Anti-Uroplakin III antibody (ab82173)
- Datasheet
- References (2)
- Protocols
Overview
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Product name
Anti-Uroplakin III antibody
See all Uroplakin III primary antibodies -
Description
Rabbit polyclonal to Uroplakin III -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Uroplakin III aa 250 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: O75631 -
Positive control
- Human Bladder
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab82173 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| WB | Use at an assay dependent concentration. |
Target
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Function
Component of the asymmetric unit membrane (AUM); a highly specialized biomembrane elaborated by terminally differentiated urothelial cells. May play an important role in AUM-cytoskeleton interaction in terminally differentiated urothelial cells. It also contributes to the formation of urothelial glycocalyx which may play an important role in preventing bacterial adherence. -
Tissue specificity
Expressed in ureter. -
Involvement in disease
Defects in UPK3A are a cause of renal adysplasia (RADYS) [MIM:191830]; also known as renal agenesis or renal aplasia. Renal agenesis refers to the absence of one (unilateral) or both (bilateral) kidneys at birth. Bilateral renal agenesis belongs to a group of perinatally lethal renal diseases, including severe bilateral renal dysplasia, unilateral renal agenesis with contralateral dysplasia and severe obstructive uropathy. -
Sequence similarities
Belongs to the uroplakin-3 family. -
Cellular localization
Endoplasmic reticulum membrane. Heterodimer formation with UPK1B is a prerequisite to exit out of the endoplasmic reticulum (ER). - Information by UniProt
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Database links
- Entrez Gene: 7380 Human
- Omim: 611559 Human
- SwissProt: O75631 Human
- Unigene: 632787 Human
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Alternative names
- MGC119178 antibody
- OTTHUMP00000028951 antibody
- UP III antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uroplakin III antibody (ab82173)Formalin-fixed, paraffin-embedded section of Human bladder stained with ab82173 at 1/100 dilution. Incubate primary antibody (ab82173) for 10 minutes at RT. -
Western blot - Anti-Uroplakin III antibody (ab82173)Anti-Uroplakin III antibody (ab82173) at 1/100 dilution + lysate prepared from bladder
References
This product has been referenced in:
- Hou T et al. Aberrant differentiation of urothelial cells in patients with ureteropelvic junction obstruction. Int J Clin Exp Pathol 7:5837-45 (2014). IF ; Human . Read more (PubMed: 25337225) »
- Hicks AN et al. Lack of nicotinamide mononucleotide adenylyltransferase 2 (Nmnat2): Consequences for mouse bladder development and function. Neurourol Urodyn : (2013). Read more (PubMed: 23371862) »
