Urokinase type Plasminogen Activator Human Chromogenic Activity Assay Kit - Direct Assay (ab108916)

Overview

  • Product name

    Urokinase type Plasminogen Activator Human Chromogenic Activity Assay Kit - Direct Assay
    See all Urokinase type Plasminogen Activator kits
  • Detection method

    Colorimetric
  • Sample type

    Cell culture supernatant, Plasma
  • Assay type

    Enzyme activity (quantitative)
  • Sensitivity

    < 0.1 IU/ml
  • Species reactivity

    Reacts with: Human
  • Product overview

    Urokinase type plasminogen activator (uPA) is a highly restricted serine protease that converts the zymogen plasminogen to active plasmin, a broad-spectrum serine proteinase capable of degrading most of the major protein components of the extracellular matrix. Binding of uPA to its receptor and subsequent uPA mediated pericellular proteolysis are involved in many process including cell migration and tissue remodeling in angiogeenesis, atherogenesis, tumor cell metastasis, and ovulation. High level of uPA is a poor prognostic marker for aggressive breast cancer, aggressive prostate cancer, bladder cancer and gastric cancer.


    ab108916 uPA Human Chromogenic Activity Assay Kit is developed to determine Human uPA activity in plasma and cell culture supernatants. The amidolytic activity of uPA is quantitated using a highly specific uPA substrate releasing a yellow para-nitroaniline (pNA) chromophore. The change in absorbance of the pNA at 405 nm is directly proportional to the uPA enzymatic activity.


    The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

  • Platform

    Microplate reader

Properties

Images

  • uPA Chromogenic Activity Standard Curve (For Low uPA Activity Samples)
  • uPA Chromogenic Activity Standard Curve (For High uPA Activity Samples)

Protocols

References

ab108916 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer



For uPA activity kits, we have two types of kit. One is ab108916 which is direct using uPA substrate to generate the color. The uPA substrate is specifically designed for its specificity for uPA. This assay can differentiate uPA activity vs. tPA activity.

On the other hand, we also have an indirect uPA activity kit which is ab108915. This kit is using a plasmin substrate to measure uPA activity.

ab108916 can differentiate uPA vs. tPA activity, however, it is not as sensitive as ab108915 which is an indirect assay. ab108915 is more sensitive assay but is no able to differentiate uPA vs. tPA activity. We provide uPA standard for indirect assay is for reference of uPA activity.

If a cell culture sample specific for uPA expression is used, ab108915 will works well. However, if a plasma or serum sample is used, it is difficult to differentiate from tPA activity. But as the uPA activity accounts for at least 50 % of activity for cleave plasminogen to plasmin, the kit can at least provide a reference for uPA activity when samples are compared although we would recommend ab108916 if the samples have enough uPA.

Click here (or use the following: https://www.abcam.com/index.html?datasheet=108915).

Click here (or use the following: https://www.abcam.com/index.html?datasheet=108916).

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