Overview

  • Product name

    Anti-Uroplakin Ib/UPIb antibody
    See all Uroplakin Ib/UPIb primary antibodies
  • Description

    Rabbit polyclonal to Uroplakin Ib/UPIb
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human Uroplakin Ib/UPIb aa 166-196 (internal sequence) conjugated to keyhole limpet haemocyanin.

  • Positive control

    • K562 cell lysate, Human Brain Tissue
  • General notes

     This product was previously labelled as Uroplakin Ib

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    This antibody is purified through a protein A column, followed by peptide affinity purification.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab102961 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

WB 1/1000. Predicted molecular weight: 30 kDa.
IHC-P 1/50 - 1/100.

Target

  • Function

    Component of the asymmetric unit membrane (AUM); a highly specialized biomembrane elaborated by terminally differentiated urothelial cells. May play an important role in normal bladder epithelial physiology, possibly in regulating membrane permeability of superficial umbrella cells or in stabilizing the apical membrane through AUM/cytoskeletal interactions.
  • Tissue specificity

    Bladder epithelium.
  • Sequence similarities

    Belongs to the tetraspanin (TM4SF) family.
  • Post-translational
    modifications

    N-glycosylated with high-mannose oligosaccharides.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Tetraspanin-20 antibody
    • Tspan-20 antibody
    • TSPAN20 antibody
    • UP1b antibody
    • UPIb antibody
    • UPK1B antibody
    • UPK1B_HUMAN antibody
    • Uroplakin Ib antibody
    • Uroplakin-1b antibody
    see all

Images

  • Immunohistochemical staining of formalin fixed and paraffin embedded brain tissue followed by HRP-conjugated secondary antibody and DAB staining.

  • Anti-Uroplakin Ib/UPIb antibody (ab102961) at 1/100 dilution + K562 cell lysate at 35 µg

    Predicted band size: 30 kDa

  • Flow cytometric analysis of K562 cells (green histogram) using ab102961 compared to a negative control cell (blue histogram). FITC-conjugated goat anti-rabbit secondary antibodies were used for the analysis.

References

ab102961 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step
None
Sample
Human Tissue sections (gingiva)
Specification
gingiva
Permeabilization
No
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Dec 16 2013

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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