Urokinase Activity Assay Kit (Fluorometric) (ab174098)
Key features and details
- Assay type: Enzyme activity (quantitative)
- Detection method: Fluorescent
- Platform: Microplate reader
- Sample type: Cell culture extracts, Nuclear Extracts, Purified protein, Serum
- Sensitivity: 0.02 IU/ml
Overview
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Product name
Urokinase Activity Assay Kit (Fluorometric)
See all Urokinase kits -
Detection method
Fluorescent -
Sample type
Serum, Cell culture extracts, Nuclear Extracts, Purified protein -
Assay type
Enzyme activity (quantitative) -
Sensitivity
>= 0.02 IU/ml -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
Urokinase Activity Assay Kit (Fluorometric) (ab174098) uses an AMC-based peptide substrate containing the recognition sequence for Urokinase. Urokinase present in the sample catalyzes the cleavage of the substrate and releases AMC, which can be easily quantified by measuring its fluorescence at Ex/Em = 350/450 nm. This sensitive assay detects very low levels of Urokinase activity in biological samples as well as purified enzymes (as low as 0.02 IU/ml).
Visit our FAQs page for tips and troubleshooting.
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Notes
This product is manufactured by BioVision, an Abcam company and was previously called K728 Urokinase Activity Fluorometric Assay Kit. K728-100 is the same size as the 100 test size of ab174098.
Urokinase type plasminogen activator (uPA) is a highly restricted serine protease that converts the zymogen plasminogen to active plasmin, a broad-spectrum serine proteinase capable of degrading most of the major protein components of the extracellular matrix. Binding of uPA to its receptor and subsequent uPA mediated pericellular proteolysis are involved in many process including cell migration and tissue remodelling in angiogenesis, atherogenesis, tumor cell metastasis, and ovulation. High level of uPA is a poor prognostic marker for aggressive breast cancer, aggressive prostate cancer, bladder cancer and gastric cancer.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Cell Lysis Buffer 1 x 25ml Human Urokinase (Standard/Positive Control) 1 vial Urokinase Assay Buffer 1 x 25ml Urokinase Substrate 1 x 0.2ml -
Research areas
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Function
Specifically cleave the zymogen plasminogen to form the active enzyme plasmin. -
Tissue specificity
Expressed in the prostate gland and prostate cancers. -
Sequence similarities
Belongs to the peptidase S1 family.
Contains 1 EGF-like domain.
Contains 1 kringle domain.
Contains 1 peptidase S1 domain. -
Post-translational
modificationsPhosphorylation of Ser-158 and Ser-323 abolishes proadhesive ability but does not interfere with receptor binding. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- ATF
- ATF uPA
- BDPLT5
see all
Images
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Urokinase Activity Standard Curves
This is example data only.
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Urokinase Activity Standard CurvesThis is example data only.
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Urokinase activities in various samplesUrokinase activities of Jurkat cell extract [10 µl], Hela nuclear extract [10 µl] and Pooled human serum (10 µl). Assays were performed following the kit protocol. This is example data only.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab174098 has been referenced in 2 publications.
- Hsieh HY et al. Hyaluronic acid on the urokinase sustained release with a hydrogel system composed of poloxamer 407: HA/P407 hydrogel system for drug delivery. PLoS One 15:e0227784 (2020). PubMed: 32160196
- Álvarez E et al. Protective, repairing and fibrinolytic effects of rivaroxaban on vascular endothelium. Br J Clin Pharmacol 84:280-291 (2018). PubMed: 28940408