Overview

  • Product name
  • Description
    Rabbit polyclonal to Urm1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Saccharomyces cerevisiae
  • Immunogen

    Recombinant full length protein (S. cerevisiae).

  • Positive control
    • Most yeast cell lysates can be used as a positive control without induction or stimulation.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
  • Concentration information loading...
  • Purity
    IgG fraction
  • Purification notes
    Purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab4755 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 12 kDa.
ELISA 1/2000 - 1/10000.

Target

  • Function
    Acts as a sulfur carrier required for 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Serves as sulfur donor in tRNA 2-thiolation reaction by thiocarboxylated (-COSH) at its C-terminus by MOCS3. The sulfur is then transferred to tRNA to form 2-thiolation of mcm(5)S(2)U. May also act as an ubiquitin-like protein that is covalently conjugated to other proteins; the relevance of such function is however unclear in vivo.
  • Pathway
    tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.
  • Sequence similarities
    Belongs to the URM1 family.
  • Post-translational
    modifications
    C-terminal thiocarboxylation occurs in 2 steps, it is first acyl-adenylated (-COAMP) via the hesA/moeB/thiF part of MOCS3, then thiocarboxylated (-COSH) via the rhodanese domain of MOCS3.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
    • Alternative names
      • 2900073H19Rik antibody
      • C9orf74 antibody
      • Chromosome 9 open reading frame 74 antibody
      • MGC2668 antibody
      • RGD1306599 antibody
      • RP11 339B21.4 antibody
      • RP23-443G7.1 antibody
      • Ubiquitin related modifier 1 antibody
      • Ubiquitin related modifier 1 homolog antibody
      • Ubiquitin-related modifier 1 homolog antibody
      • Urm 1 antibody
      • URM1 antibody
      • URM1_HUMAN antibody
      see all

    Images

    • ab4755 was tested by immunoblot against yeast lysates expressing the Urm1-GFP fusion protein and other UBL fusion proteins. All UBLs possess limited homology to Ubiquitin and to each other, therefore it is important to know the degree of reactivity of each antibody against each UBL.   Panel A shows total protein staining using ponceau.  Panel B shows positions of free GFP or GFP containing recombinant proteins present in each lysate preparation after reaction with a 1/1000 dilution of Goat polyclonal to GFP (ab6673) followed by reaction with a 1/15000 dilution of Donkey polyclonal to Goat IgG (HRP)(ab7125).  Panel C shows specific reaction with Urm1 using a 1/1000 dilution of ab4755 followed by reaction with a 1/15000 dilution of Goat polyclonal to Rabbit IgG (HRP) (ab7090).  All primary antibodies were diluted in TTBS buffer supplemented with 5% non-fat milk and incubated with the membranes overnight at 4° C.  Yeast lysate proteins were separated by

    References

    ab4755 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    1-5 of 5 Abreviews or Q&A

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Western blot
    Sample
    Schizosaccharomyces pombe Cell lysate - whole cell (Yeast cells)
    Loading amount
    50 µg
    Specification
    Yeast cells
    Gel Running Conditions
    Non-reduced Denaturing
    Blocking step
    BSA as blocking agent for 45 minute(s) · Concentration: 10% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Dec 13 2011

    Answer

    I am very pleased to hear you would like to accept our offer and test ab4755 in S. pombe. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for S. pombe and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways:  1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the  promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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    Question
    Answer

    Thank you for your enquiry. I was passed your details by Aris in our marketing department. We have two antibodies that may be of interest: ab4755  Anti-Urm1 antibody      ab110699  Anti-Urm1 antibody [EPR4809]   To our knowledge, these antibodies have not been tested in S. pombe.  Therefore, I can offer a discount off a future purchase if you buy one of these products now, test it in S. pombe and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody.   If you are interested in this offer, please follow these steps: 1.   Reply to this e-mail to let me know that you would like to proceed and test one of the products in S. pombe. I will then send a discount code. This code must be issued before purchasing  the product so please wait for my reply before ordering. 2.   Purchase the antibody either by phone, fax, or online (www.abcam.com). 3.   Test it in S. pombe 4.   Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!).  To find out how to submit an Abreview, please visit:  https://www.abcam.com/abreviews. 5.   After the review is submitted to us, the discount code becomes active.  Simply place your new order by phone, fax, or on the web and mention the discount code.   The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue.   We are always pleased to obtain feedback about our products and any information is greatly appreciated!  Even if the product turns out to be unsuitable, you will still receive the discount on your next purchase after your Abreview has been submitted.   Please let me know if you have any questions about this offer and I would be happy to help you further. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

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    Answer

    Thank you for getting back in touch with me. I have been in touch with the source of this antibody and they have confirmed that this antibody was indeed NOT applied in the Furukawa K et al. 2000 publication. The source of this antibody commented: "The data was collected with the aide of a collaborator who is an expert in the field of UBLs (not Furakawa). The reagent detects all URM1, that is any free URM1 in cell lysates as well as URM1 conjugated proteins as our data suggests. The Furakawa reference in a general reference and did not specifically use our product". I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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    Answer

    Thank you for your enquiry. I have been in touch with the source of this antibody and they found it difficult to comment on the differences between those supplied for us on our datasheet and those documented in Furukawa et al., 1999. This antibody was applied in the aforementioned reference. The exposure timing was significantly more that that indicated in the western blot on the datasheet enabling the detection of Urm conjugates in addition to free Urm. In the western blot on the datasheet the antibody detects Urm1 and conjugated Urm1 in the yeast cell lysates. Again this blot was taken with an exposure that did not enable the detection of Urm conjugates as shown by the multiple bands in the publication. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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