- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-Urm1 antibody
See all Urm1 primary antibodies -
DescriptionRabbit polyclonal to Urm1
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Host speciesRabbit
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Tested applicationsSuitable for: WB, ELISAmore details
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Species reactivityReacts with: Saccharomyces cerevisiae
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Immunogen
Recombinant full length protein (S. cerevisiae).
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Positive control
- Most yeast cell lysates can be used as a positive control without induction or stimulation.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
PurityIgG fraction
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Purification notesPurified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab4755 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000. Detects a band of approximately 12 kDa. | |
| ELISA | 1/2000 - 1/10000. |
Target
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FunctionActs as a sulfur carrier required for 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Serves as sulfur donor in tRNA 2-thiolation reaction by thiocarboxylated (-COSH) at its C-terminus by MOCS3. The sulfur is then transferred to tRNA to form 2-thiolation of mcm(5)S(2)U. May also act as an ubiquitin-like protein that is covalently conjugated to other proteins; the relevance of such function is however unclear in vivo.
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PathwaytRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.
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Sequence similaritiesBelongs to the URM1 family.
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Post-translational
modificationsC-terminal thiocarboxylation occurs in 2 steps, it is first acyl-adenylated (-COAMP) via the hesA/moeB/thiF part of MOCS3, then thiocarboxylated (-COSH) via the rhodanese domain of MOCS3. -
Cellular localizationCytoplasm.
- Information by UniProt
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Database links
- SwissProt: P40554 Saccharomyces cerevisiae
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Alternative names
- 2900073H19Rik antibody
- C9orf74 antibody
- Chromosome 9 open reading frame 74 antibody
see all
Images
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Western blot - Anti-Urm1 antibody (ab4755)ab4755 was tested by immunoblot against yeast lysates expressing the Urm1-GFP fusion protein and other UBL fusion proteins. All UBLs possess limited homology to Ubiquitin and to each other, therefore it is important to know the degree of reactivity of each antibody against each UBL. Panel A shows total protein staining using ponceau. Panel B shows positions of free GFP or GFP containing recombinant proteins present in each lysate preparation after reaction with a 1/1000 dilution of Goat polyclonal to GFP (ab6673) followed by reaction with a 1/15000 dilution of Donkey polyclonal to Goat IgG (HRP)(ab7125). Panel C shows specific reaction with Urm1 using a 1/1000 dilution of ab4755 followed by reaction with a 1/15000 dilution of Goat polyclonal to Rabbit IgG (HRP) (ab7090). All primary antibodies were diluted in TTBS buffer supplemented with 5% non-fat milk and incubated with the membranes overnight at 4° C. Yeast lysate proteins were separated by
Protocols
References
ab4755 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
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