Recombinant Anti-Uroplakin Ib/UPIb antibody [2N23] (ab237777)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [2N23] to Uroplakin Ib/UPIb
- Suitable for: IHC-P, IHC-Fr
- Reacts with: Mouse, Rat
Related conjugates and formulations
Overview
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Product name
Anti-Uroplakin Ib/UPIb antibody [2N23]
See all Uroplakin Ib/UPIb primary antibodies -
Description
Mouse monoclonal [2N23] to Uroplakin Ib/UPIb -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, IHC-Frmore details
Unsuitable for: WB -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Mouse and rat bladder tissue. Rat kidney tissue. IHC-Fr: Mouse and rat kidney tissue.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
2N23 -
Isotype
IgG2b -
Research areas
Associated products
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Alkaline Phosphatase detection kit
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Alternative Versions
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab237777 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
Use a concentration of 0.03 - 0.1 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
|
IHC-Fr |
Use a concentration of 3.94 µg/ml.
Perform heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). |
Notes |
---|
IHC-P
Use a concentration of 0.03 - 0.1 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IHC-Fr
Use a concentration of 3.94 µg/ml. Perform heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). |
Target
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Function
Component of the asymmetric unit membrane (AUM); a highly specialized biomembrane elaborated by terminally differentiated urothelial cells. May play an important role in normal bladder epithelial physiology, possibly in regulating membrane permeability of superficial umbrella cells or in stabilizing the apical membrane through AUM/cytoskeletal interactions. -
Tissue specificity
Bladder epithelium. -
Sequence similarities
Belongs to the tetraspanin (TM4SF) family. -
Post-translational
modificationsN-glycosylated with high-mannose oligosaccharides. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 22268 Mouse
- Entrez Gene: 303924 Rat
- SwissProt: Q9Z2C6 Mouse
- SwissProt: Q566D0 Rat
- Unigene: 130793 Mouse
- Unigene: 9134 Rat
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Alternative names
- Tetraspanin-20 antibody
- Tspan-20 antibody
- TSPAN20 antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling Uroplakin Ib/UPIb with ab237777 at 0.099µg/ml, followed by ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Positive staining on rat kidney tissue is observed. The section was incubated with ab237777 for 30 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. -
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat kidney tissue labeling Uroplakin Ib/UPIb with ab237777 at 1/50 dilution, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary at 3.94µg/ml (Green). Positive staining on rat kidney is observed. Nuclear counterstain is DAPI (Blue).
Secondary antibody only control: Used PBS instead of primary antibody, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary at a 1/1000 dilution.Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
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Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse kidney tissue labeling Uroplakin Ib/UPIb with ab237777 at 3.94µg/ml, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary at a 1/1000 dilution (Green). Positive staining on mouse kidney is observed. Nuclear counterstain is DAPI (Blue).
Secondary antibody only control: Used PBS instead of primary antibody, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary at a 1/1000 dilution.Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
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Immunohistochemical analysis of paraffin-embedded rat bladder tissue labeling Uroplakin Ib/UPIb with ab237777 at 0.099µg/ml, followed by ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Positive staining on rat bladder tissue is observed. The section was incubated with ab237777 for 30 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. -
Immunohistochemical analysis of paraffin-embedded mouse bladder tissue labeling Uroplakin Ib/UPIb with ab237777 at 0.099µg/ml, followed by ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Positive staining on mouse bladder tissue is observed. The section was incubated with ab237777 for 30 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. -
Immunohistochemical analysis of paraffin-embedded mouse bladder tissue labeling Uroplakin Ib/UPIb with ab237777 at 0.032µg/ml, followed by Anti-Mouse IgG2b antibody [RM108] (ab190482). Positive staining on mouse bladder tissue is observed. The section was incubated with ab237777 for 30 mins at room temperature, followed by anti-mouse IgG2b IgG antibody (ab190482) for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument.
Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, followed by Anti-Mouse IgG2b antibody [RM108] (ab190482).
Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes.
-
Immunohistochemical analysis of paraffin-embedded rat bladder tissue labeling Uroplakin Ib/UPIb with ab237777 at 0.032µg/ml, followed by Anti-Mouse IgG2b antibody [RM108] (ab190482). Positive staining on rat bladder tissue is observed. The section was incubated with ab237777 for 30 mins at room temperature, followed by anti-mouse IgG2b IgG antibody (ab190482) for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument.
Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, followed by Anti-Mouse IgG2b antibody [RM108] (ab190482).
Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab237777 has been referenced in 1 publication.
- Li N & Liu Y LARP7 alleviates psoriasis symptoms in mice by regulating the SIRT1/NF-κB signaling pathway. Allergol Immunopathol (Madr) 51:140-145 (2023). PubMed: 36617833