Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab77433 is purified by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab77433 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 19 kDa (predicted molecular weight: 18 kDa).|
RelevanceUrocortin 3 is a member of the sauvagine/corticotropin-releasing factor/urotensin I family. It is structurally related to the corticotropin-releasing factor (CRF) gene and the encoded product is an endogenous ligand for CRF type 2 receptors. In the brain it may be responsible for the effects of stress on appetite. In spite of the gene family name similarity, the product of this gene has no sequence similarity to urotensin II. A powerful cytoprotective effect of urocortin peptide administration against ischemia and reperfusion injury has been demonstrated in isolated cardiomyocyte models, as well as in the intact heart both in vitro and in vivo.
- MGC119002 antibody
- OTTHUMP00000018999 antibody
- Prepro urocortin 3 antibody
Lanes 1-2 : Anti-Urocortin 3 antibody (ab77433) at 1 µg/ml
Lane 3 : Anti DYKDDDDK-Tag antibody at 1/3000 dilution
Lane 1 : HEK293 cell lysate overexpressing Urocortin 3 with DYKDDDDK tag at 10 µg
Lane 2 : Mock-transfected HEK293 cell
Lane 3 : HEK293 cell lysate overexpressing Urocortin 3 with DYKDDDDK tag at 35 µg
Developed using the ECL technique.
Predicted band size: 18 kDa
Observed band size: 19 kDa why is the actual band size different from the predicted?
ab77433 has not yet been referenced specifically in any publications.