Urokinase Activity Assay Kit (Fluorometric) (ab174098)
- Datasheet
- References
- Protocols
Overview
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Product nameUrokinase Activity Assay Kit (Fluorometric)
See all Urokinase type Plasminogen Activator kits -
Detection methodFluorescent
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Sample typeSerum, Cell culture extracts, Nuclear Extracts, Purified protein
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Assay typeEnzyme activity (quantitative)
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Sensitivity>= 0.02 IU/ml
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Species reactivityReacts with: Other species, Mammals
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Product overview
Urokinase Activity Assay Kit (Fluorometric) (ab174098) uses an AMC-based peptide substrate containing the recognition sequence for Urokinase. Urokinase present in the sample catalyzes the cleavage of the substrate and releases AMC, which can be easily quantified by measuring its fluorescence at Ex/Em = 350/450 nm. This sensitive assay detects very low levels of Urokinase activity in biological samples as well as purified enzymes (as low as 0.02 IU/ml).
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Notes
Urokinase type plasminogen activator (uPA) is a highly restricted serine protease that converts the zymogen plasminogen to active plasmin, a broad-spectrum serine proteinase capable of degrading most of the major protein components of the extracellular matrix. Binding of uPA to its receptor and subsequent uPA mediated pericellular proteolysis are involved in many process including cell migration and tissue remodelling in angiogenesis, atherogenesis, tumor cell metastasis, and ovulation. High level of uPA is a poor prognostic marker for aggressive breast cancer, aggressive prostate cancer, bladder cancer and gastric cancer.
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PlatformMicroplate reader
Properties
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Storage instructionsStore at -20°C. Please refer to protocols.
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Components 100 tests Cell Lysis Buffer 1 x 25ml Human Urokinase (Standard/Positive Control) 1 vial Urokinase Assay Buffer 1 x 25ml Urokinase Substrate 1 x 0.2ml -
Research areas
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FunctionSpecifically cleaves the zymogen plasminogen to form the active enzyme plasmin.
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Tissue specificityExpressed in the prostate gland and prostate cancers.
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Involvement in diseaseQuebec platelet disorder
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Sequence similaritiesBelongs to the peptidase S1 family.
Contains 1 EGF-like domain.
Contains 1 kringle domain.
Contains 1 peptidase S1 domain. -
Post-translational
modificationsPhosphorylation of Ser-158 and Ser-323 abolishes proadhesive ability but does not interfere with receptor binding. -
Cellular localizationSecreted.
- Information by UniProt
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Alternative names
- ATF
- BDPLT5
- EC 3.4.21
see all
Images
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Urokinase Activity Standard CurvesThis is example data only.
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Urokinase Activity Standard CurvesThis is example data only.
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Urokinase activities in various samplesUrokinase activities of Jurkat cell extract [10 µl], Hela nuclear extract [10 µl] and Pooled human serum (10 µl). Assays were performed following the kit protocol. This is example data only.
Protocols
References
ab174098 has not yet been referenced specifically in any publications.
