Product nameUrine 5-methylcytosine Quantification Kit (Colorimetric)
See all 5-methylcytosine kits
Range0.05 ng - 1 ng
Assay time4h 0m
Species reactivityReacts with: Human, a wide range of other species
Abcam's Urine 5-methylcytosine Quantification Kit (Colorimetric) is a complete set of optimized buffers and reagents to colorimetrically quantify 5-mC in urine using an inhibitory competitive immunoassay method. It is suitable for detecting total urinary 5-methylcytosine levels, resulting from whole body turnover or degradation of methylated DNA/RNA in urine from humans and animals. The urine samples can be in fresh or frozen form.
Nucleobase 5-methylcytosine (5-mC), a modified form of cytosine converted by cytosine methyltransferases, is widespread both in DNA and different cellular RNAs. The biological importance of DNA 5-mC methylation as a major epigenetic modification in phenotype and gene expression has been widely recognized. Recent data strongly suggests that RNA 5-mC methylation is also involved in the regulation of various biological processes including tRNA stability/recognition and mRNA translation.
Urinary excretion of 5-mC including both 5-methyl-2-deoxycytidine and 5-methylcytidine is an indication of a whole body turnover or degradation of methylated DNA and RNA. The urinary 5-mC level can be altered by a change of the bodies’ turnover of methylated DNA/RNA or alteration of cellular DNA/RNA methylation status. A number of studies have indicated that 5-mC excreted in urine has the potential to act as a cancer biomarker, with an increased level arising from disease onset and progression. For example, an elevated level of urinary 5-mC was observed in lung cancer, breast cancer, and leukemia patients with active disease states. It was also shown that urinary 5-mC excretion is effected by Alzheimer’s disease or by radiation treatment. It is suggested that urinary 5-mC might be applicable as a biological marker for detecting some types of cancer and monitoring cancer progression after treatment with radiation or demethylation reagents.
Storage instructionsPlease refer to protocols.
Components 48 tests 96 tests 1000X Capture Antibody 1 x 4µl 1 x 8µl 10X Wash Buffer 1 x 14ml 1 x 28ml 2000X Detection Antibody 1 x 4µl 1 x 8µl 320X 5-mC DNA Solution 1 x 15µl 1 x 30µl 5-mC Standard, 4 µg/mL 1 x 10µl 1 x 20µl Assay Plate 1 x 6 units 1 x 12 units Binding Solution 1 x 5ml 1 x 10ml Developer Solution 1 x 5ml 1 x 10ml Enhancer Solution 1 x 4µl 1 x 8µl Negative Control, 50 µg/mL 1 x 10µl 1 x 20µl Sample Preparation Plate 1 x 6 units 1 x 12 units Stop Solution 1 x 5ml 1 x 10ml
- Methylated DNA Quantification Kit (Colorimetric) (ab117128)
- Methylated DNA Quantification Kit (Fluorometric) (ab117129)
- Hydroxymethylated DNA Quantification Kit (Colorimetric) (ab117130)
- Hydroxymethylated DNA Quantification Kit (Fluorometric) (ab117131)
- Hydroxymethylated DNA Immunoprecipitation (hMeDIP) Kit (ab117134)
- Urine 5-methylcytosine Quantification Kit (Fluorometric) (ab156904)
ab156903 has not yet been referenced specifically in any publications.