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Abcam's Urine 5-methylcytosine Quantification Kit (Colorimetric) is a complete set of optimized buffers and reagents to colorimetrically quantify 5-mC in urine using an inhibitory competitive immunoassay method. It is suitable for detecting total urinary 5-methylcytosine levels, resulting from whole body turnover or degradation of methylated DNA/RNA in urine from humans and animals. The urine samples can be in fresh or frozen form.
Nucleobase 5-methylcytosine (5-mC), a modified form of cytosine converted by cytosine methyltransferases, is widespread both in DNA and different cellular RNAs. The biological importance of DNA 5-mC methylation as a major epigenetic modification in phenotype and gene expression has been widely recognized. Recent data strongly suggests that RNA 5-mC methylation is also involved in the regulation of various biological processes including tRNA stability/recognition and mRNA translation.
Urinary excretion of 5-mC including both 5-methyl-2-deoxycytidine and 5-methylcytidine is an indication of a whole body turnover or degradation of methylated DNA and RNA. The urinary 5-mC level can be altered by a change of the bodies’ turnover of methylated DNA/RNA or alteration of cellular DNA/RNA methylation status. A number of studies have indicated that 5-mC excreted in urine has the potential to act as a cancer biomarker, with an increased level arising from disease onset and progression. For example, an elevated level of urinary 5-mC was observed in lung cancer, breast cancer, and leukemia patients with active disease states. It was also shown that urinary 5-mC excretion is effected by Alzheimer’s disease or by radiation treatment. It is suggested that urinary 5-mC might be applicable as a biological marker for detecting some types of cancer and monitoring cancer progression after treatment with radiation or demethylation reagents.
|Components||48 tests||96 tests|
|1000X Capture Antibody||1 x 4µl||1 x 8µl|
|10X Wash Buffer||1 x 14ml||1 x 28ml|
|2000X Detection Antibody||1 x 4µl||1 x 8µl|
|320X 5-mC DNA Solution||1 x 15µl||1 x 30µl|
|5-mC Standard, 4 µg/mL||1 x 10µl||1 x 20µl|
|Assay Plate||1 x 6 units||1 x 12 units|
|Binding Solution||1 x 5ml||1 x 10ml|
|Developer Solution||1 x 5ml||1 x 10ml|
|Enhancer Solution||1 x 4µl||1 x 8µl|
|Negative Control, 50 µg/mL||1 x 10µl||1 x 20µl|
|Sample Preparation Plate||1 x 6 units||1 x 12 units|
|Stop Solution||1 x 5ml||1 x 10ml|
ab156903 has not yet been referenced specifically in any publications.