Anti-Uroplakin III antibody (ab82173)
Key features and details
- Rabbit polyclonal to Uroplakin III
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Uroplakin III antibody
See all Uroplakin III primary antibodies -
Description
Rabbit polyclonal to Uroplakin III -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Uroplakin III aa 250 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: O75631 -
Positive control
- Human Bladder
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General notes
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab82173 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| WB | Use at an assay dependent concentration. |
Target
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Function
Component of the asymmetric unit membrane (AUM); a highly specialized biomembrane elaborated by terminally differentiated urothelial cells. May play an important role in AUM-cytoskeleton interaction in terminally differentiated urothelial cells. It also contributes to the formation of urothelial glycocalyx which may play an important role in preventing bacterial adherence. -
Tissue specificity
Expressed in ureter. -
Involvement in disease
Defects in UPK3A are a cause of renal adysplasia (RADYS) [MIM:191830]; also known as renal agenesis or renal aplasia. Renal agenesis refers to the absence of one (unilateral) or both (bilateral) kidneys at birth. Bilateral renal agenesis belongs to a group of perinatally lethal renal diseases, including severe bilateral renal dysplasia, unilateral renal agenesis with contralateral dysplasia and severe obstructive uropathy. -
Sequence similarities
Belongs to the uroplakin-3 family. -
Cellular localization
Endoplasmic reticulum membrane. Heterodimer formation with UPK1B is a prerequisite to exit out of the endoplasmic reticulum (ER). - Information by UniProt
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Database links
- Entrez Gene: 7380 Human
- Omim: 611559 Human
- SwissProt: O75631 Human
- Unigene: 632787 Human
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Alternative names
- MGC119178 antibody
- OTTHUMP00000028951 antibody
- UP III antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uroplakin III antibody (ab82173)Formalin-fixed, paraffin-embedded section of Human bladder stained with ab82173 at 1/100 dilution. Incubate primary antibody (ab82173) for 10 minutes at RT.
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Western blot - Anti-Uroplakin III antibody (ab82173)Anti-Uroplakin III antibody (ab82173) at 1/100 dilution + lysate prepared from bladder
Protocols
References (3)
ab82173 has been referenced in 3 publications.
- Jafree DJ et al. Spatiotemporal dynamics and heterogeneity of renal lymphatics in mammalian development and cystic kidney disease. Elife 8:N/A (2019). PubMed: 31808745
- Hou T et al. Aberrant differentiation of urothelial cells in patients with ureteropelvic junction obstruction. Int J Clin Exp Pathol 7:5837-45 (2014). IF ; Human . PubMed: 25337225
- Hicks AN et al. Lack of nicotinamide mononucleotide adenylyltransferase 2 (Nmnat2): Consequences for mouse bladder development and function. Neurourol Urodyn : (2013). PubMed: 23371862
