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    urokinase-activity-assay-kit-fluorometric-ab174098.pdf

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Urokinase Activity Assay Kit (Fluorometric) (ab174098)

  • Datasheet
  • SDS
  • Protocol Booklet
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Urokinase Activity Standard Curves
  • Urokinase Activity Standard Curves
  • Urokinase activities in various samples

Key features and details

  • Assay type: Enzyme activity (quantitative)
  • Detection method: Fluorescent
  • Platform: Microplate reader
  • Sample type: Cell culture extracts, Nuclear Extracts, Purified protein, Serum
  • Sensitivity: 0.02 IU/ml

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Overview

  • Product name

    Urokinase Activity Assay Kit (Fluorometric)
    See all Urokinase kits
  • Detection method

    Fluorescent
  • Sample type

    Serum, Cell culture extracts, Nuclear Extracts, Purified protein
  • Assay type

    Enzyme activity (quantitative)
  • Sensitivity

    >= 0.02 IU/ml
  • Species reactivity

    Reacts with: Mammals, Other species
  • Product overview

    Urokinase Activity Assay Kit (Fluorometric) (ab174098) uses an AMC-based peptide substrate containing the recognition sequence for Urokinase. Urokinase present in the sample catalyzes the cleavage of the substrate and releases AMC, which can be easily quantified by measuring its fluorescence at Ex/Em = 350/450 nm. This sensitive assay detects very low levels of Urokinase activity in biological samples as well as purified enzymes (as low as 0.02 IU/ml).


     


    Visit our FAQs page for tips and troubleshooting.

  • Notes

    This product is manufactured by BioVision, an Abcam company and was previously called K728 Urokinase Activity Fluorometric Assay Kit. K728-100 is the same size as the 100 test size of ab174098.

    Urokinase type plasminogen activator (uPA) is a highly restricted serine protease that converts the zymogen plasminogen to active plasmin, a broad-spectrum serine proteinase capable of degrading most of the major protein components of the extracellular matrix. Binding of uPA to its receptor and subsequent uPA mediated pericellular proteolysis are involved in many process including cell migration and tissue remodelling in angiogenesis, atherogenesis, tumor cell metastasis, and ovulation. High level of uPA is a poor prognostic marker for aggressive breast cancer, aggressive prostate cancer, bladder cancer and gastric cancer.

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 100 tests
    Cell Lysis Buffer 1 x 25ml
    Human Urokinase (Standard/Positive Control) 1 vial
    Urokinase Assay Buffer 1 x 25ml
    Urokinase Substrate 1 x 0.2ml
  • Research areas

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Alzheimer's disease
    • Proteases
    • Cardiovascular
    • Blood
    • Fibrinolysis / Thrombolysis
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Kits/ Lysates/ Other
    • Kits
    • Cell Metabolism Kits
    • Coagulation Activity Assays
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Metabolism
    • Types of disease
    • Neurodegenerative disease
    • Neuroscience
    • Processes
  • Function

    Specifically cleave the zymogen plasminogen to form the active enzyme plasmin.
  • Tissue specificity

    Expressed in the prostate gland and prostate cancers.
  • Sequence similarities

    Belongs to the peptidase S1 family.
    Contains 1 EGF-like domain.
    Contains 1 kringle domain.
    Contains 1 peptidase S1 domain.
  • Post-translational
    modifications

    Phosphorylation of Ser-158 and Ser-323 abolishes proadhesive ability but does not interfere with receptor binding.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession P00749 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • ATF
    • ATF uPA
    • BDPLT5
    • Plasminogen activator
    • Plasminogen activator urinary
    • Plasminogen activator urokinase
    • PLAU
    • QPD
    • u PA
    • U plasminogen activator
    • u-PA
    • U-plasminogen activator
    • uPA
    • URK
    • UROK_HUMAN
    • Urokinase plasminogen activator
    • Urokinase type plasminogen activator
    • Urokinase type plasminogen activator precursor
    • Urokinase-type plasminogen activator chain B
    see all

Images

  • Urokinase Activity Standard Curves
    Urokinase Activity Standard Curves

    This is example data only.

  • Urokinase Activity Standard Curves
    Urokinase Activity Standard Curves

    This is example data only.

  • Urokinase activities in various samples
    Urokinase activities in various samples

    Urokinase activities of Jurkat cell extract [10 µl], Hela nuclear extract [10 µl] and Pooled human serum (10 µl). Assays were performed following the kit protocol. This is example data only.

Protocols

  • Protocol Booklet

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (2)

Publishing research using ab174098? Please let us know so that we can cite the reference in this datasheet.

ab174098 has been referenced in 2 publications.

  • Hsieh HY  et al. Hyaluronic acid on the urokinase sustained release with a hydrogel system composed of poloxamer 407: HA/P407 hydrogel system for drug delivery. PLoS One 15:e0227784 (2020). PubMed: 32160196
  • Álvarez E  et al. Protective, repairing and fibrinolytic effects of rivaroxaban on vascular endothelium. Br J Clin Pharmacol 84:280-291 (2018). PubMed: 28940408

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