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    urm1-antibody-ab4755.pdf

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Cell Biology Proteolysis / Ubiquitin Proteasome / Ubiquitin Ub-like Proteins
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Anti-Urm1 antibody (ab4755)

  • Datasheet
  • SDS
Reviews (1)Q&A (4)

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Western blot - Anti-Urm1 antibody (ab4755)

    Key features and details

    • Rabbit polyclonal to Urm1
    • Suitable for: WB
    • Reacts with: Saccharomyces cerevisiae
    • Isotype: IgG

    You may also be interested in

    Protein
    Product image
    Recombinant Human Urm1 protein (ab105598)
    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

    View more associated products

    Overview

    • Product name

      Anti-Urm1 antibody
      See all Urm1 primary antibodies
    • Description

      Rabbit polyclonal to Urm1
    • Host species

      Rabbit
    • Tested Applications & Species

      Application Species
      WB
      Recombinant fragment
      See all applications and species data
    • Immunogen

      Recombinant full length protein (S. cerevisiae).

    • Positive control

      • Most yeast cell lysates can be used as a positive control without induction or stimulation.
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

      One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

      Learn more here.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      Preservative: 0.01% Sodium azide
      Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
    • Concentration information loading...
    • Purity

      IgG fraction
    • Purification notes

      Purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum.
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Cell Biology
      • Proteolysis / Ubiquitin
      • Proteasome / Ubiquitin
      • Ub-like Proteins

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant Human Urm1 protein (ab105598)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab4755 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Recombinant fragment
    Application Abreviews Notes
    WB (1)
    1/1000. Detects a band of approximately 12 kDa.
    Notes
    WB
    1/1000. Detects a band of approximately 12 kDa.

    Target

    • Function

      Acts as a sulfur carrier required for 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Serves as sulfur donor in tRNA 2-thiolation reaction by thiocarboxylated (-COSH) at its C-terminus by MOCS3. The sulfur is then transferred to tRNA to form 2-thiolation of mcm(5)S(2)U. May also act as an ubiquitin-like protein that is covalently conjugated to other proteins; the relevance of such function is however unclear in vivo.
    • Pathway

      tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.
    • Sequence similarities

      Belongs to the URM1 family.
    • Post-translational
      modifications

      C-terminal thiocarboxylation occurs in 2 steps, it is first acyl-adenylated (-COAMP) via the hesA/moeB/thiF part of MOCS3, then thiocarboxylated (-COSH) via the rhodanese domain of MOCS3.
    • Cellular localization

      Cytoplasm.
    • Target information above from: UniProt accession Q9BTM9 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • SwissProt: P40554 Saccharomyces cerevisiae
      • Alternative names

        • 2900073H19Rik antibody
        • C9orf74 antibody
        • Chromosome 9 open reading frame 74 antibody
        • MGC2668 antibody
        • RGD1306599 antibody
        • RP11 339B21.4 antibody
        • RP23-443G7.1 antibody
        • Ubiquitin related modifier 1 antibody
        • Ubiquitin related modifier 1 homolog antibody
        • Ubiquitin-related modifier 1 homolog antibody
        • Urm 1 antibody
        • URM1 antibody
        • URM1_HUMAN antibody
        see all

      Images

      • Western blot - Anti-Urm1 antibody (ab4755)
        Western blot - Anti-Urm1 antibody (ab4755)

        ab4755 was tested by immunoblot against yeast lysates expressing the Saccharomyces cerevisiae Urm1-GFP fusion protein and other UBL fusion proteins. All UBLs possess limited homology to Ubiquitin and to each other, therefore it is important to know the degree of reactivity of each antibody against each UBL.   Panel A shows total protein staining using ponceau.  Panel B shows positions of free GFP or GFP containing recombinant proteins present in each lysate preparation after reaction with a 1/1000 dilution of Goat polyclonal to GFP (ab6673) followed by reaction with a 1/15000 dilution of Donkey polyclonal to Goat IgG (HRP)(ab7125).  Panel C shows specific reaction with Urm1 using a 1/1000 dilution of ab4755 followed by reaction with a 1/15000 dilution of Goat polyclonal to Rabbit IgG (HRP) (ab7090).  All primary antibodies were diluted in TTBS buffer supplemented with 5% non-fat milk and incubated with the membranes overnight at 4° C.  Yeast lysate proteins were separated by

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (0)

      Publishing research using ab4755? Please let us know so that we can cite the reference in this datasheet.

      ab4755 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

      Show All Reviews Q&A
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      1-5 of 5 Abreviews or Q&A

      Western blot abreview for Anti-Urm1 antibody

      Poor
      Abreviews
      Abreviews
      Application
      Western blot
      Sample
      Schizosaccharomyces pombe Cell lysate - whole cell (Yeast cells)
      Loading amount
      50 µg
      Specification
      Yeast cells
      Gel Running Conditions
      Non-reduced Denaturing
      Blocking step
      BSA as blocking agent for 45 minute(s) · Concentration: 10% · Temperature: 25°C
      Read More

      Abcam user community

      Verified customer

      Submitted Dec 13 2011

      Question

      Thank you for your kind offer. We have decided to test ab4755, if that is possible. I f you could send us the discount code, that would be great.

      Read More

      Abcam community

      Verified customer

      Asked on Oct 31 2011

      Answer

      I am very pleased to hear you would like to accept our offer and test ab4755 in S. pombe. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for S. pombe and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways:  1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the  promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

      Read More

      Abcam Scientific Support

      Answered on Oct 31 2011

      Question

      Anti-Urm1 antibody, reacting with S. pombe

      Read More

      Abcam community

      Verified customer

      Asked on Oct 28 2011

      Answer

      Thank you for your enquiry. I was passed your details by Aris in our marketing department. We have two antibodies that may be of interest: ab4755  Anti-Urm1 antibody      ab110699  Anti-Urm1 antibody [EPR4809]   To our knowledge, these antibodies have not been tested in S. pombe.  Therefore, I can offer a discount off a future purchase if you buy one of these products now, test it in S. pombe and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody.   If you are interested in this offer, please follow these steps: 1.   Reply to this e-mail to let me know that you would like to proceed and test one of the products in S. pombe. I will then send a discount code. This code must be issued before purchasing  the product so please wait for my reply before ordering. 2.   Purchase the antibody either by phone, fax, or online (www.abcam.com). 3.   Test it in S. pombe 4.   Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!).  To find out how to submit an Abreview, please visit:  https://www.abcam.com/abreviews. 5.   After the review is submitted to us, the discount code becomes active.  Simply place your new order by phone, fax, or on the web and mention the discount code.   The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue.   We are always pleased to obtain feedback about our products and any information is greatly appreciated!  Even if the product turns out to be unsuitable, you will still receive the discount on your next purchase after your Abreview has been submitted.   Please let me know if you have any questions about this offer and I would be happy to help you further. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

      Read More

      Abcam Scientific Support

      Answered on Oct 28 2011

      Question

      thank you for your swift reply. However, it only answers my question/concern partially. The most interesting use of the antibody is the detection of Urm1 conjugates and not the detection of unconjugated Urm1p (not only for me but also for most other potential customers). Thus, it is quite important to use the antibody following a protocol that would enable the users to detect those conjugates. This is an information that you don't supply and the difference is unlikely to just reflect the exposure time. Could you therefore give me the contact information of your source of the antibody to be able to discuss this issue directly. Since the antibody is not cheap I would like to use it as efficiently and effectively as I can.

      Read More

      Abcam community

      Verified customer

      Asked on May 17 2006

      Answer

      Thank you for getting back in touch with me. I have been in touch with the source of this antibody and they have confirmed that this antibody was indeed NOT applied in the Furukawa K et al. 2000 publication. The source of this antibody commented: "The data was collected with the aide of a collaborator who is an expert in the field of UBLs (not Furakawa). The reagent detects all URM1, that is any free URM1 in cell lysates as well as URM1 conjugated proteins as our data suggests. The Furakawa reference in a general reference and did not specifically use our product". I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

      Read More

      Abcam Scientific Support

      Answered on May 23 2006

      Question

      Furukawa K et al. A protein conjugation system in yeast with homology to biosynthetic enzyme reaction of prokaryotes. J Biol Chem 275:7462-5 (2000). PubMed: 10713047

      Read More

      Abcam community

      Verified customer

      Asked on May 15 2006

      Answer

      Thank you for your enquiry. I have been in touch with the source of this antibody and they found it difficult to comment on the differences between those supplied for us on our datasheet and those documented in Furukawa et al., 1999. This antibody was applied in the aforementioned reference. The exposure timing was significantly more that that indicated in the western blot on the datasheet enabling the detection of Urm conjugates in addition to free Urm. In the western blot on the datasheet the antibody detects Urm1 and conjugated Urm1 in the yeast cell lysates. Again this blot was taken with an exposure that did not enable the detection of Urm conjugates as shown by the multiple bands in the publication. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

      Read More

      Abcam Scientific Support

      Answered on May 15 2006

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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