Overview

  • Product name
    Urine 5-methylcytosine Quantification Kit (Colorimetric)
    See all 5-methylcytosine kits
  • Sample type
    Urine
  • Assay type
    Quantitative
  • Sensitivity
    0.03 ng/well
  • Range
    0.05 ng - 1 ng
  • Assay time
    4h 0m
  • Species reactivity
    Reacts with: Human, a wide range of other species
  • Product overview

    Abcam's Urine 5-methylcytosine Quantification Kit (Colorimetric) is a complete set of optimized buffers and reagents to colorimetrically quantify 5-mC in urine using an inhibitory competitive immunoassay method. It is suitable for detecting total urinary 5-methylcytosine levels, resulting from whole body turnover or degradation of methylated DNA/RNA in urine from humans and animals. The urine samples can be in fresh or frozen form.

  • Notes

    Nucleobase 5-methylcytosine (5-mC), a modified form of cytosine converted by cytosine methyltransferases, is widespread both in DNA and different cellular RNAs. The biological importance of DNA 5-mC methylation as a major epigenetic modification in phenotype and gene expression has been widely recognized. Recent data strongly suggests that RNA 5-mC methylation is also involved in the regulation of various biological processes including tRNA stability/recognition and mRNA translation.

    Urinary excretion of 5-mC including both 5-methyl-2-deoxycytidine and 5-methylcytidine is an indication of a whole body turnover or degradation of methylated DNA and RNA. The urinary 5-mC level can be altered by a change of the bodies’ turnover of methylated DNA/RNA or alteration of cellular DNA/RNA methylation status. A number of studies have indicated that 5-mC excreted in urine has the potential to act as a cancer biomarker, with an increased level arising from disease onset and progression. For example, an elevated level of urinary 5-mC was observed in lung cancer, breast cancer, and leukemia patients with active disease states. It was also shown that urinary 5-mC excretion is effected by Alzheimer’s disease or by radiation treatment. It is suggested that urinary 5-mC might be applicable as a biological marker for detecting some types of cancer and monitoring cancer progression after treatment with radiation or demethylation reagents.

Properties

  • Storage instructions
    Please refer to protocols.
  • Components 48 tests 96 tests
    1000X Capture Antibody 1 x 4µl 1 x 8µl
    10X Wash Buffer 1 x 14ml 1 x 28ml
    2000X Detection Antibody 1 x 4µl 1 x 8µl
    320X 5-mC DNA Solution 1 x 15µl 1 x 30µl
    5-mC Standard, 4 µg/mL 1 x 10µl 1 x 20µl
    Assay Plate 1 x 6 units 1 x 12 units
    Binding Solution 1 x 5ml 1 x 10ml
    Developer Solution 1 x 5ml 1 x 10ml
    Enhancer Solution 1 x 4µl 1 x 8µl
    Negative Control, 50 µg/mL 1 x 10µl 1 x 20µl
    Sample Preparation Plate 1 x 6 units 1 x 12 units
    Stop Solution 1 x 5ml 1 x 10ml
  • Research areas

Images

  • 5-mC level is quantified from different volumes of human urine using ab156903.
  • 5-mC standard was added into the assay wells at different concentrations and then measured with ab156903.

Protocols

References

ab156903 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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