α. 6-keto- PGF1α is produced by the non-enzymatic hydration of PGI2, and is further metabolized in urine to the 2,3-dinor derivative. A number of pharmaceuticals alter and/or inhibit the synthesis of PGI2. Methods to measure PGI2 in urine typically involve HPLC, gas chromatography/mass spectrometry, or radioimmunoassay and enzyme immunoassay for 6k- PGF1α.
The cross reactivity for a number of related eicosanoid compounds was determined.
Compound Cross Reactivity 2,3-dinor-6-keto-PGF1α 100% 6-keto-PGF1α 89.14% PGF1α 13.21% PGF2α 4.99% 13,14 dihydro-PGF1α 2.55% 15-keto-PGF2α 0.32% PGE1 0.08% PGD2 0.03% 6,15-diketo-13,14 dihydro-PGF1α 0.03% PGB1 0.01% PGE2 0.01% PGA2 0.01% TXB2 0.01%
Tested applicationsSuitable for: Competitive ELISAmore details
Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 27ml Assay Buffer 1 x 27ml Donkey anti-sheep IgG Microplate (12 x 8 wells) 1 unit Human Urinary Prostacyclin Standard 1 x 500µl Plate Sealer 1 unit pNpp Substrate 1 x 20ml Stop Solution 1 x 5ml Urinary Prostacyclin Alkaline Phosphatase Conjugate 1 x 5ml Urinary Prostacyclin Antibody 1 x 5ml
- 2,3-dinor-6-keto-Prostaglandin F1 alpha
- 2,3d-6k- PGF1 alpha
- 6-keto-Prostaglandin F1 alpha
Our Abpromise guarantee covers the use of ab133032 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Competitive ELISA||Use at an assay dependent concentration.|
This product has been referenced in:
- Umeda S et al. Enhanced Pulmonary Vascular and Alveolar Development via Prenatal Administration of a Slow-Release Synthetic Prostacyclin Agonist in Rat Fetal Lung Hypoplasia. PLoS One 11:e0161334 (2016). Read more (PubMed: 27529478) »