Urokinase Activity Assay Kit (Fluorometric) (ab174098)

Overview

  • Product name
    Urokinase Activity Assay Kit (Fluorometric)
    See all Urokinase type Plasminogen Activator kits
  • Detection method
    Fluorescent
  • Sample type
    Serum, Cell culture extracts, Nuclear Extracts, Purified protein
  • Assay type
    Quantitative
  • Sensitivity
    >= 0.02 IU/ml
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Other species, Mammals
  • Product overview

    Urokinase Activity Assay Kit (Fluorometric) (ab174098) uses an AMC-based peptide substrate containing the recognition sequence for Urokinase. Urokinase present in the sample catalyzes the cleavage of the substrate and releases AMC, which can be easily quantified by measuring its fluorescence at Ex/Em = 350/450 nm. This sensitive assay detects very low levels of Urokinase activity in biological samples as well as purified enzymes (as low as 0.02 IU/ml).

     

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  • Notes

    Urokinase type plasminogen activator (uPA) is a highly restricted serine protease that converts the zymogen plasminogen to active plasmin, a broad-spectrum serine proteinase capable of degrading most of the major protein components of the extracellular matrix. Binding of uPA to its receptor and subsequent uPA mediated pericellular proteolysis are involved in many process including cell migration and tissue remodelling in angiogenesis, atherogenesis, tumor cell metastasis, and ovulation. High level of uPA is a poor prognostic marker for aggressive breast cancer, aggressive prostate cancer, bladder cancer and gastric cancer.

  • Platform
    Microplate

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 100 tests
    Cell Lysis Buffer 1 x 25ml
    Human Urokinase (Standard/Positive Control) 1 vial
    Urokinase Assay Buffer 1 x 25ml
    Urokinase Substrate 1 x 0.2ml
  • Research areas
  • Function
    Specifically cleaves the zymogen plasminogen to form the active enzyme plasmin.
  • Tissue specificity
    Expressed in the prostate gland and prostate cancers.
  • Involvement in disease
    Quebec platelet disorder
  • Sequence similarities
    Belongs to the peptidase S1 family.
    Contains 1 EGF-like domain.
    Contains 1 kringle domain.
    Contains 1 peptidase S1 domain.
  • Post-translational
    modifications
    Phosphorylation of Ser-158 and Ser-323 abolishes proadhesive ability but does not interfere with receptor binding.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Alternative names
    • ATF
    • BDPLT5
    • EC 3.4.21
    • EC 3.4.21.73
    • Plasminogen Activator, Urinary
    • Plasminogen Activator, Urokinase
    • PLAU
    • QPD
    • U-PA
    • U-plasminogen activator
    • uPA
    • URK
    • UROK_HUMAN
    • Urokinase-Type Plasminogen Activator
    • Urokinase-type plasminogen activator chain B
    see all

Images

  • This is example data only.

  • This is example data only.

  • Urokinase activities of Jurkat cell extract [10 µl], Hela nuclear extract [10 µl] and Pooled human serum (10 µl). Assays were performed following the kit protocol. This is example data only.

Protocols

References

ab174098 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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