Recombinant Anti-Uroplakin Ib/UPIb antibody [EPR14451] (ab183503)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14451] to Uroplakin Ib/UPIb
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Uroplakin Ib/UPIb antibody [EPR14451]
See all Uroplakin Ib/UPIb primary antibodies -
Description
Rabbit monoclonal [EPR14451] to Uroplakin Ib/UPIb -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human fetal brain tissue lysate, K562 cell line lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Allow to warm to room temp and agitate gently before aliquotting. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14451 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab183503 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/10000 - 1/50000. Detects a band of approximately 25 kDa (predicted molecular weight: 30 kDa).
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Notes |
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WB
1/10000 - 1/50000. Detects a band of approximately 25 kDa (predicted molecular weight: 30 kDa). |
Target
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Function
Component of the asymmetric unit membrane (AUM); a highly specialized biomembrane elaborated by terminally differentiated urothelial cells. May play an important role in normal bladder epithelial physiology, possibly in regulating membrane permeability of superficial umbrella cells or in stabilizing the apical membrane through AUM/cytoskeletal interactions. -
Tissue specificity
Bladder epithelium. -
Sequence similarities
Belongs to the tetraspanin (TM4SF) family. -
Post-translational
modificationsN-glycosylated with high-mannose oligosaccharides. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 7348 Human
- Entrez Gene: 22268 Mouse
- Entrez Gene: 303924 Rat
- Omim: 602380 Human
- SwissProt: O75841 Human
- SwissProt: Q9Z2C6 Mouse
- SwissProt: Q566D0 Rat
- Unigene: 271580 Human
see all -
Alternative names
- Tetraspanin-20 antibody
- Tspan-20 antibody
- TSPAN20 antibody
see all
Images
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Anti-Uroplakin Ib/UPIb antibody [EPR14451] (ab183503) at 1/100000 dilution + K562 cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution
Predicted band size: 30 kDa
Observed band size: 25 kDa why is the actual band size different from the predicted? -
Anti-Uroplakin Ib/UPIb antibody [EPR14451] (ab183503) at 1/20000 dilution + Human fetal brain tissue lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution
Predicted band size: 30 kDa
Observed band size: 25 kDa why is the actual band size different from the predicted?
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab183503 has been referenced in 1 publication.
- Hu M et al. Effect of α-tocopherol in alleviating the lipopolysaccharide-induced acute lung injury via inhibiting nuclear factor kappa-B signaling pathways. Bioengineered 13:3958-3968 (2022). PubMed: 35112986