Key features and details
- Goat polyclonal to Urocortin 3
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Urocortin 3 antibody
See all Urocortin 3 primary antibodies
DescriptionGoat polyclonal to Urocortin 3
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab77433 is purified by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab77433 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 19 kDa (predicted molecular weight: 18 kDa).
A 1 hour primary incubation is recommended for this product.
RelevanceUrocortin 3 is a member of the sauvagine/corticotropin-releasing factor/urotensin I family. It is structurally related to the corticotropin-releasing factor (CRF) gene and the encoded product is an endogenous ligand for CRF type 2 receptors. In the brain it may be responsible for the effects of stress on appetite. In spite of the gene family name similarity, the product of this gene has no sequence similarity to urotensin II. A powerful cytoprotective effect of urocortin peptide administration against ischemia and reperfusion injury has been demonstrated in isolated cardiomyocyte models, as well as in the intact heart both in vitro and in vivo.
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Lanes 1-2 : Anti-Urocortin 3 antibody (ab77433) at 1 µg/ml
Lane 3 : Anti DYKDDDDK-Tag antibody at 1/3000 dilution
Lane 1 : HEK293 cell lysate overexpressing Urocortin 3 with DYKDDDDK tag at 10 µg
Lane 2 : Mock-transfected HEK293 cell
Lane 3 : HEK293 cell lysate overexpressing Urocortin 3 with DYKDDDDK tag at 35 µg
Developed using the ECL technique.
Predicted band size: 18 kDa
Observed band size: 19 kDa why is the actual band size different from the predicted?
ab77433 has not yet been referenced specifically in any publications.