Recombinant

Recombinant Anti-Uroplakin Ib/UPIb antibody [2N23] (ab237777)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uroplakin Ib/UPIb antibody [2N23] (ab237777)
  • Immunohistochemistry (Frozen sections) - Anti-Uroplakin Ib/UPIb antibody [2N23] (ab237777)
  • Immunohistochemistry (Frozen sections) - Anti-Uroplakin Ib/UPIb antibody [2N23] (ab237777)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uroplakin Ib/UPIb antibody [2N23] (ab237777)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uroplakin Ib/UPIb antibody [2N23] (ab237777)
  • Anti-Uroplakin Ib/UPIb antibody [2N23] (ab237777)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [2N23] to Uroplakin Ib/UPIb
  • Suitable for: IHC-P, IHC-Fr
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-Uroplakin Ib/UPIb antibody [2N23]
    See all Uroplakin Ib/UPIb primary antibodies

  • Description

    Mouse monoclonal [2N23] to Uroplakin Ib/UPIb

  • Host species

    Mouse

  • Tested applications

    Suitable for: IHC-P, IHC-Frmore details
    Unsuitable for: WB

  • Species reactivity

    Reacts with: Mouse, Rat

  • Immunogen

    Synthetic peptide. This information is considered to be commercially sensitive.

  • Positive control

    • IHC-P: Mouse and rat bladder tissue. Rat kidney tissue. IHC-Fr: Mouse and rat kidney tissue.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a different buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    2N23

  • Isotype

    IgG2b

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab237777 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 0.099 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC-Fr Use a concentration of 3.94 µg/ml.

Perform heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
  • Application notes
    Is unsuitable for WB.

    • Target

    • Function

      Component of the asymmetric unit membrane (AUM); a highly specialized biomembrane elaborated by terminally differentiated urothelial cells. May play an important role in normal bladder epithelial physiology, possibly in regulating membrane permeability of superficial umbrella cells or in stabilizing the apical membrane through AUM/cytoskeletal interactions.

    • Tissue specificity

      Bladder epithelium.

    • Sequence similarities

      Belongs to the tetraspanin (TM4SF) family.

    • Post-translational
      modifications

      N-glycosylated with high-mannose oligosaccharides.

    • Cellular localization

      Membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • Tetraspanin-20 antibody
        • Tspan-20 antibody
        • TSPAN20 antibody
        • UP1b antibody
        • UPIb antibody
        • UPK1B antibody
        • UPK1B_HUMAN antibody
        • Uroplakin Ib antibody
        • Uroplakin-1b antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uroplakin Ib/UPIb antibody [2N23] (ab237777)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uroplakin Ib/UPIb antibody [2N23] (ab237777)

        Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling Uroplakin Ib/UPIb with ab237777 at 0.099µg/ml, followed by ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Positive staining on rat kidney tissue is observed. The section was incubated with ab237777 for 30 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
        Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).
        Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

      • Immunohistochemistry (Frozen sections) - Anti-Uroplakin Ib/UPIb antibody [2N23] (ab237777)
        Immunohistochemistry (Frozen sections) - Anti-Uroplakin Ib/UPIb antibody [2N23] (ab237777)

        Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat kidney tissue labeling Uroplakin Ib/UPIb with ab237777 at 1/50 dilution, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary at 3.94µg/ml (Green). Positive staining on rat kidney is observed. Nuclear counterstain is DAPI (Blue).
        Secondary antibody only control: Used PBS instead of primary antibody, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary at a 1/1000 dilution.

        Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

      • Immunohistochemistry (Frozen sections) - Anti-Uroplakin Ib/UPIb antibody [2N23] (ab237777)
        Immunohistochemistry (Frozen sections) - Anti-Uroplakin Ib/UPIb antibody [2N23] (ab237777)

        Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse kidney tissue labeling Uroplakin Ib/UPIb with ab237777 at 3.94µg/ml, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary at a 1/1000 dilution (Green). Positive staining on mouse kidney is observed. Nuclear counterstain is DAPI (Blue).
        Secondary antibody only control: Used PBS instead of primary antibody, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary at a 1/1000 dilution.

        Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uroplakin Ib/UPIb antibody [2N23] (ab237777)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uroplakin Ib/UPIb antibody [2N23] (ab237777)

        Immunohistochemical analysis of paraffin-embedded rat bladder tissue labeling Uroplakin Ib/UPIb with ab237777 at 0.099µg/ml, followed by ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Positive staining on rat bladder tissue is observed. The section was incubated with ab237777 for 30 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
        Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).
        Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uroplakin Ib/UPIb antibody [2N23] (ab237777)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uroplakin Ib/UPIb antibody [2N23] (ab237777)

        Immunohistochemical analysis of paraffin-embedded mouse bladder tissue labeling Uroplakin Ib/UPIb with ab237777 at 0.099µg/ml, followed by ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Positive staining on mouse bladder tissue is observed. The section was incubated with ab237777 for 30 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
        Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).
        Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

      • Anti-Uroplakin Ib/UPIb antibody [2N23] (ab237777)
        Anti-Uroplakin Ib/UPIb antibody [2N23] (ab237777)

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      References (0)

      Publishing research using ab237777? Please let us know so that we can cite the reference in this datasheet.

      ab237777 has not yet been referenced specifically in any publications.

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