Key features and details
- Goat polyclonal to Urokinase
- Suitable for: Dot blot, IHC-Fr, IHC-P, ELISA, WB, RIA
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Urokinase antibody
See all Urokinase primary antibodies
DescriptionGoat polyclonal to Urokinase
SpecificityThe antibody is highly specific for urokinase, no crossreactions were found with other serine proteases (tissue plasminogen activator, plasmin, trypsin, chymotrypsin, elastase, thrombin).
Tested applicationsSuitable for: Dot blot, IHC-Fr, IHC-P, ELISA, WB, RIAmore details
Species reactivityReacts with: Human
Extensively purified HMW-tcuPA and LMW-scuPA (mixture 50/50), isolated from urine (Human).
ab24122 binds to human HMW-scuPA (54 kDa), HMW-tsuPA (52 kDa) and LMW-scuPA (33 kDa) with high apparent affinity (Kd= 0.1 nM) completely inhibiting the ability of urokinase to activate plasminogen. Urokinase reaction with synthetic substrate S2444 is also inhibited. Human whole plasma or plasma proteins do not interfere with its binding to urokinase.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferConstituent: PBS
Concentration information loading...
PurityImmunogen affinity purified
Primary antibody notesab24122 binds to human HMW-scuPA (54 kDa), HMW-tsuPA (52 kDa) and LMW-scuPA (33 kDa) with high apparent affinity (Kd= 0.1 nM) completely inhibiting the ability of urokinase to activate plasminogen. Urokinase reaction with synthetic substrate S2444 is also inhibited. Human whole plasma or plasma proteins do not interfere with its binding to urokinase.
Our Abpromise guarantee covers the use of ab24122 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Dot blot||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 54, 52 , 33 kDa.|
|RIA||Use at an assay dependent concentration.|
FunctionSpecifically cleave the zymogen plasminogen to form the active enzyme plasmin.
Tissue specificityExpressed in the prostate gland and prostate cancers.
Sequence similaritiesBelongs to the peptidase S1 family.
Contains 1 EGF-like domain.
Contains 1 kringle domain.
Contains 1 peptidase S1 domain.
modificationsPhosphorylation of Ser-158 and Ser-323 abolishes proadhesive ability but does not interfere with receptor binding.
- Information by UniProt
- ATF antibody
- ATF uPA antibody
- BDPLT5 antibody
ab24122 has been referenced in 1 publication.
- Li C et al. MiR-10a and miR-181c regulate collagen type I generation in hypertrophic scars by targeting PAI-1 and uPA. FEBS Lett 589:380-9 (2015). PubMed: 25554417