Anti-uronyl 2 sulfotransferase/UST antibody (ab137624)
Key features and details
- Rabbit polyclonal to uronyl 2 sulfotransferase/UST
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-uronyl 2 sulfotransferase/UST antibody -
Description
Rabbit polyclonal to uronyl 2 sulfotransferase/UST -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow
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Immunogen
Recombinant fragment corresponding to Human uronyl 2 sulfotransferase/UST aa 56-344.
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Positive control
- 293T whole cell lysate; SW480 xenograft tissue. H1299 and HeLaS3 cell lines.
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General notes
This product was previously labelled as uronyl 2 sulfotransferase
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137624 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB |
1/500 - 1/3000. Predicted molecular weight: 48 kDa.
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| Notes |
|---|
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IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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WB
1/500 - 1/3000. Predicted molecular weight: 48 kDa. |
Target
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Function
Sulfotransferase that catalyzes the transfer of sulfate to the position 2 of uronyl residues. Has mainly activity toward iduronyl residues in dermatan sulfate, and weaker activity toward glucuronyl residues of chondroitin sulfate. Has no activity toward desulfated N-resulfated heparin. -
Tissue specificity
Widely expressed. -
Sequence similarities
Belongs to the sulfotransferase 3 family. -
Cellular localization
Golgi apparatus membrane. - Information by UniProt
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Database links
- Entrez Gene: 10090 Human
- Entrez Gene: 338362 Mouse
- Entrez Gene: 361450 Rat
- Omim: 610752 Human
- SwissProt: Q9Y2C2 Human
- SwissProt: Q8BUB6 Mouse
- Unigene: 657370 Human
- Unigene: 41163 Mouse
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Alternative names
- 2OST antibody
- DS2ST antibody
- Uronyl 2-sulfotransferase antibody
see all
Images
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Western blot - Anti-uronyl 2 sulfotransferase/UST antibody (ab137624)Anti-uronyl 2 sulfotransferase/UST antibody (ab137624) at 1/1000 dilution + 293T whole cell lysate at 30 µg
Predicted band size: 48 kDa
Gel: 10% -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-uronyl 2 sulfotransferase/UST antibody (ab137624)Immunohistochemical analysis, using ab137624 at 1:500 dilution, on paraffin-embedded SW480 xenograft.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab137624 has been referenced in 2 publications.
- Wang L et al. Serious Selenium Deficiency in the Serum of Patients with Kashin-Beck Disease and the Effect of Nano-Selenium on Their Chondrocytes. Biol Trace Elem Res 194:96-104 (2020). PubMed: 31175635
- Han J et al. Altered expression of chondroitin sulfate structure modifying sulfotransferases in the articular cartilage from adult osteoarthritis and Kashin-Beck disease. Osteoarthritis Cartilage 25:1372-1375 (2017). PubMed: 28274888