Anti-Uroplakin Ib/UPIb antibody [UPK1B/3102] - BSA and Azide free (ab263459)
Key features and details
- Mouse monoclonal [UPK1B/3102] to Uroplakin Ib/UPIb - BSA and Azide free
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Uroplakin Ib/UPIb antibody [UPK1B/3102] - BSA and Azide free
See all Uroplakin Ib/UPIb primary antibodies -
Description
Mouse monoclonal [UPK1B/3102] to Uroplakin Ib/UPIb - BSA and Azide free -
Host species
Mouse -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human Uroplakin Ib/UPIb aa 106-229. The exact sequence is proprietary.
Database link: O75841 -
Positive control
- IHC-P: Human urothelial carcinoma tissue.
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General notes
ab263459 is the carrier-free version of ab263455.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A/G purified -
Purification notes
Purified from bioreactor concentrate. -
Clonality
Monoclonal -
Clone number
UPK1B/3102 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab263459 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Incubate with primary antibody for 30 mins at RT. |
Notes |
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IHC-P
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Incubate with primary antibody for 30 mins at RT. |
Target
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Function
Component of the asymmetric unit membrane (AUM); a highly specialized biomembrane elaborated by terminally differentiated urothelial cells. May play an important role in normal bladder epithelial physiology, possibly in regulating membrane permeability of superficial umbrella cells or in stabilizing the apical membrane through AUM/cytoskeletal interactions. -
Tissue specificity
Bladder epithelium. -
Sequence similarities
Belongs to the tetraspanin (TM4SF) family. -
Post-translational
modificationsN-glycosylated with high-mannose oligosaccharides. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 7348 Human
- Omim: 602380 Human
- SwissProt: O75841 Human
- Unigene: 271580 Human
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Alternative names
- Tetraspanin-20 antibody
- Tspan-20 antibody
- TSPAN20 antibody
see all
Images
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Formalin-fixed, paraffin-embedded human urothelial carcinoma tissue stained for Uroplakin Ib/UPIb using ab263455 at 2 µg/ml in immunohistochemistry.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
This image was produced using the same clone but different formulation, PBS, sodium azide and BSA ab263455.
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Formalin-fixed, paraffin-embedded human urothelial carcinoma tissue stained for Uroplakin Ib/UPIb using ab263455 at 2 µg/ml in immunohistochemistry.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
This image was produced using the same clone but different formulation, PBS, sodium azide and BSA ab263455.
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Formalin-fixed, paraffin-embedded human urothelial carcinoma tissue stained for Uroplakin Ib/UPIb using ab263455 at 2 µg/ml in immunohistochemistry.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
This image was produced using the same clone but different formulation, PBS, sodium azide and BSA ab263455.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab263459 has not yet been referenced specifically in any publications.