Overview

  • Product name

    Anti-Urokinase antibody [EPR6273]
    See all Urokinase primary antibodies
  • Description

    Rabbit monoclonal [EPR6273] to Urokinase
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Urokinase aa 50-150. The exact sequence is proprietary.
    Database link: P00749

  • Positive control

    • WB: PC-3, LnCaP and MCF7 cell lysates. IHC-P: Human kidney tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab133563 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 49 kDa.

For unpurified use at 1/100.

IHC-P 1/150. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

  • Application notes
    Is unsuitable for Flow Cyt or ICC/IF.
  • Target

    • Function

      Specifically cleave the zymogen plasminogen to form the active enzyme plasmin.
    • Tissue specificity

      Expressed in the prostate gland and prostate cancers.
    • Sequence similarities

      Belongs to the peptidase S1 family.
      Contains 1 EGF-like domain.
      Contains 1 kringle domain.
      Contains 1 peptidase S1 domain.
    • Post-translational
      modifications

      Phosphorylation of Ser-158 and Ser-323 abolishes proadhesive ability but does not interfere with receptor binding.
    • Cellular localization

      Secreted.
    • Information by UniProt
    • Database links

    • Alternative names

      • ATF antibody
      • ATF uPA antibody
      • BDPLT5 antibody
      • Plasminogen activator antibody
      • Plasminogen activator urinary antibody
      • Plasminogen activator urokinase antibody
      • PLAU antibody
      • QPD antibody
      • u PA antibody
      • U plasminogen activator antibody
      • u-PA antibody
      • U-plasminogen activator antibody
      • uPA antibody
      • URK antibody
      • UROK_HUMAN antibody
      • Urokinase plasminogen activator antibody
      • Urokinase type plasminogen activator antibody
      • Urokinase type plasminogen activator precursor antibody
      • Urokinase-type plasminogen activator chain B antibody
      see all

    Images

    • All lanes : Anti-Urokinase antibody [EPR6273] (ab133563) at 1/100 dilution (unpurified)

      Lane 1 : LnCap cell lysate
      Lane 2 : PC-3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size: 49 kDa
      Observed band size: 49 kDa



      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling Urokinase with purified ab133563 at 1/150. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with Hematoxylin.

    • All lanes : Anti-Urokinase antibody [EPR6273] (ab133563) at 1/1300 dilution (purified)

      Lane 1 : LnCaP cell lysate
      Lane 2 : PC-3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size: 49 kDa
      Observed band size: 49 kDa



      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • All lanes : Anti-Urokinase antibody [EPR6273] (ab133563) at 1/1000 dilution (unpurified)

      Lane 1 : PC-3 cell lysate
      Lane 2 : LnCaP cell lysate
      Lane 3 : MCF7 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

      Developed using the ECL technique.

      Predicted band size: 49 kDa

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Burchall GF  et al. Expression of the plasminogen system in the physiological mouse ovary and in the pathological polycystic ovary syndrome (PCOS) state. Reprod Biol Endocrinol 17:33 (2019). Read more (PubMed: 30878040) »
    • Yang K  et al. Identification of SERPINE1, PLAU and ACTA1 as biomarkers of head and neck squamous cell carcinoma based on integrated bioinformatics analysis. Int J Clin Oncol N/A:N/A (2019). Read more (PubMed: 30937621) »
    See all 3 Publications for this product

    Customer reviews and Q&As

    Answer

    According to the literature, the ˜54/49kD can be detected under reducing conditions and it could represent the single chain pro-uPA. Also, looking at some of the literatures’ data, the detection of the pro-uPA, 18 and 33kD bands will vary from one sample to another.

    Furthermore, it’s possible that the reducing agent could make a difference too. For example, to test this antibody, we used BME, which is a weaker reducing agent than DTT and so this may account for the single band at 49 kDa being observed.

    Read More

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