Key features and details
- Assay type: Enzyme activity (quantitative)
- Detection method: Fluorescent
- Platform: Microplate reader
- Sample type: Cell culture extracts, Nuclear Extracts, Purified protein, Serum
- Sensitivity: 0.02 IU/ml
Product nameUrokinase Activity Assay Kit (Fluorometric)
See all Urokinase kits
Sample typeSerum, Cell culture extracts, Nuclear Extracts, Purified protein
Assay typeEnzyme activity (quantitative)
Sensitivity>= 0.02 IU/ml
Species reactivityReacts with: Mammals, Other species
Urokinase Activity Assay Kit (Fluorometric) (ab174098) uses an AMC-based peptide substrate containing the recognition sequence for Urokinase. Urokinase present in the sample catalyzes the cleavage of the substrate and releases AMC, which can be easily quantified by measuring its fluorescence at Ex/Em = 350/450 nm. This sensitive assay detects very low levels of Urokinase activity in biological samples as well as purified enzymes (as low as 0.02 IU/ml).
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This product is manufactured by BioVision, an Abcam company and was previously called K728 Urokinase Activity Fluorometric Assay Kit. K728-100 is the same size as the 100 test size of ab174098.
Urokinase type plasminogen activator (uPA) is a highly restricted serine protease that converts the zymogen plasminogen to active plasmin, a broad-spectrum serine proteinase capable of degrading most of the major protein components of the extracellular matrix. Binding of uPA to its receptor and subsequent uPA mediated pericellular proteolysis are involved in many process including cell migration and tissue remodelling in angiogenesis, atherogenesis, tumor cell metastasis, and ovulation. High level of uPA is a poor prognostic marker for aggressive breast cancer, aggressive prostate cancer, bladder cancer and gastric cancer.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 100 tests Cell Lysis Buffer 1 x 25ml Human Urokinase (Standard/Positive Control) 1 vial Urokinase Assay Buffer 1 x 25ml Urokinase Substrate 1 x 0.2ml
FunctionSpecifically cleave the zymogen plasminogen to form the active enzyme plasmin.
Tissue specificityExpressed in the prostate gland and prostate cancers.
Sequence similaritiesBelongs to the peptidase S1 family.
Contains 1 EGF-like domain.
Contains 1 kringle domain.
Contains 1 peptidase S1 domain.
modificationsPhosphorylation of Ser-158 and Ser-323 abolishes proadhesive ability but does not interfere with receptor binding.
- Information by UniProt
- ATF uPA
ab174098 has been referenced in 2 publications.
- Hsieh HY et al. Hyaluronic acid on the urokinase sustained release with a hydrogel system composed of poloxamer 407: HA/P407 hydrogel system for drug delivery. PLoS One 15:e0227784 (2020). PubMed: 32160196
- Álvarez E et al. Protective, repairing and fibrinolytic effects of rivaroxaban on vascular endothelium. Br J Clin Pharmacol 84:280-291 (2018). PubMed: 28940408